A5029300973- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Microbial Natural Products and Biosynthesis
- Systemic Lupus Erythematosus Research
- Bioactive Compounds and Antitumor Agents
- Estrogen and related hormone effects
- SARS-CoV-2 and COVID-19 Research
- Cardiovascular Disease and Adiposity
- Salivary Gland Disorders and Functions
- Marine Sponges and Natural Products
- Urticaria and Related Conditions
- Adipose Tissue and Metabolism
- Asthma and respiratory diseases
- Protein Tyrosine Phosphatases
- Adipokines, Inflammation, and Metabolic Diseases
- DNA Repair Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Viral Infections and Immunology Research
- PARP inhibition in cancer therapy
- Peripheral Neuropathies and Disorders
- Click Chemistry and Applications
- Rheumatoid Arthritis Research and Therapies
- Congenital gastrointestinal and neural anomalies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
HUN-REN Szegedi Biológiai Kutatóközpont
2021-2025
University of Szeged
2021-2024
Institute of Genetics
2023-2024
Vaccine-induced immunity is essential for controlling the COVID-19 pandemic. Data on humoral and cellular immunogenicity safety of different SARS-CoV-2 vaccines in patients with autoimmune rheumatic musculoskeletal diseases (RMDs) are limited.A single center observational study evaluated two-dose regimen BBIBP-CorV inactivated, Gam-COVID-Vac AZD1222 adenovirus-based, BNT162b2 mRNA-1273 mRNA-based RMDs (n = 89) compared healthy controls 74). Neutralizing anti-RBD (receptor binding domain)...
Introduction Tobacco smoking generates airway inflammation in chronic obstructive pulmonary disease (COPD), and its involvement the development of lung cancer is still among leading causes early death. Therefore, we aimed to have a better understanding disbalance immunoregulation inflammatory conditions smoker subjects with stable COPD (stCOPD), exacerbating (exCOPD), or non-small cell (NSCLC). Methods Smoker controls without illness were recruited as controls. Through extensive mapping...
Objectives Cell surface glycosylation can influence protein-protein interactions with particular relevance to changes in core fucosylation and terminal sialylation. Glycans are ligands for immune regulatory lectin families like galectins (Gals) or sialic acid immunoglobulin-like lectins (Siglecs). This study delves into the glycan alterations within subsets of systemic lupus erythematosus (SLE). Methods Evaluation binding affinities Galectin-1, Galectin-3, Siglec-1, Aleuria aurantia (AAL,...
Abstract Background Gelsectan ® is a formulation of xyloglucan (XG), pea protein, grape seed extract (PPGS) and xylo‐oligosaccharides (XOS). Our aim was to examine the effect on rectal sensitivity in an animal model, abdominal pain irritable bowel syndrome with diarrhoea (IBS‐D) subjects intestinal permeability both conditions. Methods Animals: Wistar rats received gavage XOS, XG + PPGS or as single dose for 7 days before partial restraint stress (PRS). Visceromotor response distension total...
Understanding leukemia-associated immunophenotypes (LAIP) could assist in the design of therapies to ameliorate patient benefits acute myeloid leukemia (AML). In our study, focusing on single-cell heterogeneity therapeutic resistance, flow cytometric immunophenotyping peripheral blood therapy-naive and follow-up AML patients versus age sex-matched healthy controls (HCs) was performed. The FACS panel consisted Viobility 405/520 Fixable Dye, Anti-human CD45, CD19, CD3, CD7, CD33, CD34, CD38,...
The effect of platinum-based chemotherapy (Chem.) and second- or multiple- line immune checkpoint PD-1 blocking therapy by Nivolumab Pembrolizumab (ICI) was assayed in the peripheral blood non-small cell lung cancer (NSCLC) patients.Flow cytometry used to detect NSCLC-related antigen binding IgG antibodies. Luminex MagPix multiplex bead-based cytokine/chemokine detecting system quantitatively measure 17 soluble markers plasma samples. Single-cell mass applied for immunophenotyping...
Abstract Background Obesity is a growing problem worldwide and major risk factor for many chronic diseases. The accumulation of adipose tissue leads to the release significant amounts pro-inflammatory cytokines adipokines, resulting in low-grade systemic inflammation. However, mechanisms behind development obesity-related diseases are not fully understood. Therefore, our study aimed investigate pathological changes inflammatory processes at level individual organs two different diet-induced...
Cervical carcinoma is one of the most frequent malignant gynecological cancers in women reproductive age. Because poor tolerability currently available chemotherapeutic agents, efforts have been focused on developing innovative molecules, including steroids, that exert antineoplastic effects with a better safety profile. In addition to their endocrine properties, certain estrogens exhibit additional biological activities, such as antiangiogenic and anticancer effects. Based previous studies,...
The present study aimed to characterize the antiproliferative and antimetastatic properties of two recently synthesized monoterpene-aminopyrimidine hybrids (1 2) on A2780 ovary cancer cells. Both agents exerted a more pronounced cell growth inhibitory action than reference agent cisplatin, as determined by MTT assay. Tumor selectivity was assessed using non-cancerous fibroblast Hybrids 1 2 induced changes in morphology membrane integrity cells, evidenced Hoechst 33258-propidium iodide...
Intratumoral heterogeneity (ITH) is responsible for the majority of difficulties encountered in treatment lung-cancer patients. Therefore, NSCLC cell lines and primary lung adenocarcinoma was investigated by single-cell mass cytometry (CyTOF). First, we studied frequent models, such as A549, H1975, H1650. The intra- inter-cell-line represented expression patterns 13 markers-namely GLUT1, MCT4, CA9, TMEM45A, CD66, CD274 (PD-L1), CD24, CD326 (EpCAM), pan-keratin, TRA-1-60, galectin-3,...
The advent of immunotherapy has revolutionized cancer treatments. However, the application immune checkpoint inhibitors may entail severe side effects, with risk therapeutic resistance. generation chimeric antigen receptor (CAR) T-cells or CAR-NK cells requires specialized molecular laboratories, is costly, and difficult to adapt rapidly growing number patients. To provide a simpler but effective therapy, whole-cell tumor vaccine protocol was established based on ultraviolet C...
The quantitative detection of radiation caused DNA double-strand breaks (DSB) by immunostained γ-H2AX foci using direct stochastic optical reconstruction microscopy (dSTORM) provides a deeper insight into the repair process at nanoscale in time-dependent manner. Glioblastoma (U251) cells were irradiated with 250 keV X-ray 0, 2, 5, 8 Gy dose levels. Cell cycle phase distribution and apoptosis U251 upon irradiation was assayed flow cytometry. We studied density, topology volume 3D confocal...
Vaccination against SARS-CoV-2 to prevent COVID-19 is highly recommended for immunocompromised patients with autoimmune rheumatic and musculoskeletal diseases (aiRMDs). Little known about the effect of booster vaccination or infection followed by previously completed two-dose in aiRMDs. We determined neutralizing anti-SARS-CoV-2 antibody levels applied flow cytometric immunophenotyping quantify reactive B- T-cell mediated immunity aiRMDs receiving homologous heterologous boosters acquired...
Vaccination has proven the potential to control COVID-19 pandemic worldwide. Although recent evidence suggests a poor humoral response against SARS-CoV-2 in vaccinated hematological disease (HD) patients, data on vaccination these patients is limited with comparison of mRNA-based, vector-based or inactivated virus-based vaccines.Forty-nine HD and 46 healthy controls (HCs) were enrolled who received two-doses complete BNT162b2, AZD1222, BBIBP-CorV, respectively. The antibodies reactive...
Abstract Here, we describe the synthesis and biologic activity evaluation of 20 novel synthetic marine sponge alkaloid analogues with 2‐amino‐1H‐imidazol (2‐AI) core. Cytotoxicity was tested on murine 4T1 breast cancer, A549 human lung HL‐60 myeloid leukemia cells by resazurin assay. A total 18 compounds showed cytotoxic effect cancer cell lines different potential. Viability healthy fibroblasts peripheral blood mononuclear upon treatment less hampered compared to supporting tumor specific...
Four diastereomers of 16-azidomethyl substituted 3-O-benzyl estradiol (1–4) and their two estrone analogs (16AABE 16BABE) were tested for antiproliferative properties against human gynecological cancer cell lines. The estrones selected additional experiments based on outstanding growth-inhibiting activities. Both compounds increased hypodiploid populations breast cells, 16AABE elicited cycle disturbance as evidenced by flow cytometry. substantially the rate tubulin polymerization in vitro....