A5102844438- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Immune cells in cancer
- Bladder and Urothelial Cancer Treatments
- Educational Robotics and Engineering
- Immunotherapy and Immune Responses
- interferon and immune responses
- RNA and protein synthesis mechanisms
- Bone Metabolism and Diseases
- Tryptophan and brain disorders
- Cancer Diagnosis and Treatment
- RNA regulation and disease
- CAR-T cell therapy research
- Pluripotent Stem Cells Research
- Diabetes and associated disorders
- Cancer Immunotherapy and Biomarkers
- Lymphatic System and Diseases
- Asthma and respiratory diseases
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Bone health and treatments
The University of Tokyo
2001-2024
Kyoto University
2020-2021
Yamaguchi University
2016
Abstract Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may cause off-target mutagenesis immunogenicity. Thus, a transient delivery system is needed for therapeutic genome editing applications. Here, we develop an extracellular nanovesicle-based ribonucleoprotein named NanoMEDIC by utilizing two distinct homing mechanisms. Chemical induced dimerization recruits Cas9 protein into nanovesicles, then RNA packaging signal self-cleaving riboswitches tether release...
Combined with CRISPR-Cas9 technology and single-stranded oligodeoxynucleotides (ssODNs), specific single-nucleotide alterations can be introduced into a targeted genomic locus in induced pluripotent stem cells (iPSCs); however, ssODN knockin frequency is low compared deletion induction. Although several Cas9 transduction methods have been reported, the biochemical behavior of nuclease mammalian yet to explored. Here, we investigated intrinsic cellular factors that affect cleavage activity...
Therapeutic cancer vaccines are designed to treat by boosting the endogenous immune system fight against cancer. In development of clinically effective vaccines, one most practical objectives is identify adjuvants that capable optimizing vaccine effects. this study, we explored potential polyinosinic–polycytidylic acid (poly(I:C)) and LAG ‐3‐Ig (soluble recombinant protein lymphocyte activation gene‐3 [ ‐3] extracellular domain fused with human IgG Fc region) as for P1A tumor antigen peptide...
Abstract The thymus is an organ required for T cell development and also eosinophil-rich organ; however, the nature function of thymic eosinophils remain unclear. Here, we characterized gene expression differentiation mechanism in mice. Thymic showed a distinct profile compared with other organ-resident eosinophils. number was controlled by medullary epithelial cells (mTECs). In Rag-deficient mice, unique signature lost but restored pre-T receptor signalling, which induces CD4+ CD8+...
Recent studies demonstrate that extracellular-released aminoacyl-tRNA synthetases (aaRSs) play unique roles in immune responses and diseases. This study aimed to understand the role of extracellular aaRSs pathogenesis rheumatoid arthritis (RA).Primary macrophages fibroblast-like synoviocytes were cultured with aaRSs. aaRS-induced cytokine production including IL-6 TNF-α was detected by ELISA. Transcriptomic features aaRS-stimulated examined using RNA-sequencing. Serum synovial fluid (SF)...
The decisions in the initial design stage make large impact upon all life cycle of product more than other stages. concept FOA (First Order Analysis) is proposed for stage, and framework connecting 'design' 'analysis' essential to achieve FOA. On top that, three important factors supporting system, that say simple, flexible gradual, are listed up this report. Then a system based on authors' developing 'Top-Down Oriented Design Support System' implemented. This embodies framework, some...