A5019263681- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Crop Yield and Soil Fertility
- Plant Molecular Biology Research
- Genetic and phenotypic traits in livestock
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Microbial Metabolites in Food Biotechnology
- Pancreatitis Pathology and Treatment
- Higher Education and Teaching Methods
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Pancreatic and Hepatic Oncology Research
- Polysaccharides and Plant Cell Walls
- Polysaccharides Composition and Applications
- Biofuel production and bioconversion
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- RNA modifications and cancer
- Plant Gene Expression Analysis
- Nutrition, Health and Food Behavior
- Bacteriophages and microbial interactions
- Banana Cultivation and Research
- Radiology practices and education
Guizhou University
2021-2023
Shaanxi Normal University
2007-2022
Zhengzhou Central Hospital
2022
Sichuan Agricultural University
2013-2021
Misión Biológica de Galicia
2019-2021
Gansu Provincial Maternal and Child Health Hospital
2019-2020
The Affiliated Yongchuan Hospital of Chongqing Medical University
2020
Chongqing Medical University
2020
First Affiliated Hospital of Fujian Medical University
2014-2017
Fujian Medical University
2014-2017
Starch synthesis is a key process that influences crop yield and quality, though little known about the regulation of this complex metabolic pathway. Here, we present identification ZmbZIP91 as candidate regulator starch via co-expression analysis in maize (Zea mays L.). was strongly associated with expression genes. Reverse tanscription–PCR (RT–PCR) RNA situ hybridization indicated highly expressed endosperm, less leaves. Particle bombardment-mediated transient endosperm leaf protoplasts...
Polygonatum sibiricum polysaccharides (PSPs) are used to improve immunity, alleviate dryness, promote the secretion of fluids, and quench thirst. However, PSP biosynthetic pathway is largely unknown. Understanding genetic background will help delineate that at molecular level so researchers can develop better conservation strategies. After comparing contents among several different P. germplasms, we selected two groups with largest contrasts in subjected them HiSeq2500 transcriptome...
Utilization of heterosis in maize could be critical breeding for boosting grain yield. However, the genetic architecture is not fully understood. To dissect basis yield-related traits and maize, 301 recombinant inbred lines derived from 08 to 641 × YE478 298 hybrids immortalized F2 (IF2) population were used map quantitative trait loci (QTLs) nine mid-parent heterosis.We observed 156 QTLs, 28 pairs with epistatic interaction, 10 significant QTL environment interactions hybrid mapping...
Abstract Maize is one of the three major crops worldwide based on its yield and quality. Starch crucial to both quality maize as it accounts more than 60% seed weight, structure influences crop. synthase I (SSI) contributes majority starch activity in endosperm. An in-depth understanding synthesis regulatory mechanism would provide opportunities for improving maize. In this study, ZmPLATZ2 , a plant AT-rich sequence zinc-binding protein (PLATZ) transcription factor related synthesis, was...
Emerging studies have reported that coatomer protein complex subunit β2 (COPB2) is overexpressed in several types of malignant tumor; however, to the best our knowledge, no regarding COPB2 gastric cancer been published thus far. Therefore, present study aimed determine significance and function cancer. expression cell lines was measured using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis. In addition, lentivirus‑short hairpin RNA (shRNA) (Lv‑shCOPB2)...
Abstract Background Short internodes contribute to plant dwarfism, which is exceedingly beneficial for crop production. However, the underlying mechanisms of internode elongation are complicated and have been not fully understood. Results Here, we report a maize dwarf mutant, dwarf2014 ( d2014 ), displays shortened lower internodes. Map-based cloning revealed that gene novel br2 allele with splicing variation, resulting in higher expression BR2-T02 instead normal BR2-T01 . Then, found...
High-yielding cultivars with high water use efficiency are a main target in maize breeding; yet, no comprehensive study about the genetic regulation of photosynthesis, or ranges variability for gas exchange-related parameters has been published. Here, large panel 731 inbred lines previously genotyped was evaluated to elucidate basis photosynthesis-related parameters, measured 15–30 days after silking, across three years. Large phenotypic and genotypic variations were observed this dramatic...
This study aimed to investigate the mutations in serine protease 1 gene (PRSS1) and imbalance between trypsin α1-antitrypsin patients with pancreatic cancer. Polymerase chain reaction (PCR) was performed amplify sequences of PRSS1 from 65 cancer 260 healthy controls, direct sequencing performed, clinical features were analyzed. In addition, enzyme-linked immunosorbent assay (ELISA) employed detect serum controls same period. Mutations found at promoter exon 3 That is, five had c.410 C > T...
The development of maize foundation parents is an important part genetics and breeding research, applying new genetic information to produce has been challenging. In this study, we focused on quantitative trait loci (QTLs) general combining ability (GCA) Ye478, a widely used parent in China. We developed three sets populations for QTL mapping analyze the GCA some agronomic traits. assessment 15 traits resulted detection 251 QTLs six tested environments, with 119 identified through joint...
MicroRNA (miRNA) is known to be involved in the pathological process of congenital heart disease (CHD), and nodal modulator1 (NOMO1) a critical determinant formation. The present study aims discover effect miR-33a-5p NOMO1 on CHD.Quantitative real-time polymerase chain reaction (qRT-PCR) was used detect expressions mimic or inhibitor overexpressed plasmid orNOMO1 knockdown. Human cardiomyocyte progenitor cells (hCMPCs) proliferation measured by cell counting kit-8 (CCK-8) at 24, 48 72 h....
To construct a restriction endonuclease based biosensor technology for PRSS1 genotyping. We designed thiol-modified hairpin probe where the neck has EcoRI recognition sites according to gene c.410 C>T (p.T137 M) mutation and it was fixed on gold electrode. Different charge generated by binding of MB phosphate groups DNA before after hybridization used distinguishing different genotypes quantity. This showed that novel sensor can better distinguish complementary sequence, single-base...
Gray leaf spot (GLS), caused by different species of Cercospora , is a fungal, non-soil-borne disease that causes serious reductions in maize yield worldwide. The identification major quantitative trait loci (QTLs) for GLS resistance essential developing marker-assisted selection strategies breeding. Previous research found significant difference ( P < 0.01) between T32 (highly resistant) and J51 susceptible) genotypes maize. Initial QTL analysis was conducted an F 2 : 3 population...
Objective: To develop a convenient; sensitive; accurate; and economical technique for genotyping of hepatitis B viruses (HBVs). Methods: The mercapto-modified B1; B2; C1; C2-specific probes consisted two each HBV genotype that served as double verification system. These were fixed on the surface No. 1; 2; 3; 4 gold electrodes; respectively; via Au-S bonds. Different charge generated by binding RuHex to phosphate groups DNA backbone before after hybridization was used distinguishing different...
Flowering-related traits in maize are affected by complex factors and important for the improvement of cropping systems zone. Quantitative trait loci (QTLs) detected using different materials methods usually vary. In present study, 266 (Zea mays) F2:3 families 301 recombinant inbred lines (RIL) derived from a cross between 08-641 (founding parent southeast China) Ye478 were evaluated four flowering-related traits, including days to tasseling (DTT), pollen shedding (DPS), silking (DTS),...
Objective: We aimed to study the expressions of miR-103a-3p and TRIM66 in prostate cancer (PCa) cells, explore direct target genes miR-103a-3p, analyze effects targeted regulation axis on docetaxel (DTX) resistance glycolysis PCa cells. Methods: Human normal cells were used detect their relationship. DTX-resistant (DR) established transfected with plasmids. The MTT assay, plate cloning wound healing Transwell assay cell viability, colony formation, migration, invasion, respectively. Cell was...