Soil contamination with heavy metals is a widespread problem, especially prominent on grounds lying in the vicinity of mines, smelters, and other industrial facilities. Many such areas are located Southern Poland; they polluted mainly Pb, Zn, Cd, or Cu, locally also Cr. As for now, little known about most bacterial species thriving soils even less core community--a set taxa common to soils. Therefore, we wanted answer question if could be found samples differing physicochemically...

Reaction of soil bacteria to drought and rewetting stress may depend on chemical properties. The objectives this study were test the reaction different bacterial phyla assess influence properties kind stress. samples taken at ten forest sites measured for pH contents organic C (Corg) total N (Nt), Zn, Cu, Pb. kept without water addition 20 – 30 °C 8 weeks subsequently rewetted achieve moisture equal 50 60 % their maximum water-holding capacity. Prior period 24 h after rewetting, structure...

Here we report the nucleotide sequence of pCTX-M3, a highly conjugative plasmid that is responsible for extensive spread gene coding CTX-M-3 extended-spectrum beta-lactamase in clinical populations family Enterobacteriaceae Poland. The belongs to IncL/M incompatibility group, 89,468 bp size, and carries 103 putative genes. Besides bla(CTX-M-3), it also bears bla(TEM-1), aacC2, armA genes, as well integronic aadA2, dfrA12, sul1, which altogether confer resistance majority beta-lactams...

Chronic inflammation and enteric infections are frequently associated with epithelial Na(+)/H(+) exchange (NHE) inhibition. Alterations in electrolyte transport mucosal pH may represent a key mechanism leading to changes the intestinal microbial composition. NHE3 expression is essential for maintenance of barrier function. NHE3(-/-) mice develop spontaneous distal chronic colitis highly susceptible dextran sulfate (DSS)-induced injury. Spontaneous reduced broad-spectrum antibiotics...

Black alder (Alnus glutinosa Gaertn.) belongs to dual mycorrhizal trees, forming ectomycorrhizal (EM) and arbuscular (AM) root structures, as well represents actinorrhizal plants that associate with nitrogen-fixing actinomycete Frankia sp. We hypothesized the unique ternary structure of symbionts can influence community other plant-associated microorganisms (bacterial fungal endophytes), particularly under seasonally changing salinity in A. roots. In our study we analyzed black bacterial...

Sugar beet is a highly salt-tolerant crop. However, its ability to withstand high salinity reduced compared sea beet, wild ancestor of all crops. The aim this study was investigate transcriptional patterns associated with physiological, cytological and biochemical mechanisms involved in salt response these closely related subspecies. Salt acclimation strategies were assessed plants subjected either gradually increasing levels (salt-stress) or excised leaves, exposed instantly (salt-shock)....

We examined Salicornia europaea, a nonmycorrhizal halophyte associated with specific and unique endophytic bacteria fungi. The microbial community structure was analyzed at two sites differing in salinization history (anthropogenic naturally saline site), contrasting seasons (spring fall) plant organs (shoots roots) via 16S rRNA internal transcribed spacer amplicon sequencing. observed distinct communities the sites, shoots roots, while season of no importance. bacterial less diverse shoot...

Plant litter decomposition is a process enabling biogeochemical cycles closing in ecosystems, and forests constitutes the largest part of this taking place terrestrial biomes. Microbial communities during were studied mainly with low-throughput techniques not allowing detailed insight, particularly into coniferous litter, as it more difficult to obtain high quality DNA required for analyses. Motivated by these problems, we analyzed archaeal, bacterial, eukaryotic at three stages: fresh, 3-...

The gut microbiota in patients with food allergy, and the skin atopic dermatitis differ from those of healthy people. We hypothesize that relationships may exist between allergies. aim this study was to determine possible relationship allergies, hence simultaneous analysis two compartments performed infants without allergic symptoms. Fifty-nine allergy and/or 28 children were enrolled study. evaluated using 16S rRNA gene amplicon sequencing. No significant differences α-diversity dermal or...

Plant colonization by microbes is an example of succession, with its distinct phases differing in community structure and diversity. This process needs to be studied improve bioinoculation strategies. Here, we show that, regardless bioinoculation, soil type plant genotype, bacteria colonize the rhizosphere tissues axenic beets two associated taproot development. Communities remained stable after five weeks growth soil. Time, genotype determined both endosphere. Inoculation changed structure,...

The aim of this paper was to develop a protocol for efficient storage artificial seeds Taraxacum pieninicum, critically endangered Asteraceae species. Storage under reduced light conditions or in the darkness tested on basis synthetic ability conversion and post-storage regrowth shoot tips. results indicated that synseeds obtained from tips T. pieninicum can be stored at 4 °C even 12 months without subculture. is stress factor during what manifested by numerous necrosis decreased shoots...

The extrachromosomal gene pool plays a significant role both in evolution and the environmental adaptation of bacteria. L. lactis subsp. IL594 strain contains seven plasmids, named pIL1 to pIL7, is parental plasmid-free IL1403, which one best characterized lactococcal strains LAB. Complete nucleotide sequences (6,382 bp), pIL2 (8,277 pIL3 (19,244 pIL4 (48,979), pIL5 (23,395), pIL6 (28,435 bp) pIL7 (28,546) were established deposited generally accessible database (GeneBank). Nine highly...

Abstract Constipation is a widespread problem in paediatric practice, affecting almost 30% of children. One the key causal factors constipation may be disturbances homeostasis gastrointestinal microbiome. The aim study was to determine whether oral and fecal microbiomes differ between children with without constipation. A total 91 over three years age were included study. Of these, 57 qualified group constipation, 34 without. saliva stool evaluated using 16S rRNA gene amplicon sequencing....

Intraterrestrial waters harbor microbial communities being extensively studied to understand processes underlying subsurface ecosystem functioning. This paper provides the results of an investigation on microbiomes unique, sulfidic associated with Upper Jurassic, Cretaceous, and Miocene sediments. We used high-throughput 16S rDNA amplicon sequencing reveal structure bacterial archaeal in water samples differing sulfide content (20-960 mg/dm3), salinity (1.3-3.2%), depth extraction (60-660 m...

Most bacteria are found either in marine or fresh waters and transitions between the two habitats rare, even though freshwater co-occur brackish habitats. Estuaries brackish, tideless seas could be where transition of phylotypes to conditions occurs. We tested this hypothesis Gulf Gdańsk (Baltic Sea) by comparing bacterial communities from different zones estuary, via pyrosequencing 16S rRNA amplicons. predicted existence a core microbiome (CM, set abundant OTUs present all samples)...

The child microbiome, including gut and skin communities, is shaped by a multitude of factors, breastfeeding one the most essential. Food allergy (FA) atopic dermatitis (AD) are among common diseases in pediatrics, with prevalence each up to 6% 20%, respectively. Therefore, we aimed at finding differences between fecal microbiomes FA AD patients context breastfeeding, means Illumina sequencing 16S rRNA gene fragment libraries amplified from total DNA isolated samples collected allergic...

Phosphorus (P) is an essential plant nutrient. Low availability of P in soil mainly caused by high content Fe 2 O 3 the clay fraction that binds to making it unavailable. Beneficial microbes, such as solubilizing microorganisms can increase available and improve growth productivity. In this study, we evaluated effects environmental conditions (climate, parameters), genotype, level association (rhizosphere or endophytic root organism) on abundance diversity phosphorus a Salix production...

Escherichia coli isolates recovered from patients during a clonal outbreak in Warsaw, Poland, hospital 1997 produced different levels of an extended-spectrum beta-lactamase (ESBL) the SHV type. The hyperproduction correlated with multiplication ESBL gene copies within plasmid. Here, we present complete nucleotide sequence plasmid p1658/97 carried by outbreak. is 125,491 bp and shows mosaic structure which all modules constituting core are homologous to those found plasmids F R100 separated...

Salicornia europaea, a succulent obligatory halophyte is the most salt-tolerant plant species in world. It survives salt concentrations of more than 1 M. Therefore, it suitable model to identify genes involved tolerance mechanisms that can be used for improvement crops. The changes plant's gene expression response abiotic stresses may depend on factors like soil conditions at site, seasonality, etc. To date, experiments were performed study S. europaea only under controlled conditions....

Plasmid conjugative transfer systems comprise type IV secretion (T4SS) coupled to DNA processing and replication. The T4SSs are divided into two phylogenetic subfamilies, namely, IVA IVB, or on the basis of phylogeny VirB4 ATPase, eight groups. conjugation system IncM group plasmid pCTX-M3, from Citrobacter freundii, is classified in IVB subfamily MPFI group, as IncI1 plasmids. Although majority genes plasmids display conserved synteny, there several differences. Here, we present a deletion...