A5091663223- Mosquito-borne diseases and control
- Malaria Research and Control
- Insect symbiosis and bacterial influences
- interferon and immune responses
- Animal Virus Infections Studies
- Protist diversity and phylogeny
- Viral Infections and Vectors
- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Evolution and Genetic Dynamics
- Microtubule and mitosis dynamics
- Viral Infections and Immunology Research
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Insect and Pesticide Research
Yale University
2023-2025
National Institutes of Health
2021
National Heart Lung and Blood Institute
2021
Fred Hutch Cancer Center
2020
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2020
Cancer Research Center
2020
Abstract Dengue is the most prevalent mosquito-borne viral disease in humans, and cases are continuing to rise globally. In particular, islands Caribbean have experienced more frequent outbreaks, all four dengue virus (DENV) serotypes been reported region, leading hyperendemicity increased rates of severe disease. However, there significant variability regarding surveillance reporting between islands, making it difficult obtain an accurate understanding epidemiological patterns Caribbean. To...
Abstract Background The increasing burden of dengue virus on public health due to more explosive and frequent outbreaks highlights the need for improved surveillance control. Genomic not only provides important insights into emergence spread genetically diverse serotypes genotypes, but it is also critical monitor effectiveness newly implemented control strategies. Here, we present DengueSeq, an amplicon sequencing protocol, which enables whole-genome all four serotypes. Results We developed...
Dengue virus (DENV) is a mosquito-borne that poses continued and increasing threat to public health. A promising strategy mitigate the burden of DENV introgression virus-inhibiting Wolbachia pipientis bacterium into Aedes aegypti populations in field. While previous studies on -mediated inhibition have typically assessed viral replication following single bloodmeal, main vector DENV, Ae. , feeds frequently, often biting multiple hosts per gonotrophic cycle promptly attempting refeed egg...
Host antiviral proteins engage in evolutionary arms races with viruses, which both sides rapidly evolve at interaction interfaces to gain or evade immune defense. For example, primate TRIM5α uses its evolving ‘v1’ loop bind retroviral capsids, and single mutations this can dramatically improve restriction. However, it is unknown whether such gains of viral restriction are rare, if they incur loss pre-existing function against other viruses. Using deep mutational scanning, we comprehensively...
Abstract Dengue is the most prevalent mosquito-borne viral disease in humans, and cases are continuing to rise globally. In particular, islands Caribbean have experienced more frequent outbreaks, all four dengue virus (DENV) serotypes been reported region, leading hyperendemicity increased rates of severe disease. However, there significant variability regarding surveillance reporting between islands, making it difficult obtain an accurate understanding epidemiological patterns Caribbean. To...
The increasing burden of dengue virus on public health due to more explosive and frequent outbreaks highlights the need for improved surveillance control. Genomic not only provides important insights into emergence spread genetically diverse serotypes genotypes, but it is also critical monitor effectiveness newly implemented control strategies. Here, we present DengueSeq, an amplicon sequencing protocol, which enables whole-genome all four serotypes.
Abstract Diverse mammalian species display susceptibility to SARS-CoV-2. Potential SARS-CoV-2 spillback into rodents is understudied despite their host role for numerous zoonoses and human proximity. We assessed exposure infection among white-footed mice ( Peromyscus leucopus ) in Connecticut, USA. observed 1% (6/540) wild-type neutralizing antibody seroprevalence 2020–2022 residential with no cross-neutralization of variants. detected infections via RT-qPCR, but identified non-SARS-CoV-2...
Abstract Diverse mammalian species display susceptibility to and infection with SARS-CoV-2. Potential SARS-CoV-2 spillback into rodents is understudied despite their host role for numerous zoonoses human proximity. We assessed exposure among white-footed mice ( Peromyscus leucopus ) in Connecticut, USA. observed 1% (6/540) wild-type neutralizing antibody seroprevalence 2020-2022 residential no cross-neutralization of variants. detected infections via RT-qPCR, but identified non-SARS-CoV-2...
ABSTRACT Host antiviral proteins engage in evolutionary arms races with viruses, which both sides rapidly evolve at interaction interfaces to gain or evade immune defense. For example, primate TRIM5α uses its evolving “v1” loop bind retroviral capsids, and single mutations this can dramatically improve restriction. However, it is unknown whether such gains of viral restriction are rare, if they incur loss pre-existing function against other viruses. Using deep mutational scanning, we...
Abstract Centrosomes are multi-protein complexes that function as the major microtubule organizing center (MTOC) for cell. While centrosomes play tissue-specific MTOC functions, little is known about how particular centrosome proteins regulated across cell types to achieve these different functions. To investigate this type-specific diversity, we searched gene duplications of genes in Drosophila lineage with aim identifying where each copy evolved specialized Through depth functional...