A5017068832- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- Insect and Pesticide Research
- Health and Conflict Studies
- Studies on Chitinases and Chitosanases
- Mesenchymal stem cell research
- Animal Disease Management and Epidemiology
- T-cell and Retrovirus Studies
- Insect Resistance and Genetics
- Virus-based gene therapy research
- Parvovirus B19 Infection Studies
- Parasitic Infections and Diagnostics
- Parasites and Host Interactions
- Spaceflight effects on biology
- Cytomegalovirus and herpesvirus research
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Child Abuse and Trauma
- Muscle Physiology and Disorders
- Maternal Mental Health During Pregnancy and Postpartum
- Syphilis Diagnosis and Treatment
Connecticut Agricultural Experiment Station
2022-2025
Pennsylvania State University
2015-2023
Center for Disease Dynamics, Economics & Policy
2018-2022
University Hospitals Bristol NHS Foundation Trust
2021
University of Arizona
2009
Michigan State University
1987-2001
Significance Factors influencing Wolbachia transfer into new species remain poorly understood. This is important as can influence speciation and being developed a novel arthropod-borne disease control approach. We show the native microbiota of Anopheles impede vertical transmission . Antibiotic microbiome perturbation enables in two species. Mosquitoes with altered microbiomes do not exhibit blood meal-induced mortality associated infection, suggesting that mosquitoes are killed by...
Muscle regeneration involves the coordination of myogenesis and revascularization to restore proper muscle function. Myogenesis is driven by resident stem cells termed satellite (SC), whereas angiogenesis arises from endothelial perivascular preexisting vascular segments collateral vasculature. Communication between myogenic angiogenic seems plausible, especially given number growth factors produced SC. To characterize these interactions, we developed an in vitro coculture model composed rat...
Although mosquitoes are major transmission vectors for pathogenic arboviruses, viral infection has little impact on mosquito health. This immunity is caused in part by RNA interference (RNAi) pathways that generate antiviral small interfering RNAs (siRNAs) and Piwi-interacting (piRNAs). RNAi also maintains genome integrity potently repressing transposon activity the germline soma. However, regulatory have not been systematically examined together mosquitoes. Therefore, we developed an...
Arboviruses can emerge rapidly and cause explosive epidemics of severe disease. Some the most epidemiologically important arboviruses, including dengue virus (DENV), Zika (ZIKV), Chikungunya (CHIKV) yellow fever (YFV), are transmitted by Aedes mosquitoes, notably aegypti albopictus. After a mosquito blood feeds on an infected host, enters midgut infects epithelium. The must then overcome series barriers before reaching saliva being to new host. escape from (known as barrier; MEB), which is...
Dengue virus (DENV) is a mosquito-borne that poses continued and increasing threat to public health. A promising strategy mitigate the burden of DENV introgression virus-inhibiting Wolbachia pipientis bacterium into Aedes aegypti populations in field. While previous studies on -mediated inhibition have typically assessed viral replication following single bloodmeal, main vector DENV, Ae. , feeds frequently, often biting multiple hosts per gonotrophic cycle promptly attempting refeed egg...
Aedes aegypti is a highly efficient vector for numerous pathogenic arboviruses including dengue virus (DENV), Zika virus, and yellow fever virus. This efficiency can in part be attributed to their frequent feeding behavior. We previously found that acquisition of second, full, non-infectious blood meal could accelerate dissemination within the mosquito by temporarily compromising midgut basal lamina integrity; however, wild, mosquitoes are often interrupted during only acquire partial or...
Mosquito-borne viruses cause more than 400 million annual infections and place over half of the world's population at risk. Despite this importance, mechanisms by which arboviruses infect mosquito host disseminate to tissues required for transmission are not well understood. Here, we provide evidence that immune cells, known as hemocytes, play an integral role in dissemination dengue virus (DENV) Zika (ZIKV)
Abstract AgDNV is a powerful gene transduction tool and potential biological control agent for Anopheles mosquitoes. Using GFP reporter virus system, we investigated host range specificity in four arthropod cell lines (derived from An. gambiae , Aedes albopictus Drosophila melanogaster ) six mosquito species 3 genera ( arabiensis stephensi Ae. aegypti Culex tarsalis ). In vitro efficient viral invasion, replication expression was only observed MOS55 cells. vivo high levels of were...
Recent studies demonstrate that insect-specific viruses can influence the ability of their mosquito hosts to become infected with and transmit arboviruses medical veterinary importance. The aim this study was evaluate interactions between Anopheles gambiae densovirus (AgDNV) (Parvoviridae) (a benign virus infects An. mosquitoes) Mayaro (MAYV) (Togaviridae) (an emerging human pathogen be transmitted by gambiae) in both insect cell culture mosquitoes.For vitro studies, Mos55 cells or...
Flaviviruses are arthropod-borne (arbo)viruses which can emerge rapidly and cause explosive epidemics of severe disease. Some the most epidemiologically important flaviviruses, including dengue virus (DENV), Zika (ZIKV) yellow fever (YFV), transmitted by
Anopheles gambiae densovirus (AgDNV) is an insect-specific, single-stranded DNA virus that infects An. sensu stricto (s.s.), the major mosquito species responsible for transmitting malaria parasites throughout sub-Saharan Africa. AgDNV a benign very specific to its host and therefore has potential serve as vector control tool via paratransgenesis (genetic modification of symbionts) limit transmission human pathogens. Prior being engineered into tool, natural dynamics between mosquitoes needs...
Anopheles gambiae densovirus (AgDNV) is a highly species specific parvovirus that reaches high titers in adult mosquitoes with few transcriptomic effects and no significant fitness effects. Given these characteristics, AgDNV has been proposed as viral vector for basic research mosquito control. Previous work created an co-expression system wild-type helper plasmid transducing enhanced green fluorescent protein (EGFP) expressing can be used to cotransfect cells generate infectious recombinant...
Autophagy is a critical modulator of pathogen invasion response in vertebrates and invertebrates. However, how it affects mosquito-borne viral pathogens that significantly burden public health remains underexplored. To address this gap, we use genetic approach to activate macroautophagy/autophagy the yellow fever mosquito (Aedes aegypti), infected with recombinant Sindbis virus (SINV) expressing an autophagy activator. We first demonstrate 17-amino acid peptide derived from Ae. aegypti...
ABSTRACT Although mosquitoes are major transmission vectors for pathogenic arboviruses, viral infection has little impact on mosquito health. This immunity is due in part to RNA interference (RNAi) pathways that generate antiviral small interfering RNAs (siRNAs) and Piwi-interacting (piRNAs). RNAi also maintains genome integrity by potently repressing transposon activity the germline soma. However, regulatory have not been systematically examined together mosquitoes. Therefore, we developed...