A5022334203- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Hematopoietic Stem Cell Transplantation
- Pediatric Hepatobiliary Diseases and Treatments
- Cytomegalovirus and herpesvirus research
- Hernia repair and management
- Multiple Myeloma Research and Treatments
- Organ Transplantation Techniques and Outcomes
- Platelet Disorders and Treatments
- Cavitation Phenomena in Pumps
- Immunotherapy and Immune Responses
- Intraperitoneal and Appendiceal Malignancies
- Blood donation and transfusion practices
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Bioinformatics and Genomic Networks
- Blood transfusion and management
- Evolution and Genetic Dynamics
- Gallbladder and Bile Duct Disorders
- Hydraulic and Pneumatic Systems
- Renin-Angiotensin System Studies
- Nutrition, Genetics, and Disease
Kyushu University
1994-2025
Kyushu University Hospital
2021-2024
Sendai Shakai Hoken Hospital
2012-2023
Japan Community Healthcare Organization
2023
Terumo (Japan)
2022
Kinki Central Hospital
2018-2020
Sendai Kousei Hospital
1999-2020
Mie University Hospital
2016
Mie University
2016
Kurume University
2012
Summary We investigated the role of monocyte/macrophage‐activating cytokines in pathogenesis haemophagocytic lymphohistiocytosis (HLH) 21 adult patients. Sera from patients with active HLH contained extremely high levels macrophage colony‐stimulating factor (M‐CSF) and interferon‐γ (IFN‐γ). These returned to almost normal during remission. Neither interleukin‐4 nor granulocyte/macrophage could be detected. Active sera also concentrations inflammatory monokines, such as interleukin‐6 (IL‐6)...
Angiotensin-converting enzyme (ACE) 2, a newly emerging component of the renin-angiotensin system, is presumed to be counterregulator against ACE in generating and degrading angiotensin II. It remains elucidated how mRNA levels these two genes are quantitatively regulated kidney also what kind clinicopathological characteristics could influence gene expressions humans. Seventy-eight cases biopsy-proven renal conditions were examined detail. Total RNA from small part each cortical biopsy...
A technique for the rapid detection of cytomegalovirus (CMV) antigen‐positive blood leucocytes (CMV antigenaemia) was evaluated in 15 marrow transplant patients as a means diagnosis and monitoring CMV‐associated disease. CMV antigenaemia determined by direct immunoperoxidase staining with peroxidase‐labelled monoclonal antibody, HRP‐C7, which binds an immediate‐early antigen human CMV. occurred 7/15 (47%) initially detected between 4 6 weeks after transplantation. diseases developed 3/15...
OBJECTIVE—Recent studies have proved that blockade of the renin-angiotensin system (RAS) retards progression diabetic nephropathy, whereas hyporeninemia is known as a typical state in subjects. The purpose this study to determine whether expression levels RAS differ between nondiabetic and renal tissues with accurate quantitative method. RESEARCH DESIGN AND METHODS—Subjects were 66 8 patients biopsy-proven diseases. eight subjects suffered from type 2 diabetes overt proteinuria. Renal...
Summary Cord blood transplantation (CBT) is a valuable donor source for patients without human leukocyte antigen (HLA)‐matched donors. While CBT has lower risk of graft‐versus‐host disease and requires less stringent histocompatibility, it associated with higher transplantation‐related mortality (TRM) compared to other sources. We hypothesized that assessing the immunogenicity mismatched HLA could reveal non‐permissive mismatches contributing increased TRM. retrospectively analysed 1498...
A cDNA clone, designated JTW15, has been isolated and found to encode a novel, divergent class I HLA gene transcript. The clone was identified by its ability hybridize with pan-HLA nucleotide probe while failing bind probes specific for the 3' untranslated exons of HLA-A, -B, -C genes. 1665 nucleotides in length, represents full length copy an approximately 1.7-kb mRNA detected Northern blot analyses. Comparison derived amino acid sequence those other proteins showed JTW15 be most so far,...
We investigated a dose-escalation effect of G-CSF (5, 10, and 15 μg/kg) on mobilization committed primitive hemopoietic progenitor cells, including CFU-GM, BFU-E, long-term culture-initiating cells (LTC-IC) in addition to CD34+ yields PBSC harvests obtained by leukapheresis healthy adult donors. Results indicate that the these is both dose time dependent. Despite very small number donors studied, it estimated from our data sufficient for allogeneic transplant (PBSCT) could be collected using...
Prior studies have shown that thymocytes, unlike peripheral T cells, do not proliferate in response to mitogenic combinations of anti-CD2 mAbs. The present study demonstrated stimulation by a combination (9-1 plus 9.6) with anti-CD28 induced vigorous thymocyte proliferation the absence exogenous IL-2. This was IL-2 dependent as complete inhibition using anti-IL-2-R Induction IL-2-R transcripts detected thymocytes stimulated antibody alone or antibody. However, induction observed only...
OBJECTIVE Recent studies have proven the favorable effects of angiotensin receptor blockers (ARBs) on cardiovascular and renal disorders. However, determinants response to ARBs remain unclear. We substantiated hypothesis that genetic variants renin-angiotensin system (RAS) significant impacts ARBs. RESEARCH DESIGN AND METHODS Subjects comprised 231 consecutively enrolled hypertensive individuals including 45 type 2 diabetic subjects. Five RAS, i.e., renin (REN) C-5312T, ACE...
The velocity fluctuations in the boundary layer around separation point and near-wake of a cylinder were measured at Re = 3.7 × 104 ∼2.8 105. All phenomena found seem to be interpreted by (1) oscillation fore aft point, (2) transverse separated shear layer, (3) temporary immersion ``tongue'' potential flow (4) plane axis wake vortex.
Anaphylatoxins generated during storage of platelet concentrates (PCs) may potentially have side effects on transfusion. We evaluated the anaphylatoxin-scavenging abilities white blood cell reduction filters. Among commercially available filters for PCs, one made with polyester fiber (PL50) dramatically adsorbed C3a and C4a anaphylatoxins to respective mean level 1,721-208 ng/ml 1,240-141 in 3-day-old PCs. were measured as native des Arg form each complement by radioimmunoassay. supernatant...
We have analysed the expression of HLA-G in 40 leukaemia samples various subtypes [seven cases acute lymphoblastic (ALL), 28 myelogenous (AML), three chronic (CML) and two lymphocytic (CLL)] by flow cytometry using HLA-G-specific monoclonal antibody. No expressed without incubation with interferon (IFN)-gamma. However, six out (21%) AML upon IFN-gamma. These derived from one seven M2, eight M4 five M5. The results indicated that cells, especially myelomonocytic samples, are capable...
In order to further clarify the diversity of HLA‐B15 antigens and correspondence serological types with alleles in Asians, we screened various B15 splits by means a polymerase chain reaction‐single strand conformation polymorphism (PCR‐SSCP) method. Subsequently, genes encoding variants were sequenced. Two novel alleles, B*1528 B*1529, identified: nucleotide sequence former contained single‐base substitution at position 263 exon 2 as compared that B*1501 allele, which results an amino acid...
Human cytomegalovirus glycoprotein B (gB) is synthesized as a 105-kDa nonglycosylated polypeptide and cotranslationally modified by addition of N-linked oligosaccharides to 160-kDa precursor in the endoplasmic reticulum (ER). It then transported Golgi complex, where it endoproteolytically cleaved form disulfide-linked mature gp55-116 complex. Pulse-chase experiments demonstrate that gB was transiently associated with calnexin, membrane-bound chaperone, ER. The association maximal immediately...
One of the HLA class II genes, HLA-DQA1, was typed from 290 unrelated healthy Japanese using oligonucleotide typing method. The HLA-DQA1 gene enzymatically amplified and by dot-blot hybridizations with 10 sequence-specific probes labeled nonradioactively. Using this method, genotype theoretically classified into 36 genotypes: 8 homozygous 28 heterozygous ones. Actually, 26 genotypes were observed in present study, frequency each allele calculated. numbers accordance expected under...
The underlying molecular basis for familial thrombocythaemia (FT), an extremely rare form of primary which occurs in autosomal dominant manner, is currently unknown. We have investigated a family with FT and clarified whether we could detect alteration(s) the genes coding c‐mpl its ligand, thrombopoietin (TPO). There was no difference platelet mRNA expression levels between affected non‐affected individuals family. Nucleotide sequence analysis cDNA proband revealed abnormality. identified...
Human cytomegalovirus (HCMV) infection results in a marked reduction the surface expression of class I major histocompatibility complex antigens on host cells, which is thought to be one means for HCMV evade immune system. To clarify precise mechanism(s) this phenomenon, we investigated fate heavy chain HCMV-infected human embryonic lung fibroblasts (HEL) by pulse-chase analysis and immunocytochemical techniques. In HEL, was synthesized at an increasing rate. However, instead being...