A5012651609- Amino Acid Enzymes and Metabolism
- Pancreatic function and diabetes
- Diet and metabolism studies
- Epigenetics and DNA Methylation
- Metabolism and Genetic Disorders
- Toxoplasma gondii Research Studies
- Diabetes and associated disorders
- Erythrocyte Function and Pathophysiology
- Polyamine Metabolism and Applications
- Mass Spectrometry Techniques and Applications
- Diabetes Treatment and Management
- Blood groups and transfusion
- Global Maternal and Child Health
- Pregnancy-related medical research
- Pregnancy and preeclampsia studies
- Health Policy Implementation Science
- Assisted Reproductive Technology and Twin Pregnancy
- Preterm Birth and Chorioamnionitis
- Urinary Tract Infections Management
- Antibiotic Resistance in Bacteria
- Atmospheric Ozone and Climate
- Atmospheric aerosols and clouds
- Maternal and Perinatal Health Interventions
- Liver Disease Diagnosis and Treatment
- Metabolism, Diabetes, and Cancer
Australian National University
2018-2025
National Institute of Health Sciences
2023
University of Gujrat
2022
Lady Reading Hospital
2020-2021
Holy Family Hospital
2020
Abstract Homeostasis is one of the fundamental concepts in physiology. Despite remarkable progress our molecular understanding amino acid transport, metabolism and signaling, it remains unclear by what mechanisms cytosolic concentrations are maintained. We propose that transporters primary determinants intracellular levels. show a cell’s endowment with can be deconvoluted experimentally used this data to computationally simulate translocation across plasma membrane. Transport simulation...
Lack of B 0 AT1 (SLC6A19) partially protects mice against the onset non-alcoholic steatohepatitis (NASH). To achieve a similar outcome through pharmacological treatment, we improved previously identified inhibitors by medicinal chemistry and second generation high through-put screening. Modified diarylmethine compounds inhibited with IC 50 values ranging from 8–90 μM. A was derived high-throughput screening showed higher affinity (IC 1–15 μM) strong selectivity amino acid transporters...
Abstract Context The obesity epidemic parallels an increasing type 1 diabetes incidence, such that westernized diets, containing high fat, sugar and/or protein, through inducing nutrient-induced islet beta-cell stress, have been proposed as contributing factors. broad-spectrum neutral amino acid transporter (B0AT1), encoded by Slc6a19, is the major acids in intestine and kidney. B0AT1 deficiency C567Bl/6J mice, causes aminoaciduria, lowers insulinemia improves glucose tolerance. Objective We...
Amino acid transporters play a vital role in metabolism and nutrient signaling pathways. Typically, transport activity is investigated using single substrates competing amounts of other amino acids. We used GC-MS LC-MS for metabolic screening Xenopus laevis oocytes expressing various human incubated complex media to establish their comprehensive substrate profiles. For most transporters, selectivity matched reported However, we could not detect substantial accumulation cationic acids by...
Dietary protein restriction has beneficial impacts on metabolic health. B0AT1 (SLC6A19) is the major transporter of neutral amino acids at intestinal epithelia and absorbs bulk diet-derived from lumen. It also reabsorbs in renal proximal tubules. Mice lacking show cellular outcomes restriction, such as high FGF21 levels low mTORC1 activity. Moreover, they have improved glucose homeostasis resist diet-induced obesity. In this study, we investigated relationship between dietary intake C57Bl6/J...
In humans, ∼0.1% to 0.3% of circulating red blood cells (RBCs) are present as platelet-RBC (P-RBC) complexes, and it is 1% 2% in mice. Excessive P-RBC complexes found diseases that compromise RBC health (eg, sickle cell disease malaria) contribute pathogenesis. However, the physiological role healthy unknown. As a result damage accumulated over their lifetime, RBCs nearing senescence exhibit molecular changes akin those platelet-binding malaria. Therefore, we hypothesized targets for...
Recent studies have established that dietary protein restriction improves metabolic health and glucose homeostasis. SLC6A19 (B0AT1) is the major neutral amino acid transporter in intestine carries out bulk of absorption from diet. Mice lacking show signs restriction, improved tolerance, are protected diet-induced obesity. Pharmacological blockage this could be used to induce treat diseases such as type 2 diabetes. A few novel inhibitors recently been identified using vitro compound...
Intracellular parasites of the phylum Apicomplexa are dependent on scavenging essential amino acids from their hosts. We previously identified a large family apicomplexan-specific plasma membrane-localized acid transporters, ApiATs, and showed that Toxoplasma gondii transporter Tg ApiAT1 functions in selective uptake arginine. is for parasite virulence, but dispensable growth medium containing high concentrations arginine, indicating presence at least one other arginine transporter. Here we...
High protein feeding has been shown to accelerate the development of type 1 diabetes in female non-obese diabetic (NOD) mice. Here, we investigated whether reducing systemic amino acid availability via knockout Slc6a19 gene encoding system B(0) neutral transporter AT1 would reduce incidence or delay onset NOD deficient mice were generated using CRISPR-Cas9 which resulted marked aminoaciduria. The by week 30 was 59.5% (22/37) and 69.0% (20/29) NOD.Slc6a19+/+ NOD.Slc6a19−/− mice, respectively...
Abstract Homeostasis is one of the fundamental concepts in physiology. Despite remarkable progress our molecular understanding amino acid transport, metabolism and signalling, it remains unclear by what mechanisms cytosolic concentrations are maintained. We propose that transporters primary determinants intracellular levels. show a cell’s endowment with can be deconvoluted logical series experiments. This was used to computationally simulate translocation across plasma membrane. For two...
In Pakistan, although coverage of Maternal, Newborn, and Child Health (MNCH) services has increased, the attributable disease burden remains high, indicating quality these suboptimal. To address this gap, challenges associated with implementation MNCH will need to be addressed effective use various guidelines supported, evaluated, continuously improved. Even though application field science practice in low- middle-income settings been limited, it is our belief, based on experience described...
Objective: To assess the maternal outcome of primigravida patient with term pregnancy engaged versus unengaged foetal head at onset labour Design Study: It was a cohort study. Study Settings: The study conducted Department Obstetrics & Gynecology Rashid Latif Medical College, Lahore from January 2021 to June 2021. Material and Methods: This involved 160 (as per dating scan) aged between 18-35 years who were divided into cases (women fetal head) controls (those head). Outcome variable...
Abstract Intracellular parasites of the phylum Apicomplexa are dependent on scavenging essential amino acids from their hosts. We previously identified a large family apicomplexan-specific plasma membrane-localized acid transporters, ApiATs, and showed that Toxoplasma gondii transporter Tg ApiAT1 functions in selective uptake arginine. is for parasite virulence, but dispensable growth medium containing high concentrations arginine, indicating presence at least one other arginine transporter....
Background: Escherichia coliresistance to ceftriaxone in UTIs is an emerging health problem.Our objectives were determine prevalence, distribution and determinants of E. adult indoor UTI population District Peshawar, Pakistan. Materials & Methods:This cross-sectional study was conducted Department Medicine, Khyber Teaching Hospital, Pakistan from 1st January 2017 30th June 2017. 380 cases selected at riskconsecutively.Sex age groups demographic, while presence coli resistance research...
Objective: To determine the mean prolongation of pregnancy via human Chorionic gonadotropin (H.C.G) in patients presenting with preterm labor. Materials and methods: This descriptive case study was conducted obstetrics gynecology department, Women children teaching hospital Dera Ismail khan from October 21, 2019, to April 20, 2020. Result: A total 123 pregnant women labor were included study. Average age 25.44 years+4.70sd range 16-35 years. Prolongation by chorionic (HCG) more than 30 days....
Objective: To determine the frequency of stillbirths in obese pregnant women. Design Study: It’s a descriptive cross-sectional study. Study Settings: This study was carried out at Department Obstetrics and Gynecology, Lady Reading Hospital Peshawar from January 2018 to July 2018. Material Methods: In this total 289 patients were observed. Detailed history taken each patient, period gestation calculated 1st trimester scan, conducted by person having least 2 years post-graduate training...