A5068418882- Lipid metabolism and biosynthesis
- Metalloenzymes and iron-sulfur proteins
- Electrocatalysts for Energy Conversion
- Silk-based biomaterials and applications
- TGF-β signaling in diseases
- Ammonia Synthesis and Nitrogen Reduction
- Insect and Arachnid Ecology and Behavior
- Plant biochemistry and biosynthesis
- Neurobiology and Insect Physiology Research
- Legume Nitrogen Fixing Symbiosis
- Growth Hormone and Insulin-like Growth Factors
- Meat and Animal Product Quality
- Monoclonal and Polyclonal Antibodies Research
- Escherichia coli research studies
- Metabolomics and Mass Spectrometry Studies
- Bone Metabolism and Diseases
- Sunflower and Safflower Cultivation
- Plant nutrient uptake and metabolism
- Bacteriophages and microbial interactions
- Peanut Plant Research Studies
- Connective Tissue Growth Factor Research
- thermodynamics and calorimetric analyses
- Advanced Proteomics Techniques and Applications
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
Commonwealth Scientific and Industrial Research Organisation
2007-2024
CSIRO Land and Water
2015-2023
Prefectural University of Hiroshima
2021
Health Sciences and Nutrition
2017-2020
Agriculture and Food
2019
The University of Tokyo
2018
Ecosystem Sciences
2012-2013
Tokyo Dental College
2012-2013
ACT Government
2012
Australian National University
2001-2003
Synthesis and accumulation of the storage lipid triacylglycerol in vegetative plant tissues has emerged as a promising strategy to meet world's future need for vegetable oil. Sorghum (Sorghum bicolor) is particularly attractive target crop given its high biomass, drought resistance C4 photosynthesis. While oilseed-like levels have been engineered C3 model tobacco, progress monocot crops lagging behind. In this study, we report sorghum leaf between 3 8.4% on dry weight basis depending...
Summary Vegetable oils extracted from oilseeds are an important component of foods, but also used in a range high value oleochemical applications. Despite being biodegradable, nontoxic and renewable current plant suffer the presence residual polyunsaturated fatty acids that prone to free radical formation limit their oxidative stability, consequently shelf life functionality. Many decades breeding have been successful raising oleic content ~90%, come at expense overall field performance,...
Plant roots release a variety of organic compounds into the soil which alter physical, chemical and biological properties rhizosphere. Root exudates are technically challenging to measure in because difficult access can be bound by minerals or consumed microorganisms. Exudates easier with hydroponically-grown plants but, even here, simple such as sugars acids rapidly assimilated Sterile hydroponic systems avoid this shortcoming but it is very maintain sterility for long periods especially...
Summary Engineering high biomass plants that produce oil (triacylglycerol or TAG) in vegetative rather than seed‐related tissues could help meet our growing demand for plant oil. Several studies have already demonstrated the potential of this approach by creating transgenic crop and model accumulate TAG their leaves stems. However, synthesis may compete with other important carbon energy reserves, including carbohydrate production, thereby limit growth. The aims study were thus: first, to...
Amino acid transporters play a vital role in metabolism and nutrient signaling pathways. Typically, transport activity is investigated using single substrates competing amounts of other amino acids. We used GC-MS LC-MS for metabolic screening Xenopus laevis oocytes expressing various human incubated complex media to establish their comprehensive substrate profiles. For most transporters, selectivity matched reported However, we could not detect substantial accumulation cationic acids by...
Virus-like particles (VLPs) derived from bacteriophage P22 have been explored as biomimetic catalytic compartments. In vivo colocalization of enzymes within VLPs uses sequential fusion to the scaffold protein, resulting in equimolar concentrations enzyme monomers. However, control over stoichiometry, which has shown influence pathway flux, is key realizing full potential artificial metabolons. We present a tunable strategy for stoichiometric co-encapsulation cargo proteins, verified...
Collagen is ubiquitous throughout the animal kingdom, where it comprises some 28 diverse molecules that form extracellular matrix within organisms. In 1960s, an extracorporeal collagen forms cocoon of a small group hymenopteran insects was postulated. Here we categorically demonstrate larvae sawfly species produce silk from three proteins. The native proteins do not contain hydroxyproline, post translational modification normally considered characteristic collagens. function as silks...
Significance Engineering nitrogenase in plants may help alleviate economic and environmental issues due to the use of nitrogen fertilizer. Mitochondria have shown promise supporting function nitrogenase, including electron donation metallocluster assembly. Despite these successes, formation catalytic unit, NifDK, has proven difficult. Here, we find that when relocated plant mitochondria, NifD is subject errant peptidase-based cleavage insoluble. Guided by sequence variation amongst bacteria...
Plant storage compounds such as starch and lipids are important for human animal nutrition well industry. There is interest in diverting some of the carbon stored starch-rich organs (leaves, tubers, cereal grains) into order to improve energy density or nutritional properties crops providing new sources feedstocks food manufacturing. Previously, we generated transgenic potato plants that accumulate up 3.3% triacylglycerol (TAG) by dry weight which also led changes content, granule morphology...
The safflower (Carthamus tinctorius L.) is considered a strongly domesticated species with long history of cultivation. hybridization its wild relatives has played an important role in the evolution cultivars and particular interest regards to their production high quality edible oils. Original varieties were all rich linoleic acid, while oleic acid have risen prominence recent decades. trait controlled by partially recessive allele ol at single locus OL. was found be defective microsomal...
Cancer and stem cells share the ability to silence tumor suppressors. We focused on Lefty, which encodes one of most abundant suppressors in embryonic (ES) is not expressed somatic cancer cells. found that transforming growth factor β (TGF-β) induced demethylation Lefty B cytosine-phosphate-guanine (CpG) island increased expression (10-200 times) human pancreatic liver (PLC/PRF/5 HLF). Expression Cripto, another important Nodal-Lefty signaling, was after adding TGF-β. generated reprogrammed...
While industrial nitrogen fertilizer is intrinsic to modern agriculture, it expensive and environmentally harmful. One approach reduce usage engineer the bacterial nitrogenase enzyme complex within plant mitochondria, a location that may support function. Our current strategy involves fusing mitochondrial targeting peptide (MTP) (Nif) proteins, enabling their import matrix. However, process of modifies N-terminus each Nif protein impact assembly Here we present our workflow assessing...
The thermal profiles of 118 bacterial strains, representing six species the family Enterobacteriaceae, isolated from a variety native Australian mammals were determined under in vitro conditions. Each had unique profile and differed their minimum or maximum temperature for growth response to changing temperatures. taxonomic classification host which strains explained significant amount variation among species. Host effects detected at all levels: order, family, genus, locality (State...
Ancestral sequence reconstruction can be used to obtain highly thermostable catalysts of fatty acid hydroxylation.
An enantioselective total synthesis of (+)-anthecularin, an antiplasmodial and antitrypanosomal sesquiterpene lactone, has been achieved in 3.9% overall yield through 18 steps from a known dibromo alcohol. The key features the include intramolecular Claisen-type cyclization formyl-protected hydroxyl lactone to construct bicyclic intermediate with quaternary stereogenic center stereocontrolled 1,2-addition vinyllithium methoxyethyl-protected spirocyclic enone install tetrasubstituted...
Saturated mid-chain branched fatty acids are widely used in the petrochemical industry for their high oxidative stability and low melting temperature. Dihydrosterculic acid (DHSA) is a cyclopropane (CPA) that can be converted to SMCBFA via hydrogenation, therefore oils rich DHSA potential feedstock SMCBFA. Recent attempts produce seed oil by recombinant expression of synthases (CPFASes) resulted decreased content poor germination or accumulation. Here we explored plant vegetative tissue...
A long-held goal of synthetic biology has been the transfer a bacterial nitrogen-fixation pathway into plants to reduce use chemical fertiliser on crops such as rice, wheat and maize. There are three classes nitrogenase, named after their metal requirements, containing either MoFe-, VFe- or FeFe-cofactor, that converts N2 gas ammonia. Relative Mo-nitrogenase Fe-nitrogenase is not efficient for catalysis but less complex genetic metallocluster features may be preferable engineering crops....