A5042397941- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Clostridium difficile and Clostridium perfringens research
- Drug Transport and Resistance Mechanisms
- Gut microbiota and health
- Escherichia coli research studies
- Neuropeptides and Animal Physiology
- Pain Mechanisms and Treatments
- Microbial infections and disease research
- Receptor Mechanisms and Signaling
- Bacterial biofilms and quorum sensing
- Cardiac Ischemia and Reperfusion
- Hypothalamic control of reproductive hormones
- Animal Nutrition and Physiology
- Viral gastroenteritis research and epidemiology
- Neurotransmitter Receptor Influence on Behavior
- Insect and Pesticide Research
- Nitric Oxide and Endothelin Effects
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
- Animal Behavior and Welfare Studies
- Thyroid Disorders and Treatments
- Bacterial Identification and Susceptibility Testing
- Hormonal Regulation and Hypertension
CNR de la Résistance aux Antibiotiques
2019-2024
Innovations thérapeutiques et résistances
2019-2024
Université de Toulouse
2013-2023
École Nationale Vétérinaire de Toulouse
2008-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2023
Toxalim Research Centre in Food Toxicology
2011-2019
Université Toulouse III - Paul Sabatier
2013-2017
Institut National Polytechnique de Toulouse
2013-2017
École Normale Supérieure - PSL
1998
Centre National de la Recherche Scientifique
1995-1997
The aim of this study was to assess the impact three ampicillin dosage regimens on resistance among Enterobacteriaceae recovered from swine feces by use phenotypic and genotypic approaches. Phenotypically, determined percentage resistant MICs Escherichia coli isolates. pool genes also monitored quantification bla(TEM) genes, which code for most frequently produced beta-lactamases in gram-negative bacteria, using a newly developed real-time PCR assay. Ampicillin administered intramuscularly...
Quantitative data on fecal shedding of antimicrobial-resistant bacteria are crucial to assess the risk transmission from dogs humans. Our first objective was investigate prevalence quinolone/fluoroquinolone-resistant and beta-lactam-resistant Enterobacteriaceae in France Spain. Due particular concern about possible extended-spectrum cephalosporin (ESC)-resistant isolates their owners, we characterized ESBL/pAmpC producers collected dogs. Rectal swabs 188 dogs, without signs diarrhea that had...
ABSTRACT We demonstrate using an in vitro pharmacodynamic model that the likelihood of selection Escherichia coli mutants resistant to a fluoroquinolone was increased when initial size bacterial population, exposed concentrations within mutant window, increased.
The aim of this study was to examine the dynamics development resistance in fecal Escherichia coli populations during treatment with ampicillin for 7 days pigs. Before treatment, only 6% isolates were resistant, whereas more than 90% resistant after 4 and treatment. Ampicillin-resistant E. mainly multiresistant, 53% from treated pigs had one phenotype that included six antibiotics (ampicillin, chloramphenicol, sulfonamides, tetracycline, trimethoprim, streptomycin) at day 7. Determination...
Sewer biofilms are likely to constitute hotspots for selecting and accumulating antibiotic-resistant bacteria (ARB) antibiotic resistance genes (ARGs). This study aimed optimize culture conditions obtain in vitro biofilms, mimicking the biofilm collected sewers, impact of fluoroquinolones (FQs) on sewer microbiota. Biofilms were grown coupons CDC Biofilm Reactors®, continuously fed with nutrients inoculum (1/100 diluted wastewater). Different tested: (i) initial inoculum: wastewater or...
The pharmacokinetics of marbofloxacin was studied in eight healthy female Beagle dogs before and after moderate renal impairment induced experimentally. A single intravenous (i.v.) administration repeated for 8 days (2 mg/kg, once-a-day) were studied. Renal by a right kidney nephrectomy electrocoagulation the left kidney. An increase (P < 0.001) plasma concentrations urea (from 3.8+/-0.7 to 9.8+/-2.1 mmol/L) creatinine 78.8+/-3.4 145.8+/-22.3 micromol/L), significant decrease (2.9+/-0.3 vs...
The anatomical localization of neuropeptide FF receptors was examined by in vitro autoradiography techniques rat brain sections using [125I][D.Tyr1, (NMe)Phe3]NPFF. specific binding (NMe)Phe3]NPFF reached 90% the total at 0.05 nM spinal cord sections. Up to 40% still detectable following fixation with glutaraldehyde. Afterwards, distribution NPFF studied light microscopy and their densities microdensitometry an image analysis system. In microscope, labelling appeared more or less uniformly...
The aim of this study was to assess cyclooxygenase (COX)-1 and COX-2 expression in skeletal muscle after an ischemia-reperfusion (I/R). Male Sprague-Dawley rats were subjected unilateral hindlimb ischemia for 2 h then euthanized 0, 1, 2, 4, 6, 10, 24, 72 reperfusion. COX protein mRNA assessed control injured gastrocnemius muscle. Muscle damage indirectly determined by plasma creatine kinase activity edema weighing wet Creatine increased as early 1 reperfusion returned levels Edema observed...
The beta-lactam antibiotic ampicillin has a relatively poor oral bioavailability in animals and man (30-40%), its widespread agricultural use livestock may be contributing to the emergence of resistance environment. aim this study was define absorption mechanism by which crosses small intestinal epithelium. improved rat everted gut sac system used, with an emphasis on role PepT1 transporter. kinetics, effects pH competitive substrates failed provide any substantive evidence that transporter...
In the context of requested decrease antimicrobial use in veterinary medicine, our objective was to assess impact two doses marbofloxacin administered on young bulls (YBs) and veal calves (VCs) treated for bovine respiratory disease, total population Enterobacteriaceae gut flora emergence resistant Enterobacteriaceae. independent experiments, 48 YBs from 6 commercial farms 33 VCs previously colostrum deprived exposed cefquinome were randomly assigned one three groups LOW, HIGH, Control. LOW...
Wastewater treatment plants (WWTP) receiving effluents from food-producing animals and humans may contribute to the spread of extended-spectrum β-lactamases (ESBL)-carrying plasmids. This study was designed investigate cephalosporin resistant Escherichia coli strains, CTX-M distributions genetic lineage blaCTX-M -carrying plasmids urban slaughterhouse wastewaters. The level cephalosporin-resistant E. in wastewater entering WWTP negligible compared with that wastewater. blaCTX-M-1 gene...
Reducing antibiotic use is a necessary step toward less resistance in livestock, but many genes can persist for years, even an antibiotic-free environment. In this study, we investigated the potential of three fecal complex microbial communities from antibiotic-naive does to drive microbiota kits antibiotic-exposed dams and outcompete bacteria-carrying antibiotic-resistant genes. The were either orally delivered or simply added as fresh pellets four five nests that kept clean maternal feces....
Antibiotic resistance of microbes thriving in the animal gut is a growing concern for public health as it may serve hidden reservoir antibiotic genes (ARGs). We compared 16 control piglets to 24 fed 3 weeks with S1 or S2 fecal suspensions from two sows that were not exposed antibiotics at least 6 months: first suspension decreased erythromycin gene ermB and aminoglycoside phosphotransferase conferring kanamycine (aphA3), while second tetracycline tetL, an unexpected increase ARGs. Using 16S...
The aim of this study was to determine the percentage healthy veal calves carrying mcr-positive E. coli strains at time slaughter in France. Fecal samples were selectively screened for isolates using media supplemented with colistin. Screening mcr genes also carried out resistant critically important antimicrobials used human medicine recovered from same fecal samples. Overall, 28 (16.5%) 170 tested coli. As some several non-redundant strains, 41 recovered. Thirty-one and seven positive...
The administration of antibiotics can expose the digestive microbiota humans and animals to sub-inhibitory concentrations, potentially favouring selection resistant bacteria. minimal selective concentration (MSC) is a key indicator understand this process. MSC defined as lowest an antibiotic that promotes growth strain over susceptible isogenic strain. It represents lower limit sub-minimal inhibitory (MIC) window, where mutants be selected. Previous studies focused on determining under...
The increase of multidrug-resistant (MDR) bacteria has renewed interest in old antibiotics, such as minocycline, that can be active against various MDR Gram-negative pathogens. elimination minocycline by both kidneys and liver makes it suitable for impaired renal function patients. However, the drawback is possible a high amount drug intestines, with potential impact on digestive microbiota during treatment. This study aimed to predict activity Enterobacterales gut after parenteral...