A5020933906- Neonatal Respiratory Health Research
- Cystic Fibrosis Research Advances
- Parathyroid Disorders and Treatments
- Fibroblast Growth Factor Research
- Asthma and respiratory diseases
- Neonatal Health and Biochemistry
- Neuroscience of respiration and sleep
- Heme Oxygenase-1 and Carbon Monoxide
- Kruppel-like factors research
- Magnesium in Health and Disease
- Tracheal and airway disorders
- Nematode management and characterization studies
- Metabolism, Diabetes, and Cancer
- Biomedical Research and Pathophysiology
- Childhood Cancer Survivors' Quality of Life
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Vitamin D Research Studies
- Chemical Reactions and Isotopes
- Infant Health and Development
- Wnt/β-catenin signaling in development and cancer
- Thyroid and Parathyroid Surgery
Mahidol University
2018-2024
University of Bristol
2020-2024
Humboldt-Universität zu Berlin
2023
German Center for Lung Research
2023
Freie Universität Berlin
2023
Charité - Universitätsmedizin Berlin
2023
Abstract Some residues in the cystic fibrosis transmembrane conductance regulator (CFTR) channel are site of more than one CFTR variant that cause fibrosis. Here, we investigated function S1159F and S1159P, two variants associated with different clinical phenotypes, which affect same pore‐lining residue segment 12 both strongly potentiated by ivacaftor when expressed CFBE41o − bronchial epithelial cells. To study single‐channel behaviour CFTR, applied patch‐clamp technique to Chinese hamster...
Dysfunction of the epithelial anion channel cystic fibrosis transmembrane conductance regulator (CFTR) causes a wide spectrum disease, including (CF) and CFTR-related diseases (CFTR-RDs). Here, we investigate genotype-phenotype-CFTR function relationships using human nasal (hNE) cells from small cohort non-CF subjects individuals with CF CFTR-RDs genotypes associated either residual or minimal CFTR electrophysiological techniques. Collected hNE were studied directly whole-cell patch-clamp...
Abstract Background Pulmonary ionocytes have been identified in the airway epithelium as a small population of ion transporting cells expressing high levels CFTR (cystic fibrosis transmembrane conductance regulator), gene mutated cystic fibrosis. By providing an infinite source epithelial (AECs), use human induced pluripotent stem (hiPSCs) could overcome some challenges studying ionocytes. However, production AEC epithelia containing from hiPSCs has proven difficult. Here, we present...
Vasoactive intestinal peptide (VIP) as a neurocrine factor released by enteric neurons has been postulated to participate in the regulation of transcellular active calcium transport across epithelium, but preceding evidence is scant and inconclusive. Herein, transepithelial flux epithelial electrical parameters were determined Ussing chamber technique with radioactive tracer epithelium-like Caco-2 monolayer grown on Snapwell. After 3-day culture, cells expressed mRNA transporters, i.e.,...
The gasotransmitter carbon monoxide (CO) regulates fluid and electrolyte movements across epithelial tissues. However, its action on anion channels is incompletely understood. Here, we investigate the direct of CO cystic fibrosis transmembrane conductance regulator (CFTR) by applying CO-releasing molecules (CO-RMs) to intracellular side excised inside-out membrane patches from cells heterologously expressing wild-type human CFTR. Addition increasing concentrations...
Fibroblast growth factor (FGF)-21 is a salient liver-derived endocrine regulator for metabolism of glucose and triglyceride as well bone remodeling. Previously, certain peptides in the FGF family have been shown to modulate calcium absorption across intestinal epithelia. Since FGF21 receptor, i.e., receptor-1, abundantly expressed enterocytes, there was possibility that might exert direct actions on intestine. Herein, large-scale production recombinant at multi-gram level developed order...