A5102904260- Fullerene Chemistry and Applications
- Effects of Radiation Exposure
- Graphene and Nanomaterials Applications
- Carbon Nanotubes in Composites
- DNA Repair Mechanisms
- Radiation Therapy and Dosimetry
- Genomics, phytochemicals, and oxidative stress
- Tryptophan and brain disorders
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- RNA and protein synthesis mechanisms
- Mesenchymal stem cell research
- interferon and immune responses
- Geophysics and Sensor Technology
- MicroRNA in disease regulation
- Cystic Fibrosis Research Advances
- Mitochondrial Function and Pathology
- Anesthesia and Neurotoxicity Research
- Carcinogens and Genotoxicity Assessment
- Prenatal Screening and Diagnostics
- Stress Responses and Cortisol
- RNA Research and Splicing
- Pulsars and Gravitational Waves Research
Research Centre for Medical Genetics
2016-2025
Research Institute of Medical Genetics of Russian Academy of Medical Sciences
2007-2024
Federal Medical-Biological Agency
2022
V.A. Negovsky Scientific Research Institute of General Reanimatology
2016-2021
Sechenov University
2019-2021
Mental Health Research Center of Russian Academy of Medical Sciences
2018
Russian Academy of Sciences
2003-2015
Academy of Medical Sciences
2010-2012
Astro Space Center
2001-2006
Lomonosov Moscow State University
1996-2002
Introduction: The multi-copied genes coding for the human 18, 5.8, and 28S ribosomal RNA (rRNA) are located in five pairs of acrocentric chromosomes forming so-called rDNA. Human genome contains unmethylated, slightly methylated, hypermethylated copies major research question: What is rDNA copy number (rDNA CN) content as a function age? Materials Methods: We determined CN blood leukocyte genomes 651 subjects aged 17 to 91 years. were divided into two subgroups: "elderly" group (E-group, N =...
Introduction: Human blood normally contains circulating cell-free DNA (cirDNA). Cell-free (cfDNA) present in cell culture medium is termed extracellular (ecDNA). Its concentration, GC content and oxidation level depend on physiological state of the organism. cirDNA could probably be one aggressive factors encountered by therapeutic stem cells. The authors hypothesize that oxidized influence their survival rate. They aimed to uncover effects ecDNAs, including ecDNA cultivated primary tumor...
Oxidative stress is a major issue in wide number of pathologies (neurodegenerative, cardiovascular, immune diseases, and cancer). Because this, the search for new antioxidants an important issue. One potential that has been enthusiastically discussed past twenty years fullerene its derivatives. Although aqueous solutions derivatives have shown to be antioxidants, their properties this regard within cells are controversially discussed. We studied two different water-soluble C60 C70 on human...
The unique redox properties of nanoscale cerium dioxide determine its diverse application in biology and medicine as a regulator oxidative metabolism. Lipid modifiers the nanoparticle surface change their biochemical bioavailability. Complexes with lipids can be formed upon contact nanoparticles membrane. effects lipid coating on nanoceria have not been studied yet. Here, we assessed effect bare cardiolipin-coated CeO2 expression metabolism genes human embryonic lung fibroblasts. Cell...
We have hypothesized that the adaptive response to low doses of ionizing radiation (IR) is mediated by oxidized cell-free DNA (cfDNA) fragments. Here, we summarize our experimental evidence for this model. Studies involving measurements ROS, expression NOX (superoxide radical production), induction apoptosis and double-strand breaks, antiapoptotic gene cell cycle inhibition confirm hypothesis. demonstrated treatment mesenchymal stem cells (MSCs) with IR (10 cGy) leads death part population...
Introduction. Human satellite DNA organized in long arrays peri/centromeric heterochromatin. There is a little information about CNVs with aging and replicative cell senescence (RS). Materials Methods. Biotinylated pUC1.77 probe was used for the III (f-SatIII) quantitation leukocyte by non-radioactive quantitative hybridization 557 subjects between 2 91 years old. The effect of RS genotoxic stress (GS, 4µM or 6 µM K2CrO4) on f-SatIII CNV studied cultured skin fibroblast (HSF) lines five...
Introduction Increased systemic oxidative stress is common in schizophrenia (SZ) patients. NADPH-oxidase 4 (NOX4) the cell oxidoreductase, catalyzing hydrogen peroxide formation. Presumably, NOX4 main factor a number of diseases such as cardiovascular and cancer. We hypothesized that may be involved development caused by disease schizophrenic patients’ peripheral blood lymphocytes (PBL). Materials methods The SZ group included 100 patients (68 men 32 women aged 28 ± 11 years). control 60...
The level of apoptosis is increased during pregnancy. Dying cells emit DNA that remains in blood circulation and known as cell-free (cfDNA). concentration cfDNA can reflect the cell death. present article result studying DNase I activity plasma 40 non-pregnant women (control), healthy pregnant (over 37 weeks) with a diagnosis intrauterine growth restriction (IUGR). In order to explain obtained results, program modeling change under influence different internal external factors was written....
Schizophrenia (SZ) increases the level of cell death, leading to an increase in concentration circulating cell-free DNA (cfDNA). Ribosomal (rDNA) contains many unmethylated CpG motifs that stimulate TLR9-MyD88-NF-κB signaling and synthesis proinflammatory cytokines. The number rDNA copies genomes SZ patients is increased; therefore, we expect plasma also increases. This may be one explanations cytokine often observed SZ. major research question what copy cfDNA (cf-rDNA CN) its putative role...
The present study focuses on the investigation of oxidized cell-free DNA (cfDNA) properties in several experimental models, including cultured cerebellum cells, peripheral blood lymphocytes (PBL), plasma, and hippocampus under an acute chronic unpredictable stress model rats. Firstly, our shows that Spectrum Green fluorescence-labeled cfDNA fragments were transferred into cytoplasm 80% culture cells; meanwhile, nonoxidized do not pass cells. Oxidized stimulates antioxidant mechanisms...
The aim of this study was (1) to examine the leukocyte mtDNA copy number (CN) in unmedicated (SZ (m-)) and medicated (m+)) male patients with paranoid schizophrenia (SZ) comparison healthy controls (HC) (2) compare CN content an oxidation marker 8-oxodG lymphocytes SZ (m-) patients.We evaluated 110 subjects 60 HC by method qPCR (ratio mtDNA/nDNA (gene B2M) detected). were divided into two subgroups. subgroups (m+) (N = 55) treated standard antipsychotic medications hospital. subgroup not...
Cell‐free DNA (cfDNA) is a circulating of nuclear and mitochondrial origin mainly derived from dying cells. Recent studies have shown that cfDNA stress signaling DAMP (damage‐associated molecular pattern) molecule. We report here the expression profiles cfDNA‐induced factors NRF2 NF‐ κ B are distinct depending on target cell’s type GC‐content oxidation rate cfDNA. Stem cells (MSC) higher without inflammation in response to In contrast, inflammatory launched by was dominant differentiated...
We demonstrate that a series of vat dyes, which are commonly used for textile coloration, represent promising organic semiconductors with low toxicity and high biocompatibility, the key requirements wearable on-skin electronics.
Background . Cell free DNA (cfDNA) circulates throughout the bloodstream of both healthy people and patients with various diseases. CfDNA is substantially enriched in its GC-content as compared human genomic DNA. Principal Findings Exposure haMSCs to GC-DNA induces short-term oxidative stress (determined H2DCFH-DA) results single- double-strand breaks (comet assay γ H2AX, foci). As a result cells significantly increases expression repair genes ( BRCA1 (RT-PCR), PCNA (FACS)) antiapoptotic...