A5039067070- Cardiovascular and exercise physiology
- Cardiomyopathy and Myosin Studies
- Heart Rate Variability and Autonomic Control
- Hemodynamic Monitoring and Therapy
- Amyotrophic Lateral Sclerosis Research
- Cardiovascular Function and Risk Factors
- Non-Invasive Vital Sign Monitoring
- Neuroscience and Neuropharmacology Research
- Cardiac Imaging and Diagnostics
- Ion channel regulation and function
- Neurogenetic and Muscular Disorders Research
- Cardiac Valve Diseases and Treatments
- Neuroscience and Neural Engineering
- Congenital Heart Disease Studies
- Cardiovascular Effects of Exercise
- Blood Pressure and Hypertension Studies
- Mechanical Circulatory Support Devices
- Infant Development and Preterm Care
- Cardiac Health and Mental Health
- Aortic Disease and Treatment Approaches
- Retinal Development and Disorders
- Mobile Health and mHealth Applications
- Free Radicals and Antioxidants
- Cholinesterase and Neurodegenerative Diseases
- Transplantation: Methods and Outcomes
Clinical Research Institute
2024
Newcastle upon Tyne Hospitals NHS Foundation Trust
2019-2024
Newcastle University
2000-2024
University of Newcastle Australia
2024
Newcastle upon Tyne Hospital
2016
University of Sunderland
2015
Royal Victoria Infirmary
1999-2000
University of Sheffield
1995-1996
University of California, Los Angeles
1970-1972
Abstract: Modification of the growth conditions NSC‐34 mouse neuroblastoma × motor neurone cells by serum depletion promotes expression functional glutamate receptors as mature into a form that bears phenotypic characterisation neurones. Immunocytochemical studies demonstrated presence receptor proteins NMDAR1, NMDAR2A/B, GluR1, GluR2, GluR2/3, GluR4, GluR6/7, and KA2. Toxicity assays using cell counting techniques mild but significant death (∼30%, p < 0.01) following 24‐h exposure to 1 m...
Disturbance of glutamate neurotransmission may contribute to motor neuron injury in amyotrophic lateral sclerosis. There is evidence that human neurons express a specific profile receptors, with low or absent expression mRNA for the GluR2 AMPA receptor subunit, which has crucial role controlling calcium permeability. This study, using an immunocytochemical approach antibody, shows upper and lower have very low/absent protein, contrast many other neuronal groups. Thus, it likely high...
The exact molecular mechanisms by which mutations in Cu/Zn superoxide dismutase (SOD1) cause motor neuron injury remain incompletely understood, though a body of evidence suggests that the mutant protein exerts cell-specific toxic gain function. role nitric oxide (NO) SOD1-related has been particularly controversial. Theoretically, there are arguments to suggest NO may exert an important injury, but is relatively little direct experimental support for this hypothesis.The present study aimed...
Objective Exercise intolerance is a clinical hallmark of chronic conditions. The present study determined pathophysiological mechanisms exercise in cardiovascular, neuromuscular, and metabolic disorders. Methods In prospective cross-sectional observational 152 patients (heart failure reduced ejection fraction, n=32; stroke, n=34; mitochondrial disease, n=28; type two diabetes, healthy controls, n=30) performed cardiopulmonary testing with haemodynamic measurements. Peak O 2 consumption...
Objective Diminished cardiac high-energy phosphate metabolism (phosphocreatine-to-ATP (PCr:ATP) ratio) and power with age may play an important roles in development of dysfunction heart failure. The study defines the impact on PCr:ATP ratio their relationship. Methods Thirty-five healthy women (young≤50 years, n=20; old≥60 n=15) underwent MRI 31 P spectroscopy to assess performed maximal graded cardiopulmonary exercise testing simultaneous gas-exchange central haemodynamic measurements. Peak...
Summary Purpose This study assessed the agreement between cardiac output estimated by inert gas rebreathing and bioreactance methods at rest during exercise. Methods Haemodynamic measurements were in 20 healthy individuals (11 females, nine males; aged 32 ± 10 years) using methods. Gas exchange haemodynamic data measured simultaneously under different stages (i.e. 30, 60, 90, 120, 150 180 W) of progressive graded cardiopulmonary exercise stress testing a bicycle ergometer. Results At rest,...
Abstract At present there are two recognised guidelines for the echocardiographic assessment of left ventricular diastolic function provided by British Society Echocardiography and American Echocardiography/European Association Cardiovascular Imaging. However, no direct comparison these has been performed to establish whether they provide similar grading. One hundred eighty-nine consecutive patients in sinus rhythm who underwent transthoracic echocardiography a primary indication either...
Age and physical inactivity are important risk factors for cardiovascular mortality. Heart rate response to exercise (HRRE) heart recovery (HRR), measures of cardiac autonomic function, strong predictors The present study defined the effect age activity on HRRE HRR. Healthy women (N=72) grouped according (young, 20-30 years; middle, 40-50 older, 65-81 years) daily (low active <7500, high >12,500 steps/day) performed a maximal cardiopulmonary test. was as an increase in from rest 1, 3 5...
Abstract The pattern of arterial supply to the cat's lumbosacral spinal cord was examined in a dozen latex‐injected specimens with view determining what precautions might be taken procedure rhizotomy minimize embarrassment cord's circulation. single ventral artery found receive on an average 4.1 significant radicular contributions, which one or two arteries at fourth third lumbar levels left were invariably especially large size. Equal numbers, about six eight, contributions dorsolateral...
Abstract Non‐invasive technologies have become popular for the clinical evaluation of cardiac function. The present study evaluated hemodynamic response to cardiopulmonary exercise stress testing using bioreactance technology in patients with hypertrophic cardiomyopathy. included 29 HCM (age 55 ± 15 years; 28% female) and 12 age (55 14 years), gender matched (25% healthy controls. All participants underwent maximal graded simultaneous non‐invasive gas exchange. At rest, demonstrated...
The present study evaluated reproducibility of the inert gas rebreathing method to estimate cardiac output at rest and during cardiopulmonary exercise testing. Thirteen healthy subjects (10 males, 3 females, ages 23-32 years) performed maximal graded stress test using a cycle ergometer on 2 occasions (Test 1 Test 2). Participants cycled 30-watts/3-min increments until peak exercise. Hemodynamic variables were assessed different intensities (i. e., 60, 120, 150, 180 watts) an technique....
Direct real-time electrochemical measurements have offered new insight into the importance of free radical interplay in a number cellular models.Initially interrelationship between O 2 and NO generation astrocytic, neuronal mixed astrocytic/neuronal cell populations was examined.Results indicated novel function for astrocytic nitric oxide synthase (cNOS) regulating extracellular -release therefore controlling availability.Further, pathological consequences -production on rat astrocytes...
Background A limited number of studies have investigated the effects lifestyle interventions in hypertrophic cardiomyopathy (HCM). This study evaluated effect a novel intervention incorporating physical activity (PA) and dietary nitrate supplementation on heart rate variability (HRV) haemodynamic measures HCM.
<h3>Introduction</h3> Engagement in regular physical activity reduces symptom burden and improves quality of life individuals with hypertrophic cardiomyopathy (HCM) those living heart failure (HF). This study evaluated the activity, inactivity sleep HCM versus HF. <h3>Methods</h3> Twenty-one (52±15 years old, body mass index (BMI) 29 (26–31) kg/m2) eighteen stable chronic HF reduced ejection fraction (68±7 BMI: 27 (25–31) kg/m2, left ventricular fraction: 35 (30–35) %) completed 7-day...
Background: Hypertrophic cardiomyopathy (HCM) is a global heart disease with great variability in severity, which can lead to significant impairment of exercise capacity. In healthy populations, oxygen consumption related cardiac output and arteriovenous difference. We sought determine the relationship between hemodynamics (cardiac stroke volume) HCM compared controls during maximal stress testing. Aims: To evaluate associations trajectories hemodynamic function controls. Methods: Twenty...
Heart rate variability (HRV) is a measure of cardiac autonomic function. This study: (1) evaluated the differences in HRV and haemodynamic function between individuals with hypertrophic cardiomyopathy (HCM) healthy controls, (2) determined relationship variables HCM.Twenty-eight HCM (n = 7, females; age 54 ± 15 years; body mass index: 29 5 kg/m2 ) 28 matched 7 16 completed 5-min measurements under resting (supine) conditions using bioimpedance technology. Frequency domain measures (absolute...
Aortic dissection is often regarded as a catastrophic aortic syndrome with high rates of mortality. The sensitivity and specificity transthoracic echocardiography when diagnosing acute type A has been reported 97% 100%, respectively, in patients optimal imaging quality compared to computed tomography. In this article, we discuss the benefit patient extending from ascending aorta iliac arteries.
The cardiac action potential reflects change in the electrical activity of individual heart muscle cells. This occurs as a consequence sodium, potassium and calcium ions moving across cell membrane through protein-based structures known ion channels. Alteration to channel structure function affects potential. Consequently, drugs that block channels or genetic mutations result abnormal ion-channel formation heart-muscle article explains appearance ventricular cells pacemaker sinoatrial node....
<h3>Objective</h3> To enable evidence-based public health policy and guidelines for 24-hour behaviours in infants toddlers, by demonstrating the feasibility acceptability of using valid, objective, longitudinal measures everyday behaviour physiology toddlers. <h3>Methods</h3> We are currently recruiting, through non-NHS community partners, children age 4–36 months, measuring their 24hr physical activity, sleep stress as well related within lives five days, a combination wearable sensors (a...