A5078781679- Cellular Mechanics and Interactions
- Micro and Nano Robotics
- Microfluidic and Bio-sensing Technologies
- Cell Adhesion Molecules Research
- Caveolin-1 and cellular processes
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Sphingolipid Metabolism and Signaling
- Environmental DNA in Biodiversity Studies
- Optical Coherence Tomography Applications
- Near-Field Optical Microscopy
- Vibrio bacteria research studies
- Platelet Disorders and Treatments
- Force Microscopy Techniques and Applications
- Genomics and Phylogenetic Studies
- Identification and Quantification in Food
- Cerebrospinal fluid and hydrocephalus
- 3D Printing in Biomedical Research
- Advanced Fluorescence Microscopy Techniques
- Digital Holography and Microscopy
Living Systems (United States)
2020-2023
Aix-Marseille Université
2018-2023
Inserm
2019-2023
Laboratoire Adhésion et inflammation
2018-2023
Centre National de la Recherche Scientifique
2019-2020
Centre d’Immunologie de Marseille-Luminy
2020
<ns3:p>Marine sponges have emerged as effective natural samplers of environmental DNA (eDNA), offering a promising alternative for biodiversity monitoring. By filtering large volumes seawater, accumulate eDNA from surrounding communities, potentially enhancing species detection in marine environments where conventional water sampling is limited. In this study, we evaluated the recovery efficiency seven Mediterranean sponge to identify optimal candidates biomonitoring. <ns3:italic>Axinella...
Permeable agarose barriers allow flow-free gradient generation, applicable to adherent and non-adherent (swimming) cells, as well co-culture experiments.
Naive T lymphocytes traffic through the organism in search for antigen, alternating between blood and secondary lymphoid organs. Lymphocyte homing to lymph nodes relies on CCL21 chemokine sensing by CCR7 receptors, while exit into efferent lymphatics sphingolipid S1P S1PR1 receptors. While both molecules are claimed chemotactic, a quantitative analysis of naive lymphocyte migration along defined gradients is missing. Here, we used reductionist approach study real-time single-cell response...
Background Information Leukocytes migrate in an amoeboid fashion while patrolling our organism the search for infection or tissue damage. Their capacity to has been proven integrin independent, however, non‐specific adhesion confinement remain a requisite current models of cell migration. This idea challenged twice within last decade with human neutrophils and effector T lymphocytes, which were shown free suspension, phenomenon termed swimming. While relevance leukocyte swimming vivo remains...
Macroscopic properties of physical and biological processes like friction, wetting, adhesion or cell migration are controlled by interfacial at the nanoscopic scale. In an attempt to bridge simultaneously investigations different scales, we demonstrate here how optical microscopy in Wet-Surface Ellipsometric Enhanced Contrast (Wet-SEEC) mode offers imaging measurement thin films solid/liquid interfaces range 1–500 nm with lateral resolution. A live, label-free noninvasive methodology...
Immune cells have the ubiquitous capability to migrate disregarding adhesion properties of environment, which requires a versatile adaptation their adhesiveness mediated by integrins, family specialized proteins. Each subtype integrins has several ligands and affinity states controlled internal external stimuli. However, probing cell on live without perturbing motility is highly challenging, especially in vivo . Here, we developed novel vitro method using micron-size beads pulled flow...
ABSTRACT Mammalian cells developed two main migration modes. The slow mesenchymatous mode, like fibroblasts crawling, relies on maturation of adhesion complexes and actin fiber traction, while the fast amoeboid observed exclusively for leukocytes cancer cells, is characterized by weak adhesion, highly dynamic cell shapes, ubiquitous motility 2D in 3D solid matrix. In both cases, interactions with substrate or friction are widely accepted as a prerequisite mammalian motility, which precludes...
Biomedical imaging lacks label-free microscopy techniques able to reconstruct the contour of biological cells in solution, 3D and with high resolution, as required for fast diagnosis numerous diseases. Inspired by computational optical coherence tomography techniques, we present a tomographic diffractive microscope reflection geometry used synthetic confocal microscope, compatible this goal validated reconstruction human effector T lymphocyte.
Abstract The recruitment of leukocytes from blood vessels to inflamed zones is guided by biochemical and mechanical stimuli, with mechanisms only partially deciphered. We studied here the guidance flow primary human effector T lymphocytes crawling on substrates coated ligands integrins LFA-1 (α L β 2 ) VLA-4 4 1 ), showed that cells segregated in two populations opposite orientation for combined adhesion. Sharp decisions were shown rely a bistable mechanism between LFA-1-mediated upstream...
Summary Naïve T lymphocytes traffic through the organism in their search for antigen, alternating between blood and secondary lymphoid organs. Lymphocyte homing to lymph nodes relies on chemokine CCL21, while exit into efferent lymphatics sphingolipid S1P. Surprisingly, both molecules are claimed chemotactic, a quantitative analysis of naïve lymphocyte migration along defined gradients is missing. Here, we used reductionist vitro approach study real-time, single-cell response CCL21 S1P-rich...