Nanoparticles (NPs) are becoming increasingly widespread in the environment. Free cadmium ions released from commonly used NPs under ultraviolet-B (UV-B) radiation potentially toxic to living organisms. With increasing levels of UV-B at Earth's surface due depletion ozone layer, potential additive effect and on plants is concern. In this study, we investigated synergistic CdTe quantum dots (CdTe-QDs), a common form NP, wheat seedlings. Graded doses CdTe-QDs were tested, either alone or...

The level of ultraviolet-B (UV-B) radiation on the Earth's surface has increased due to depletion ozone layer. Here, we explored effects continuous wave He-Ne laser irradiation (632 nm, 5 mW mm–2, 2 min d–1) physiological indexes wheat seedlings exposed enhanced UV-B (10 KJ m–2 at early growth stages. Wheat were irradiated with UV-B, treatment or a combination two. Enhanced had deleterious photosynthesis parameters including photosystem II (chlorophyll content, Hill reaction, chlorophyll...

Increasing evidence has accrued which indicates that mesenchymal stem cells (MSCs) have a potential clinical value in the treatment of certain diseases. Globally, nonalcoholic steatohepatitis (NASH) is widespread disorder. In present study, MSCs were isolated successfully from compact bone and mouse model NASH was established as achieved with use methionine-choline deficient (MCD) diet. Compact bone-derived transplantation reduced MCD diet-induced weight loss, hepatic lipid peroxidation,...

Although the multipotency of mesenchymal stem cells (MSCs) makes them an attractive choice for clinical applications, immune modulation is important factor affecting MSC transplantation. At present, effect treatment with MSCs on non‑alcoholic fatty liver disease (NAFLD) has received little attention. In present study, a compact bone‑derived method was used to isolate mouse (mMSCs) and high‑fat diet establish model NAFLD. Immunophenotypic features mMSCs were analyzed using flow cytometry....

Summary The timely removal of end‐of‐purpose flowering organs is as essential for reproduction and plant survival flowering. Despite much progress in understanding the molecular mechanisms floral organ abscission, little known about how various environmental factors are integrated into developmental programmes that determine timing abscission. Here, we investigated whether reactive oxygen species (ROS), mediators stress‐related signalling pathways, involved determining abscission and, if so,...

Bacterial wilt (BW) is a serious disease that affects potato (Solanum tuberosum L.) production. Although resistance to this has been reported, the underlying mechanism unknown. In study, we identified NAC family transcription factor (StNACb4) from and characterised its structure, function, expression, localisation at tissue role in BW resistance. To end, transgenic Nicotiana benthamiana Domin lines were generated which expression of NACb4 was constitutively upregulated or suppressed using...

Enhanced ultraviolet-B (UV-B) radiation is caused by the thinning ozone and affects photosynthesis crop yield. Recently, UV-B has been considered as an environmental signal that regulates plant growth. Elucidating downstream effectors in UV-B-triggered pathways of particular interest. Previous studies have shown actin filaments (AFs) play many roles during cell physiological processes. However, underlying response AFs to remains unclear. In this study, wheat protoplasts were isolated from...

The level of ultraviolet-B (UV-B) radiation on the Earth’s surface has increased due to depletion ozone layer. Here, we explored effects continuous wave He-Ne laser irradiation (632 nm, 5 mW·mm-2, 2 min·d-1) proliferating-cell nuclear antigen (PCNA) damage repair function wheat seedlings exposed enhanced UV-B (10.08 kJ&middotm-2·d-1) at early growth stages. Wheat were irradiated with UV-B, treatment or a combination two. We transcripts PCNA in each group using RT-PCR. In addition, total...

Abstract Background UV-B signaling in plants is mediated by UVR8, which interacts with transcriptional factors to induce root morphogenesis. However, research on the downstream molecules of UVR8 roots still scarce. As a wide range functional cytoskeletons, how actin filaments respond UV-B-induced morphogenesis has not been reported. The aim this study was investigate effect under and hydrogen peroxide exposure Arabidopsis. Results A Lifeact-Venus fusion protein used stain Arabidopsis ....

Possible mechanisms of N-CD toxicity to <italic>Arabidopsis</italic>.

Cytoskeleton exists in all eukaryotes and is involved many significant cytobiological processes, especially the movements developmental changes of plant cells. The cytoskeleton consists microtubule (MT), microfilament (MF), intermediate filament (IF). MT MF are vital components cytoskeleton. Crosslinking factor acts as a bridge between MT. They play an important role cellular life process have always been hot topic key point cytobiology, IF difficult this field. In paper, latest research on...

Enhanced ultraviolet-B (UV-B) radiation causes chromosomal aberrations and abnormal microtubule arrays in wheat (Triticum aestivum) root cells.Oryzalin induces chromosome doubling through depolymerization of microtubules results diploidization.We explored the effect oryzalin on roots exposed to enhanced UV-B radiation.Treatment with 500 µmol•L -1 resulted two equal nuclei.However, exposure combination unequal nuclei.The frequency nuclei caused by treatment was higher than that alone.During...

The Ultraviolet Resistance Locus 8 (UVR8) in plants recognizes ultraviolet-B (UV-B) light and plays a crucial role regulating plant growth through series of signal transduction events. However, the UVR8 monocotyledon crops has not yet been systematically analysed. We identified BdUVR8 (BRADI_3g45740) from genome Brachypodium distachyon, relative wheat, by analysing phylogenetic tree, gene expression pattern, detecting accumulation UV-B response metabolites, checking for phenotype recovery....

Previously isolated human placental cDNA clones represent a fusion of specific RNA sequences encoded by two genes: lysosomal chitobiase (CTB) and G-protein γ&lt;sub&gt;5&lt;/sub&gt; subunit (GNG5, Fisher Aronson, 1992a, 1992b). Both genes have now been mapped to 1p PCR analysis somatic cell hybrids further refined 1p22 fluorescence in situ hybridization (FISH) using YAC clone that contains both the 75 genes.

Being sessile, plants are continuously exposed to DNA-damaging agents presenting in the environment such as ultraviolet (UV).Sunlight acts an energy source for photosynthetic plants; hence, avoidance of UV radiations (namely, UV-A, 315 -400 nm; UV-B, 280 -315 and UV-C, <280 nm) is unpreventable.DNA particular strongly absorbs UV-B; therefore, it most important target UV-B induced damages.This paper mainly used different doses enhanced radiation (B 1 group: 4.05 kJ•m -2 •d -1 , B 2 10.08 3...

This work investigated the use of a He?Ne laser in alleviating damaging effects ultraviolet-B (UV-B) radiation on wheat seedlings by influenced actin filaments. Triticum aestivum were irradiated with either enhanced UV-B (10.08?KJ?m?2?d?1) or combination light and laser. Plants also exposed to alone. In order compare effect laser, red (same power wavelength as laser) treatment combined added. Moreover, treated special drugs, including cytochalasin B (CB) jasplakinolide (JAS). We analyzed...

VIGS (Virus-induced gene silencing) can effectively silence target genes at the RNA level to investigate their functions. The virus vectors used for silencing are divided into three categories, DNA virus, and satellite which be in different species based on existing research. as markers system identification include PDS PCNA. applied some plants, particular cash crops fruit trees, study disease-resistance related growth development provide a basis improving crop characteristics.