A5101895965- Renal and related cancers
- Renal cell carcinoma treatment
- Mesenchymal stem cell research
- Gut microbiota and health
- RNA Research and Splicing
- Acupuncture Treatment Research Studies
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Genetic and Kidney Cyst Diseases
- Inflammatory Bowel Disease
- Organ Donation and Transplantation
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Myofascial pain diagnosis and treatment
- Dermatology and Skin Diseases
- Ion channel regulation and function
- Venomous Animal Envenomation and Studies
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Healthcare and Venom Research
- Genomics and Chromatin Dynamics
- Immune cells in cancer
University of Southern California
2020-2024
Broad Center
2023-2024
Hunan Normal University
2018-2023
Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine
2022-2023
Chengdu University of Traditional Chinese Medicine
2017-2020
Chinese University of Hong Kong
2016-2019
Chinese University of Hong Kong, Shenzhen
2019
Tianjin Medical University
2013-2017
Tianjin Infectious Diseases Hospital
2016
Ministry of Education of the People's Republic of China
2015
Orthotopic liver transplantation is the only effective treatment for failure but limited with shortage of available donor organs. Recent studies show promising results mesenchymal stem cells (MSCs)-based therapies. We systematically investigate therapeutic effects MSCs or MSC-conditioned medium (MSC-CM) in ameliorating fulminant hepatic (FHF) and chronic fibrosis mice. In addition, extensive flow cytometry analysis spleens from vehicle MSC- MSC-CM-treated mice was applied to reveal...
Abstract Current kidney organoids model development and diseases of the nephron but not contiguous epithelial network kidney’s collecting duct (CD) system. Here, we report generation an expandable, 3D branching ureteric bud (UB) organoid culture that can be derived from primary UB progenitors mouse human fetal kidneys, or generated de novo pluripotent stem cells. In chemically-defined conditions, generate CD organoids, with differentiated principal intercalated cells adopting spatial...
Abstract Adiponectin is an adipocyte-derived circulating protein with beneficial effects on injured livers. Adiponectin-deficient (adipo(−/−)) mice develop enhanced liver fibrosis, suggesting that adiponectin could be a therapeutic target for injury. In the present study, we investigated protective role of ADP355, adiponectin-based active short peptide, in thioacetamide (TAA)-induced acute injury and chronic fibrosis mice. ADP355 remarkably reduced TAA-induced necroinflammation fibrosis....
Previous studies have confirmed that acupuncture and moxibustion is an effective way for treating ulcerative colitis (UC). However, the exact mechanism unclear yet. In this study, DSS-induced UC mice were treated by electroacupuncture moxibustion, genome of intestinal flora was subsequently detected high-throughput sequencing in order to explore detailed terms flora. The results indicated alpha diversity indices beta improved treatments, especially treatment. These treatments inhibited...
Epigenomic changes and stem cell deterioration are two hallmarks of aging. Accumulating evidence suggest that senescence mesenchymal stromal cells (MSCs) perpetuates aging or age-related diseases. Here we report H3K9 demethylases, KDM3A KDM4C, regulate heterochromatin reorganization via transcriptionally activating condensin components NCAPD2 NCAPG2 during MSC senescence. Suppression KDM4C by either genetic biochemical approach leads to robust DNA damage response aggravates cellular...
Autologous mesenchymal stem cells (MSC) are widely used cell source for cell-based tissue repair and regeneration, but replicative senescence the associated loss of cellular activity during in vitro expansion limit their therapeutic potential. How to preserve or even enhance proliferation differentiation ability MSC from aged donors without genetic modification remains a challenge meet clinical need. isolated human fetal tissues (hFMSC) exhibit higher activities prolonged culture, which...
Aging is characterized by the accumulation of proteins that display amyloid-like behavior. However, molecular mechanisms which these arise remain unclear. Here, we demonstrate are produced in a variety human cell types, including stem cells, brain organoids and fully differentiated neurons mistakes occur messenger RNA molecules. Some generate mutant already known to cause disease, while others have not been observed before. Moreover, show increase when cells exposed DNA damage, major...
To determine the error rate of transcription in human cells, we analyzed transcriptome H1 embryonic stem cells with a circle-sequencing approach that allows for high-fidelity sequencing transcriptome. These experiments identified approximately 100,000 errors distributed over every major RNA species cells. Our results indicate different display rates, suggesting prioritize fidelity some RNAs others. Cross-referencing detected various genetic and epigenetic features genome revealed vivo...
Aim. To investigate the relationship between effects of electroacupuncture/moxibustion and balance Th17/Treg in treating ulcerative colitis (UC) to preliminary compare above two methods. Methods. DSS‐induced UC mice were treated by electroacupuncture moxibustion. Disease activity index (DAI) was scored; intestinal pathological structure ultrastructure observed. The levels IL‐2, IL‐6, IL‐10, IL‐17A, IL‐17F, TGF‐ β plasma measured ELISA. percentages Treg Th17 spleen lymphocytes analyzed flow...
Although the multipotency of mesenchymal stem cells (MSCs) makes them an attractive choice for clinical applications, immune modulation is important factor affecting MSC transplantation. At present, effect treatment with MSCs on non‑alcoholic fatty liver disease (NAFLD) has received little attention. In present study, a compact bone‑derived method was used to isolate mouse (mMSCs) and high‑fat diet establish model NAFLD. Immunophenotypic features mMSCs were analyzed using flow cytometry....
Background and objectives Diabetes mellitus is a serious metabolic disease that causes economic burden worldwide. Gut flora major component of diabetes research, the aim this study was to understand trends components research related gut in last 11 years. Methods We searched Web Science Core Collection database for articles on from 2011-2021 July 2, 2022. The literature data were analyzed country, institution, author, steward, journal, highly cited using Citespace.5.8.R3 Vosviewer1.6.17....
Psychological disorders are prevalent in patients with inflammatory bowel disease, but the underlying mechanisms remain unknown. The aim of this study was to whether electroacupuncture (EA) and moxibustion (MB) can improve anxiety behavior DSS-induced colitis mice investigate effect is related hypothalamic-pituitary-adrenocortical (HPA) axis. model established by drinking 2.5% dextran sodium sulfate (DSS). were treated EA or MB. Disease activity index (DAI) scored; intestinal morphological...
Spider venoms contain a vast array of bioactive peptides targeting ion channels. A large number have high potency and selectivity toward sodium Nav1.7 contributes to action potential generation propagation participates in pain signaling pathway. In this study, we describe the identification µ-TRTX-Ca2a (Ca2a), novel 35-residue peptide from venom Vietnam spider Cyriopagopus albostriatus (C. albostriatus) that potently inhibits (IC50 = 98.1 ± 3.3 nM) with against skeletal muscle isoform Nav1.4...
The role of B cells in the pathogenesis hepatitis virus (HBV) infection has not been explored depth. In present study, activation status from peripheral blood healthy controls (N = 20) and patients with acute (AHB, N 15) or chronic (CHB, 30) was evaluated by measuring expression levels B-cell markers CD69 CD86, using quantitative real-time PCR flow cytometry. Moreover, potential mechanism underlying during HBV further investigated analyzing profile FCRL1, an intrinsic molecule cells. An...
Various peptide toxins in animal venom inhibit voltage-gated sodium ion channel Nav1.7, including Nav-targeting spider toxin (NaSpTx) Family I. Toxins NaSpTx I share a similar structure, i.e., N-terminal, loops 1–4, and C-terminal. Here, we used Mu-theraphotoxin-Ca2a (Ca2a), isolated from <i>Cyriopagopus albostriatus</i>, as template to investigate the general properties of The interacted with cell membrane prior binding Nav1.7 via hydrophobic residues. Residues loop 1, 4, C-terminal...
Background and aim Ulcerative colitis is a chronic inflammatory bowel disease, intestinal flora plays an important role in ulcerative colitis. In this study, we conducted bibliometric analysis of publications the field research past 10 years to summarize current status analyze trends field. Methods On July 15, 2022, chose Web Science Core Collection database as study’s data source. CiteSpace.5.8.R3 VOSviewer 1.6.17 were used examine on that published between 2012 2021. We looked through...
Hemostasis is a crucial process that quickly forms clots at injury sites to prevent bleeding and infections. Dysfunctions in this can lead hemorrhagic disorders, such as hemophilia thrombocytopenia purpura. While hemostatic agents are used clinical treatments, there still limited knowledge about potentiators targeting coagulation factors. Recently, LCTx-F2, procoagulant spider-derived peptide, was discovered. This study employed various methods, including chromogenic substrate analysis...