A5029906837- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Essential Oils and Antimicrobial Activity
- Listeria monocytogenes in Food Safety
- Gastrointestinal motility and disorders
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Mycobacterium research and diagnosis
- Animal Nutrition and Physiology
- Moringa oleifera research and applications
- Liver Disease Diagnosis and Treatment
- Microscopic Colitis
- Pharmacological Effects of Natural Compounds
- Probiotics and Fermented Foods
- Food Safety and Hygiene
- IL-33, ST2, and ILC Pathways
- Vibrio bacteria research studies
- Cassava research and cyanide
- Herbal Medicine Research Studies
- Plant Pathogenic Bacteria Studies
- Mycotoxins in Agriculture and Food
- Nosocomial Infections in ICU
- Ginseng Biological Effects and Applications
- Urinary Tract Infections Management
- Infectious Diseases and Mycology
Auburn University
2023-2025
Iowa State University
2019-2024
University of Connecticut
2013-2017
Clostridium difficile is a nosocomial pathogen that causes serious toxin-mediated enteric disease in humans. Reducing C. toxin production could significantly minimize its pathogenicity and improve outcomes This study investigated the efficacy of two, food-grade, plant-derived compounds, namely trans-cinnamaldehyde (TC) carvacrol (CR) reducing cytotoxicity vitro. Three hypervirulent isolates were grown with or without sub-inhibitory concentrations TC CR, culture supernatant bacterial pellet...
Salmonella enterica serovar Enteritidis is a major foodborne pathogen in the United States, causing gastroenteritis humans, primarily through consumption of contaminated eggs. Chickens are reservoir host S. Enteritidis. In layer hens, colonizes intestine and migrates to various organs, including oviduct, leading egg contamination. This study investigated efficacy in-feed supplementation with trans-cinnamaldehyde (TC), generally recognized as safe (GRAS) plant compound obtained from cinnamon,...
Salmonella Enteritidis is a common foodborne pathogen transmitted to humans largely by consumption of contaminated eggs. The external surface eggs becomes with from various sources on farms, the main being hens' droppings and litter. Therefore, effective egg disinfection critical reduce pathogens potentially control egg-borne disease outbreaks. This study investigated efficacy GRAS (generally recognized as safe) status, plant-derived antimicrobials (PDA), namely trans-cinnamaldehyde (TC),...
Gastrointestinal illnesses and dysbiosis are among the most common comorbidities reported in patients with neurodevelopmental disorders. The manuscript reports that C. difficile infection (CDI), predisposed by antibiotic-induced gut dysbiosis, causes significant alterations dopamine metabolism major dopaminergic brain regions mice (P < 0.05). In addition, infected exhibited significantly reduced beta-hydroxylase (DBH) activity compared to controls 0.01). Moreover, a increased serum...
In this study, we investigated the therapeutic efficacy of plant compounds trans-cinnamaldehyde (TC) and eugenol (EG) on reducing Salmonella enterica serovar Enteritidis (SE) in commercial, market-age broiler chickens. Straight run, day-old commercial chicks (N = 84) were randomly grouped into 6 groups 14 birds each (n 14/group): a negative control (no SE, no TC or EG), EG 1% 0.75% TC), positive (SE, an challenge group TC). Before start experiment, flock was screened for any inherent 12...
Abstract This study investigated the efficacy of 3 GRAS‐status, plant‐derived antimicrobials (PDAs), trans ‐cinnamaldehyde (TC), carvacrol (CR), and β‐resorcylic acid (BR) applied as an antimicrobial wash for killing Escherichia coli O157:H7 on apples. “Red delicious” apples inoculated with a 5 strain mixture E. were subjected to washing in sterile deionized water containing 0% PDA (control), 0.15% TC, 0.35% CR, 0.30% 0.5% BR, or 1% BR 1, 3, min at 23 °C presence absence soil, surviving...
Clostridium difficile is an anaerobic sporeforming pathogen causing a toxin-mediated enteric disease in humans. C. predominantly affects hospital inpatients undergoing prolonged antibiotic therapy, which results dysbiosis, leading to spore germination, colonization the intestine and subsequent toxin production. Therapeutic agents that inhibit without dysbiosis could improve clinical outcome of infections. Our previous studies indicated carvacrol (CR), plant-derived compound significantly...
Listeria monocytogenes is a human enteric pathogen that causes severe foodborne illness in high-risk populations. Crossing the intestinal barrier first critical step for infection. Therefore, reducing L. colonization and invasion of epithelium production virulence factors could potentially control listeriosis humans. This study investigated efficacy sub-inhibitory concentration (SIC) plant-derived antimicrobial eugenol, either alone, or combination with five lactic acid bacteria (LAB),...
Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of ruminant enteritis, targets intestinal macrophages. During infection, macrophages contribute to mucosal inflammation and development granulomas in small intestine which worsens as disease progression occurs. Vitamin D3 is an immunomodulatory steroid hormone with beneficial roles host-pathogen interactions. Few studies have investigated immunologic 25-hydroxyvitamin (25(OH)D3) 1,25-dihydroxyvitamin (1,25(OH)2D3)...
Urinary tract infections (UTIs) in the United States result more than 7 million hospital visits per year. Uropathogenic Escherichia coli (UPEC) is responsible for 80% of UTIs. Although antibiotics are drug choice to control UTIs, their repeated use has resulted emergence antibiotic-resistant UPEC. Thus, there a need effective alternate strategies UPEC infections. This study investigated efficacy trans-cinnamaldehyde (TC), food-grade molecule present cinnamon, reducing colonization and...
Purpose. Clostridium difficile is an anaerobic spore-forming pathogen that causes a serious toxin-mediated enteric disease in humans. Therapeutic agents are capable of reducing C. spore production could significantly minimize the transmission and relapse infections. This study investigated efficacy food-grade, plant-derived compound, carvacrol (CR), production, germination outgrowth. Methodology. Two hyper-virulent isolates (ATCC BAA 1870 1805) were grown with or without sub-inhibitory...
This study investigated the prophylactic and therapeutic efficacies of baicalin (BC), a plant-derived flavone glycoside, in reducing severity Clostridioides difficile infection (CDI) mouse model. In trial, C57BL/6 mice were provided with BC (0, 11, 22 mg/L drinking water) from 12 days before C. challenge through end experiment, whereas administration started day 1 post trial. Both control groups infected 106 CFU/mL hypervirulent BAA 1803 spores or sterile PBS, clinical diarrheal scores...
Purpose. This study investigated the efficacy of essential mineral, selenium (sodium selenite), in reducing toxin production, spore outgrowth and antibiotic resistance Clostridium difficile vitro. Methodology. Two hypervirulent C. isolates were cultured brain heart infusion broth with without a sub-minimum inhibitory concentration (sub-MIC) sodium selenite, supernatant bacterial pellet harvested for total quantitation RT-qPCR analysis toxin-encoding genes, respectively. Additionally,...
While Clostridioides difficile is recognized as an important human pathogen, it also a significant cause of gastroenteritis and associated diarrhea in neonatal pigs. Since clinical disease rarely diagnosed piglets older than 1 week age, hypothesized that natural resistance with the increased complexity intestinal microbiota animals age. To test this, were challenged C. (ribotype 078/toxinotype V) at times ranging from 2 to 14 days severity microbial diversity cecal assessed. Half 4 age...
The role of vitamin D3 in modulating immune responses has been well-established for over two decades; however, its specific functions have not extensively detailed cattle, particularly cattle different stages infection with Mycobacterium avium subspecies paratuberculosis (MAP). Consistent previous work our lab, the present study showed that infected clinical stage disease reduced serum 25-hydroxyvitamin [25(OH)D3]. Additionally, effects on peripheral blood mononuclear cells (PBMCs) from...
Vibrio cholerae is a water-borne pathogen responsible for causing toxin-mediated profuse diarrhea in humans, leading to severe dehydration and death unattended patients. With increasing reports of antibiotic resistance V. cholerae, there need alternate interventional strategies controlling cholera. A potential new strategy treating infectious diseases involves targeting bacterial virulence rather than growth, where pathogen's specific mechanisms critical infection hosts are inhibited. Since...