A5101844007- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Wheat and Barley Genetics and Pathology
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
- Plant Virus Research Studies
- Hops Chemistry and Applications
- Plant Pathogens and Resistance
- Plant Genetic and Mutation Studies
- Genetics and Plant Breeding
- Plant pathogens and resistance mechanisms
- Genomic variations and chromosomal abnormalities
- Mycotoxins in Agriculture and Food
- CRISPR and Genetic Engineering
- Bioenergy crop production and management
- Fungal Plant Pathogen Control
- Weed Control and Herbicide Applications
- Plant Pathogens and Fungal Diseases
- Silicon Effects in Agriculture
- Soybean genetics and cultivation
- Rice Cultivation and Yield Improvement
- Crop Yield and Soil Fertility
- Animal Genetics and Reproduction
- Powdery Mildew Fungal Diseases
- Aluminum toxicity and tolerance in plants and animals
University of Nebraska–Lincoln
2025
North Dakota State University
2022-2025
Agricultural Research Service
2022-2023
Kansas State University
2012-2019
University of Missouri
2007-2019
Russian State Agrarian University - Moscow Timiryazev Agricultural Academy
2003-2007
Genome structure variation, including copy number variation and presence/absence comprises a large extent of maize genetic diversity; however, its effect on phenotypes remains largely unexplored. Here, we describe how underlies rare allele that contributes to aluminum (Al) tolerance. Al toxicity is the primary limitation for crop production acid soils, which make up 50% world's potentially arable lands. In recombinant inbred line mapping population, tolerance gene multidrug toxic compound...
Recent rapid evolution and spread of resistance to the most extensively used herbicide, glyphosate, is a major threat global crop production. Genetic mechanisms by which weeds evolve herbicides largely determine level rate resistance. In previous study, we determined that glyphosate in Kochia scoparia due amplification 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) gene, enzyme target glyphosate. Here, investigated genomic organization amplified EPSPS copies using fluorescence situ...
Significance Whole-chromosome oligo-FISH paints using synthetic oligonucleotide libraries that can be amplified and labeled were generated for all 10 chromosomes of maize, facilitating chromosome studies with high sensitivity specificity genetically diverse lines. Applications include visualization simple or complex chromosomal aberrations, establishment domains, illustration mitotic meiotic behavior, providing insights into relationships.
A cytogenetic map of wheat was constructed using FISH with cDNA probes. markers detected homoeology and chromosomal rearrangements wild relatives, an important source genes for improvement. To transfer agronomically from relatives to bread (Triticum aestivum L., 2n = 6 x 42, AABBDD) by induced homoeologous recombination, it is know the relationships species involved. Fluorescence in situ hybridization (FISH) can be used study chromosome structure. The genomes allohexaploid other Triticeae...
Maize is a highly diverse species on the gene sequence level. With recent development of methods to distinguish each 10 pairs homologues in somatic root tip spreads, wide collection maize lines was subjected karyotype analysis serve as reference for community and examine spectrum chromosomal features species. The core nested association mapping progenitor additional selections diversity were examined. Commonly used inbred included analysis. centromere 4 specific repeat ribosomal RNA loci...
Fluorescence in situ hybridization with probes for 45 cDNAs and five tandem repeats revealed homoeologous relationships of Agropyron cristatum wheat. The results will contribute to alien gene introgression wheat improvement. Crested wheatgrass (Agropyron L. Gaertn.) is a wild relative promising source novel genes To date, identification A. chromosomes has not been possible, its molecular karyotype available. Furthermore, relationship between the genomes determined. develop...
Recent and rapid evolution of resistance to glyphosate, the most widely used herbicides, in several weed species, including common waterhemp (Amaranthus tuberculatus), poses a serious threat sustained crop production. We report that glyphosate A tuberculatus was due amplification 5-enolpyruvylshikimate-3-P synthase (EPSPS) gene, which encodes molecular target glyphosate. There positive correlation between EPSPS gene copies its transcript expression. analyzed distribution genome using...
Combined with a system for identifying each of the chromosomes in genome, visualizing location individual genetic loci by fluorescence situ hybridization (FISH) would aid assembling physical and maps. Previously, large genomic clones have been successfully used as FISH probes onto somatic but this approach is complicated species abundant repetitive elements. In study, repeat-free portions sequences that were anchored to particular including genes, gene clusters, cDNAs, BACs obtained from...
During evolutionary history many grasses from the tribe Triticeae have undergone interspecific hybridization, resulting in allopolyploidy; whereas homoploid hybrid speciation was found only rye. Homoeologous chromosomes within preserved cross-species macrocolinearity, except for a few species with rearranged genomes. Aegilops markgrafii, diploid wild relative of wheat (2n = 2x 14), has highly asymmetrical karyotype that is indicative chromosome rearrangements. Molecular cytogenetics and...
Barbara McClintock reported that the Ac/Ds transposable element system can generate major chromosomal rearrangements (MCRs), but underlying mechanism has not been determined. Here, we identified a series of chromosome derived from maize lines containing pairs closely linked Ac termini. Molecular and cytogenetic analyses showed MCRs in these comprised 17 reciprocal translocations two large inversions. The breakpoints all 19 are delineated by termini characteristic 8-base-pair target site...
Summary Next‐generation sequencing ( NGS ) provides a powerful tool for the discovery of important genes and alleles in crop plants their wild relatives. Despite great advances technologies, whole‐genome shotgun is cost‐prohibitive species with complex genomes. An attractive option to reduce genome complexity single chromosome prior sequencing. This work describes strategy studying genomes distant relatives wheat by isolating chromosomes from addition or substitution lines, followed sorting...
Aegilops speltoides (2n=2x=14, genome SS) is a wild relative of wheat and donor useful traits for improvement. Several whole-genome studies compared genic regions from the Sitopsis section found that Ae. most closely related to B subgenome but not its direct progenitor. The results showed ancestor diverged more than 4 MYA either has yet been discovered, or extinct. To further explore evolutionary relationship between develop chromosome paints, we performed comparative analysis repetitive...
Goss's wilt (GW) of maize is caused by the Gram-positive bacterium Clavibacter michiganensis subsp. nebraskensis (Cmn) and has spread in recent years throughout Great Plains, posing a threat to production. The genetic basis plant resistance unknown. Here, simple method for quantifying disease symptoms was developed used select cohorts highly resistant susceptible lines known as extreme phenotypes (XP). Copy number variation (CNV) analyses using whole genome sequences bulked XP revealed 141...