A5088869451- Advanced MRI Techniques and Applications
- Ultrasound and Hyperthermia Applications
- Infrared Thermography in Medicine
- Electromagnetic Fields and Biological Effects
- Atomic and Subatomic Physics Research
- Transcranial Magnetic Stimulation Studies
- Neurological disorders and treatments
- Radiation Dose and Imaging
- Advanced Biosensing Techniques and Applications
- Chronic Myeloid Leukemia Treatments
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Neuroscience and Neural Engineering
- Fault Detection and Control Systems
- Quality and Safety in Healthcare
- Protein Interaction Studies and Fluorescence Analysis
- Ubiquitin and proteasome pathways
- EEG and Brain-Computer Interfaces
- Nuclear Physics and Applications
- CCD and CMOS Imaging Sensors
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Biofield Effects and Biophysics
- Acute Lymphoblastic Leukemia research
- CAR-T cell therapy research
Massachusetts General Hospital
2025
The Ohio State University Wexner Medical Center
2024
Miltenyi Biotec (Germany)
2019-2022
Heinrich Heine University Düsseldorf
2014-2016
Düsseldorf University Hospital
2014
Medizinische Hochschule Hannover
2006-2007
University of Bonn
2007
General Electric (United States)
1986-2006
Applied Sciences Laboratory (United States)
2000
Cedars-Sinai Medical Center
1989-1990
Micro RNAs (miRNA) regulate gene expression by hybridization and recruitment of multi-protein complexes to complementary mRNA target sequences. miRNA function can transiently be antagonized antagomirs-chemically modified oligonucleotides individual miRNAs. Here, we describe the induction stable loss-of-function phenotypes for specific miRNAs lentivirus-mediated antagomir expression. Lentivirally expressed antagomirs are transcribed from a H1-promoter located within lentiviral 3'LTR were...
The neural cell adhesion molecule NCAM plays an important role during development and in the adult brain. To study intracellular trafficking of neurons, two major isoforms, NCAM140 or NCAM180, were expressed primary cortical neurons rat B35 neuroblastoma line. was endocytosed subsequently recycled to plasma membrane, whereas only a minor fraction degraded lysosomes. In endocytosis detected soma, neurites growth cones developmentally regulated fashion. Furthermore, we found that is...
Peripheral nerve stimulation (PNS) caused by time-varying magnetic fields has been studied both theoretically and experimentally. A human volunteer study performed on three different body-size gradient coils one head-size coil is presented in this work. The experimental results were used to generate average PNS threshold parameters for the tested systems. It was found that increases while gradient-region-of-uniformity size decreases. In addition, linear relationships between diameter of...
Increases in tissue temperature caused by exposure to radiofrequency (RF) radiation are a primary safety concern of magnetic resonance imaging (MRI). Therefore, body and skin temperatures were measured six subjects before (20 min), during (30 min) after MRI procedures performed at specific absorption rates (SARs) 10 times higher than the limit recommended UK National Radiological Protection Board. Body was unchanged throughout experiment. Abdominal increased significantly (p less 0.05) MRI,...
PURPOSE: To assess the physiologic responses to a magnetic resonance (MR) procedure performed at whole-body-averaged specific absorption rate (SAR) of 6.0 W/kg. MATERIALS AND METHODS: Tympanic membrane temperature, skin temperature seven sites, heart rate, blood pressure, oxygen saturation, and cutaneous flow in six volunteers were assessed before, for 16 minutes during, immediately after MR imaging 1.5 T, 64 MHz. RESULTS: Statistically significant (P < .05) increases tympanic chest,...
Solid tumors consist of malignant and nonmalignant cells that together create the local tumor microenvironment (TME). Additionally, TME is characterized by expression numerous soluble factors such as TGF-β. TGF-β plays an important role in suppressing T cell effector function promoting invasiveness. Up to now CAR exclusively target tumor-associated antigens (TAA) located on membrane. Thus, strategies exploit targets within are needed. This study demonstrates a novel approach using Adapter...
Superficial- and deep-tissue heating was measured in five dogs during high-specific-absorption-rate radiofrequency (RF) irradiation to see whether significant temperature changes could be produced by a 1.5-T clinical magnetic resonance imager. The RF power output employed 6.3 times that required for routine imaging. Temperature probes were placed both deep superficial tissues, temperatures recorded before, during, after exposure. In each dog, there linear increase of several degrees...
Abstract Rat brain was exposed to 591‐MHz, continuous‐wave (CW) microwaves at 13.8 or 5.0 mW/cm 2 determine the effect on nicotinamide adenine dinucleotide, reduced (NADH), adenosine triphosphate (ATP) and creatine phosphate (CP) levels. On initiation of in vivo microwave exposures, fluorimetrically determined NADH rapidly increased a maximum 4.0%–12.5% above pre‐exposure control levels one‐half minute, then decreased slowly 2% three minutes, finally increasing 5% level five minutes. ATP CP...
Abstract Purpose: To compare thresholds for peripheral nerve stimulation from gradient switching in whole body magnetic resonance (MR) equipment of different design. Materials and Methods: Threshold data obtained three experiments were reformatted into a single joint set describing anterio‐posterior (AP) orientation Echo Planar Imaging (EPI) waveforms with bipolar ramp times between 0.07 1.2 ms. Reformatting included the use of: a) rate change maximum field patient space as measure output,...
Thermal effects of MR imaging: worst-case studies on sheep.B J Barber, D Schaefer, C Gordon, Zawieja and HeckerAudio Available | Share
Reports in the literature concerning effect of static magnetic fields on body temperature mammals have been contradictory and confusing. A significant increase human subjects exposed to used resonance imaging (MRI) would important safety implications. Therefore, two separate studies we determined 20 a 1.5 T field. One group (Group I, N = 9) had sublingual pocket measured immediately before after 60 min exposure, while another II, 11) esophageal at 2 intervals during exposure. No...
Abstract The literature has conflicting reports concerning the effect of static magnetic fields on body and skin temperatures in mammals. Since temperature changes induced by would have important safety implications for clinical resonance imaging, (sublingual pocket) (abdomen, forehead, chest, upper arm, forearm, thigh, calf) were determined six normal subjects using a fluoroptic thermometry system during 20‐min exposure to 1.5‐T field. Ambient conditions controlled held constant. An...
In magnetic (eddy-current) stimulation, sensory and motor nerve are stimulated by a time-varying field (dB/dt) produced in an appropriate coil. this study, stimulation thresholds for solenoidal coil encircling the forearm were determined 14 healthy human volunteers, 11 males 3 females. Damped sinusoidal dB/dt pulses with durations (onset to zero crossing) ranging from 120 /spl mu/s 2500 discharging capacitor bank into Rectangular 50 generated Gradient Ramp Accelerator Module (GRAM) series...
In magnetic resonance, time-varying gradient fields (dB/dt) may stimulate nerves or muscles by inducing electric in patients. Models predicted mean peripheral nerve and cardiac stimulation thresholds. For ramp durations of less than a few milliseconds, is safe indicator high dB/dt. At sufficient amplitudes, perceptible (ie, tingling tapping sensations). Magnetic from simultaneous axes combine almost as vector sum to produce stimulation. Patients become uncomfortable at amplitudes 50%–100%...
Here we present a novel method "Genomic inverse PCR for exploration of ligated breakpoints" (GIPFEL) that allows the sensitive detection recurrent chromosomal translocations. This technique utilizes limited amounts DNA as starting material and relies on based quantification unique sequences are created by circular ligation restricted genomic from translocation bearing cells. Because complete potential breakpoint region is interrogated, prior knowledge individual, specific interchromosomal...
The eddy-current distribution in a 3-D inhomogeneous model of dog's thorax due to time varying fields magnetic resonance imaging (MRI) x, y, and z-gradient coils pair coplanar are calculated. From experimental data cardiac threshold from coil for 540 mu s rectangular pulse, current density 21.5 A/m/sup 2/ was For co-planar pair, the stimulation is 19.2 at duration 640 s. different conductivities tissues have significant influence on flow. Calculations combined with measurements will help...