A5030909057- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Nicotinic Acetylcholine Receptors Study
- Fish biology, ecology, and behavior
- Amyotrophic Lateral Sclerosis Research
- Aquaculture Nutrition and Growth
- Nerve injury and regeneration
- Reproductive biology and impacts on aquatic species
- Memory and Neural Mechanisms
- Cardiac electrophysiology and arrhythmias
- Physiological and biochemical adaptations
- RNA Research and Splicing
- Biochemical Analysis and Sensing Techniques
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- S100 Proteins and Annexins
- Olfactory and Sensory Function Studies
- RNA and protein synthesis mechanisms
- Neuroscience and Neural Engineering
- Ichthyology and Marine Biology
University of Toronto
2015-2020
Stanford University
2020
Queen's University
2014
Significance Huntington’s disease (HD) is a fatal neurodegenerative disorder that currently has no cure. Although HD classically considered motor disorder, patients experience learning and memory deficits years before the onset of symptoms, these resemble those observed in mouse models. In this work, using transgenic models HD, we demonstrate action neurotransmitter GABA switched from inhibitory to excitatory. By treating mice with clinically used diuretic (bumetanide), which restores GABA,...
KCC2 is a neuron-specific K+-Cl– cotransporter essential for establishing the Cl- gradient required hyperpolarizing inhibition in central nervous system (CNS). highly localized to excitatory synapses where it regulates spine morphogenesis and AMPA receptor confinement. Aberrant function contributes human neurological disorders including epilepsy neuropathic pain. Using functional proteomics, we identified KCC2-interactome mouse brain determine KCC2-protein interactions that regulate...
Abstract Olfactory processing is thought to be actively modulated by the top-down input from cortical regions, but behavioural function of these signals remains unclear. Here we find that feedback anterior olfactory nucleus pars medialis (mAON) bidirectionally modulates sensitivity and olfaction-dependent behaviours. To identify a limbic tunes this mAON switch, further demonstrate optogenetic stimulation ventral hippocampal inputs sufficient alter
Abstract Amyotrophic lateral sclerosis is a fatal disease resulting from motor neuron degeneration in the cortex and spinal cord. Cortical hyperexcitability hallmark feature of amyotrophic accompanied by decreased intracortical inhibition. Using electrophysiological patch-clamp recordings, we revealed parvalbumin interneurons to be hypoactive late pre-symptomatic SOD1*G93A mouse model sclerosis. We discovered that using adeno-associated virus-mediated delivery chemogenetic technology...
Voltage-gated sodium channels regulate neuronal excitability and fast synaptic transmission in the postnatal adult brain. The gene SCN2A, encoding channel Nav1.2, regulates development variants SCN2A are associated with autism spectrum disorders (ASD). expression pattern of begins during fetal cortical development, prior to onset transmission, but it is unknown whether early through mechanisms independent transmission. Here we reveal that isogenic ASD patient-derived human forebrain...
Synaptic inhibition depends on a transmembrane gradient of chloride, which is set by the neuron-specific K+-Cl− co-transporter KCC2. Reduced KCC2 levels in neuronal membrane contribute to generation epilepsy, neuropathic pain, and autism spectrum disorders; thus, it important characterize mechanisms regulating expression. In present study, we determined role KCC2-protein interactions total surface Using quantitative immunofluorescence cultured mouse hippocampal neurons, discovered that...
Neto2 is a transmembrane protein that interacts with the neuron-specific K(+)-Cl(-) cotransporter (KCC2) in central nervous system (CNS). Efficient KCC2 transport essential for setting neuronal Cl(-) gradient, which required fast GABAergic inhibition. to maintain normal abundance of neurons, and increases function by binding active oligomeric form this cotransporter. In present study, we characterized inhibition KCC2-mediated chloride homeostasis pyramidal neurons from adult hippocampal...
At tripartite synapses, astrocytes enmesh synaptic contacts, but how contribute to the formation, maturation and plasticity of synapses remains elusive. Here we show that both neurons abundantly express neurexin-1, a presynaptic adhesion molecule controls properties. Using super-resolution imaging, demonstrate neuronal astrocytic neurexin-1 form discrete nanoclusters at excitatory synapses. We find distinct patterns heparan sulfate modification alternative splicing confer onto different...
In neuroendocrine cells, hormone release often requires a collective burst of action potentials synchronized by gap junctions. This is the case for electrically coupled bag cell neurons in reproductive system marine snail, Aplysia californica. These cells are found two clusters, and fire synchronous burst, called afterdischarge, resulting neuropeptide secretion triggering ovulation. However, physiology pharmacology neuron electrical synapse not completely understood. As such, we made dual...
Polarization sensitivity (PS) in vertebrate vision is controversial, perhaps because its underlying mechanism has remained obscure. An issue that might have added to the controversy rainbow trout ( Oncorhynchus mykiss ), which served as primary model system for polarization-based orientation, lose their ability orient relative celestial polarized-light patterns when parr (fry) transform into migratory smolts (juveniles), would benefit most from orientation. Here we addressed two key...
Electrical transmission is a dynamically regulated form of communication and key to synchronizing neuronal activity. The bag cell neurons Aplysia are group electrically coupled neuroendocrine cells that initiate ovulation by secreting egg-laying hormone during prolonged period synchronous firing called the afterdischarge. Accompanying afterdischarge an increase in intracellular Ca2+ activation protein kinase C (PKC). We used whole recording from paired cultured demonstrate electrical...
Abstract KCC2 is a neuron-specific K + -Cl − cotransporter essential for establishing the Cl gradient required hyperpolarizing inhibition. highly localized to excitatory synapses where it regulates spine morphogenesis and AMPA receptor confinement. Aberrant function contributes numerous human neurological disorders including epilepsy neuropathic pain. Using unbiased functional proteomics, we identified KCC2-interactome in mouse brain determine KCC2-protein interactions that regulate...