A5074063013- Neonatal Respiratory Health Research
- Renal and related cancers
- Energy Harvesting in Wireless Networks
- Congenital Diaphragmatic Hernia Studies
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Extracellular vesicles in disease
- Dental Research and COVID-19
- RNA Interference and Gene Delivery
- Neurological and metabolic disorders
- Respiratory viral infections research
- CAR-T cell therapy research
- Bacteriophages and microbial interactions
- SARS-CoV-2 detection and testing
- Cardiovascular Syncope and Autonomic Disorders
- Mosquito-borne diseases and control
- Burkholderia infections and melioidosis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Congenital heart defects research
- Cystic Fibrosis Research Advances
- COVID-19 diagnosis using AI
- Sepsis Diagnosis and Treatment
- Transplantation: Methods and Outcomes
- Cardiovascular Function and Risk Factors
- Medical Imaging and Pathology Studies
Pulmonary and Allergy Associates
2024
University of California, San Francisco
2024
Malankara Orthodox Syrian Church Medical College Hospital
2021-2024
Boston University
2016-2020
Boston Medical Center
2017-2020
Center for Neuroscience and Regenerative Medicine
2017
It has been postulated that during human fetal development, all cells of the lung epithelium derive from embryonic, endodermal, NK2 homeobox 1-expressing (NKX2-1+) precursor cells. However, this hypothesis not formally tested owing to an inability purify or track these progenitors for detailed characterization. Here we have engineered and developmentally differentiated NKX2-1GFP reporter pluripotent stem (PSCs) in vitro generate isolate primordial express NKX2-1 but are initially devoid...
Abstract Efficient generation of human induced pluripotent stem cell (hiPSC)-derived intestinal organoids (HIOs) would facilitate the development in vitro models for a variety diseases that affect gastrointestinal tract, such as inflammatory bowel disease or Cystic Fibrosis. Here, we report directed differentiation protocol mesenchyme-free HIOs can be primed towards more colonic proximal lineages serum-free defined conditions. Using CDX2 eGFP iPSC knock-in reporter line to track emergence...
The in vitro directed differentiation of pluripotent stem cells (PSCs) through stimulation developmental signaling pathways can generate mature somatic cell types for basic laboratory studies or regenerative therapies. However, there has been significant uncertainty regarding how to separately derive lung vs. thyroid epithelial lineages, since these two each originate from Nkx2-1+ foregut progenitors, and the minimal claimed regulate their distinct lineage specification vivo have varied...
Lung epithelial lineages have been difficult to maintain in pure form vitro, and lineage-specific reporters proven invaluable for monitoring their emergence from cultured pluripotent stem cells (PSCs). However, reporter constructs tracking proximal airway generated PSCs not previously available, limiting the characterization of these cells. Here, we engineer mouse human PSC lines carrying secretory lineage that facilitate tracking, purification, profiling this lung subtype. Through bulk...
Regenerative medicine techniques are being widely investigated for organs without regenerative capacity, such as lung. Whether pluripotent stem cell derived thyroid organoids will be used in the future a approach to treat hypothyroidism is unclear. In context of an easily available, very cheap and non-invasive substitutive therapy with levothyroxine, cost-risk balance clearly speaks against novel approach. However, pneumocyte follicular precursors share key transcription factor, NKX2-1,...
Melioidosis, an emerging infectious disease in India is well known for its protean clinical manifestations. Definitive diagnosis can be made by isolation of non-fermentative Gram-negative bacilli from specimens and identification the isolate at species level. We report a case disseminated melioidosis with lymphadenitis prostatic abscess. Burkholderia pseudomallei was isolated bone marrow, pus aspirate, blood urine, patient responded to treatment.
Respiratory specimens collected via nasopharyngeal and throat swabs are the recommended method of choice for molecular detection Influenza A virus. However, they often cause discomfort to patients require trained healthcare workers. This study aimed validate a cost-effective nucleic acid extraction-free PCR using SYBR-Green chemistry with saliva samples diagnose respiratory illnesses caused by viruses. enrolled symptomatic pediatric adult influenza-like illness. based amplification test was...
Introduction. COVID-19 caused by SARS CoV-2 continues to be a major health concern globally. Methods for detection of the disease are necessary public efforts monitor spread this as well detecting emergence new variants.Gap statement. Collection Nasopharyngeal swab (NPS), gold standard sample infection RT-qPCR is invasive and requires expertise trained medical provider. This highlights need validating less samples that can self-collected without provider.Aim. To validate saliva tongue...
Background: Scrub typhus (ST) is a common Rickettsial infection which has been increasingly reported from the various states of southern part India.
 Aims and Objective: With very few reports Central Kerala, we aimed to study seroprevalence scrub its clinical profile.
 Materials Methods: Patients presenting with acute undifferentiated febrile illness other symptoms signs infections during period two years were included in this prospective study. Serodiagnosis ST was based on...
<b>Introduction</b> Alpha-1 antitrypsin (AAT) deficiency (AATD) is the most frequent form of genetically determined emphysema. Type II alveolar cells (AEC2) are capable producing AAT but little known about their role in AATD pathogenesis. <b>Aims and objectives</b> We hypothesise that AEC2s play an intrinsic pathogenesis therefore we sought to engineer a novel <i>in vitro</i> model enable derivation lung epithelial define developmental timing expression emerging AEC2s. <b>Methods</b>...
<h3>Background</h3> Cystic fibrosis (CF) is one of the most common lethal genetic diseases and caused by mutations in CFTR gene where a deletion phenylalanine at position 508 (F508del) accounts for prevalent mutation worldwide. MicroRNA (miRNA) are small non-coding RNAs that can regulate up to two thirds expression human cells have been shown be dysregulated CF airway implicated direct control Induced Pluripotent stem (iPSC) routinely generated from any patient contain person's unique...
Abstract Alveolar epithelial type 2 cells (AEC2s) are the facultative progenitors responsible for maintaining lung alveoli throughout life, yet difficult to access from patients biomedical research or regeneration applications. Here we engineer AEC2s human induced pluripotent stem (iPSCs) in vitro and use single cell RNA sequencing (scRNA-seq) profile detailed kinetics of their differentiation over time. We focus on both desired target as well those that appear diverge alternative endodermal...
Background: Procalcitonin (PCT) was found to be a valuable and reliable biomarker for sepsis, especially in critical care patients whom early recognition prompt treatment could reduce mortality. Aims Objectives: This study aimed at correlating the levels of PCT as diagnostic marker sepsis relation culture positivity various samples from blood, respiratory, urine, exudates admitted tertiary hospital. Materials Methods: Results level along with bacterial results were analyzed 780 period 1...