A5033490245- Vector-borne infectious diseases
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Vector-Borne Animal Diseases
- COVID-19 Clinical Research Studies
- Cancer Cells and Metastasis
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Dengue and Mosquito Control Research
- Pluripotent Stem Cells Research
- Viral Infections and Immunology Research
- Pancreatitis Pathology and Treatment
- Renal and related cancers
- Long-Term Effects of COVID-19
- Insect and Pesticide Research
- Invertebrate Immune Response Mechanisms
- Antimicrobial Peptides and Activities
- COVID-19 epidemiological studies
- Glycosylation and Glycoproteins Research
- Influenza Virus Research Studies
Century Therapeutics (United States)
2024
Boston Medical Center
2017-2022
Boston University
2017-2022
University Medical Center
2022
The University of Texas Medical Branch at Galveston
2012-2014
Abstract Efficient generation of human induced pluripotent stem cell (hiPSC)-derived intestinal organoids (HIOs) would facilitate the development in vitro models for a variety diseases that affect gastrointestinal tract, such as inflammatory bowel disease or Cystic Fibrosis. Here, we report directed differentiation protocol mesenchyme-free HIOs can be primed towards more colonic proximal lineages serum-free defined conditions. Using CDX2 eGFP iPSC knock-in reporter line to track emergence...
ABSTRACT Tick-borne flaviviruses (TBF) are widely dispersed across Africa, Europe, Asia, Oceania, and North America, some present a significant threat to human health. Seminal studies on tick-borne encephalitis viruses (TBEV), based partial envelope gene sequences, predicted westward clinal pattern of evolution dispersal northern Eurasia, terminating in the British Isles. We tested this hypothesis using all available full-length open reading frame (ORF) TBF sequences. Phylogenetic analysis...
Ixodes species ticks are competent vectors of tick-borne viruses including encephalitis and Powassan encephalitis. Tick saliva has been shown to facilitate enhance viral infection. This likely occurs by saliva-mediated modulation host responses into patterns favorable for infection dissemination. Because the rapid kinetics transmission, this must occur as early tick attachment initiation feeding. In study, cutaneous bite-site lesions were analyzed using Affymetrix mouse genome 430A 2.0...
Gam-COVID-Vac (SPUTNIK V) has been granted emergency use authorization in 70 nations and administered to millions worldwide. However, there are very few peer-reviewed studies describing its effects. Independent reports regarding safety effectiveness could accelerate the final approval by WHO. We aimed study long-term humoral immune response naïve previously infected volunteers who received SPUTNIK V.Humoral responses, assayed anti-SARS-CoV-2-spike-RBD IgG ELISA neutralization assays, were...
Abstract Background Ixodes scapularis ticks are hematophagous arthropods capable of transmitting many infectious agents to humans. The process blood feeding is an extended and continuous interplay between tick host responses. While this has been studied extensively in vitro , no global understanding the response emerged. Methods To address issue, we used PCR-arrays measure skin-specific expression 233 discrete genes at 8 time points during primary secondary infestations mice with...
Emerging and re-emerging diseases transmitted by blood feeding arthropods are significant global public health problems. Ticks transmit the greatest variety of pathogenic microorganisms any arthropod. Infectious agents ticks delivered to vertebrate host together with saliva at bite site. Tick salivary glands produce complex cocktails bioactive molecules that facilitate pathogen transmission modulating hemostasis, pain/itch responses, wound healing, both innate adaptive immunity. In this...
Tick salivary glands produce complex cocktails of bioactive molecules that facilitate blood feeding and pathogen transmission by modulating host hemostasis, pain/itch responses, wound healing, both innate adaptive immunity. In this study, cutaneous responses at Dermacentor andersoni bite-sites were analyzed using Affymetrix mouse genome arrays histopathology 12, 48, 96 120 h post- infestation (hpi) during primary infestations hpi secondary infestations. The microarray data suggests: (1)...
Abstract We hypothesized that in individuals with previous SARS-CoV-2 infection, the first vaccine dose would work as a booster, eliciting faster and more intense immune response. herein describe antibody responses to second doses of Gam-COVID-Vac (SPUTNIK V) health personnel Tucumán, Argentina, COVID-19 compared it uninfected personnel. Individuals anti-SARS-CoV-2 titers at baseline showed significantly higher than people no prior history disease (p <0.0001), those registered after...
Dear Editor, In September 2012, we collected ticks from Missouri with the goal of isolating novel phlebovirus, Heartland virus (HRTV). HRTV was described in two farmers northwestern who presented thrombocytopenia and severe febrile illness.1 These patients were both bitten by 5–7 days before onset their clinical symptoms. Our hypothesis that would isolate inoculation cell culture and/or detection viral RNA on polymerase chain reaction (PCR) assay. Furthermore, used this field surveillance...
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused a global pandemic with dramatic health and socioeconomic consequences. Coronavirus Disease 2019 (COVID-19) challenges systems to quickly respond by developing new diagnostic strategies that contribute identify infected individuals, monitor infections, perform contact-tracing, limit the spread of virus. In this brief report, we developed highly sensitive, specific, precise "In-House" ELISA correctly discriminate...
Background: Gam-COVID-Vac, also known as SPUTNIK V, is the first COVID-19 vaccine registered, has emergency use authorization in 70 nations, and been administered to millions worldwide. However, there are very few peer-reviewed studies describing its effects. As independent reports begin be published, a more accurate picture regarding safety effectiveness could accelerate approval by WHO.Methods: An ELISA that detects anti-SARS-CoV-2-spike-RBD IgG was used, both transversal longitudinal...