Small interfering RNAs offer novel opportunities to inhibit gene expression in a highly selective and efficient manner but depend on cytosolic translocation with synthetic delivery systems. The polyethylenimine (PEI) is widely used for plasmid DNA transfection. However, the water-soluble PEI does not form siRNA polyplexes stable enough extracellular media effective delivery. We previously showed that rendering insoluble physiological media, without modifying drastically its overall cationic...

Snake venom and mammalian secreted phospholipases A₂ (sPLA₂s) have been associated with toxic (neurotoxicity, myotoxicity, etc.), pathological (inflammation, cancer, physiological (proliferation, contraction, secretion, etc.) processes. Specific membrane receptors (M N types) for sPLA₂s initially identified snake as ligands, the M-type 180-kDa receptor was cloned from different animal species. This paper addresses problem of endogenous ligands receptor....

Screening for EGFR mutation is a key molecular test management of lung cancer patients. Outcome patients with receiving tyrosine kinase inhibitor known to be better across different ethnic populations. However, frequency mutations and the clinical response in most other populations, including India, remains explored. We conducted retrospective analysis Indian who were managed oral inhibitors. Majority study had adenocarcinoma non-smokers. 39/111 tested positive domain determined by Taqman...

Oral cancers and other squamous cell of the head neck are common in India, primarily due to tobacco chewing/smoking alcohol consumption. Recent reports indicate involvement human papillomavirus (HPV), HPV 16, a subset carcinoma (SCCHN) cases. To investigate types HPVs present 83 oral 19 tumors, degenerate primers directed consensus regions L1 open reading frame (ORF) were employed amplify genomic DNA from tumor when available, adjacent normal mucosa. PCR-amplified products cloned sequenced....

Human papilloma virus (HPV) accounts for the most common cause of all virus-associated human cancers. Here, we describe first graphic user interface (GUI)-based automated tool 'HPVDetector', non-computational biologists, exclusively detection and annotation HPV genome based on next-generation sequencing data sets. We developed a custom-made reference that comprises chromosomes along with annotated 143 types as pseudochromosomes. The runs dual mode defined by user: 'quick mode' to identify...

Abstract Background Survivin, an inhibitor of apoptosis, is overexpressed in cancer. It has been implicated both prevention apoptosis and cell cycle regulation. We investigated the distribution antiapoptotic protein survivin 29 oral squamous carcinoma (OSCC) 16 premalignant lesions. suggested that wild‐type p53 represses expression. Therefore, we status relation to determine potential involvement tumorigenesis. Methods Oral cancer tissues were freshly obtained at time surgery classified as...

Persistent HPV infection plays a major role in cervical cancer. This study was undertaken to identify types cohort of Indian women with locally advanced cancer as well determine the physical state and/or site viral integration host genome. Pretreatment biopsies (n = 270) from patients were screened for by high throughput genotyping assay based on luminex xMAP technology MY09/11 PCR and SPF1/2 PCR. Overall positivity observed be 95%, HPV16 being most common (63%) followed HPV18. Integration...

Abstract Cervical cancer is the second most common among women worldwide, with developing countries accounting for >80% of disease burden. Although in West, active screening has been instrumental reducing incidence cervical cancer, management hampered due to lack biomarkers progression and defined therapeutic targets. Here we carried out gene expression profiling 29 tissues from I ndian women, spanning International Federation Gynaecology Obstetrics ( FIGO ) stages early lesion (IA IIA)...

Successful cytosolic delivery enables opportunities for improved treatment of various genetic disorders, infectious diseases and cancer. Cationic nanoemulsions were designed using alternative excipients evaluated particle size, charge, effect sterilization on its stability, DNA condensation potential cellular uptake efficiency. Various concentrations non-ionic ionic stabilizers to design formula colloidally stable cationic nanoemulsion. The nanoemulsion comprised 5% Capmul MCM, 0.5%...

Aims: In the present investigation, feasibility of fabricating novel self-assembled cationic nanocarriers (LeciPlex) containing cetyltrimethylammonium bromide (CTAB) or didodecyldimethylammonium (DDAB) and soybean lecithin using pharmaceutically acceptable biocompatible solvents such as 2-Pyrrolidone (Soluphor P®) diethyleneglycol monoethyl ether (Transcutol®) was established. Materials & Methods: The interaction between DDAB/CTAB in confirmed by differential scanning calorimetry vitro...

Persistent infections with high-risk (HR) human papillomaviruses such as HPV 16, 18, 31, 33 and 45 have been identified the major aetiological factor for cervical cancer. The clinical outcome of disease is often determined by viral factors load, physical status oncogene expression. aim present study was to evaluate impact on in HPV16 positive, locally advanced cancer cases.One hundred thirty two pretreatment tumour biopsies were selected from patients undergoing radiotherapy alone (n=63) or...

Abstract Background The prevalence of human papillomavirus (HPV) infection in India is high. HPV known to cause cervical cancer and has also been implicated the pathogenesis retinoblastoma (RB), a common intraocular malignant tumor childhood which can be familial or sporadic. Despite high incidence RB India, its form rare. Hence this study was undertaken investigate whether high‐risk types 16 18 are involved development RB. Methods Formalin fixed paraffin embedded tissues (n = 76) including...

In order to elucidate a combination of genetic alterations that drive tobacco carcinogenesis we have explored unique model system and analytical method for an unbiased qualitative quantitative assessment gene-gene gene-environment interactions. The objective this case control study was assess predisposition in biologically enriched clinical related cancers (TRC), occurring as Multiple Primary Neoplasms (MPN).Genotyping 21 candidate Single Nucleotide Polymorphisms (SNP) from major metabolic...

Enhancing factor (EF), a growth regulatory molecule, isolated from mouse small intestines, has been well characterized in this laboratory. It increases the binding of epidermal unique manner via its own receptor. In first 20 N‐terminal amino acids sequenced, EF showed 50% homology to human Group II phospholipase A2 (PLA2). Here we propose that is yet another, unidentified isoform ofPLA2 which regulates cell proliferation modulation EGF binding. To our knowledge, report implicating...

Safety, efficacy and enhanced transgene expression are the primary concerns while using any vector for gene therapy. One of widely used vectors in clinical trials is adenovirus which provides a safe way to deliver therapeutic gene. However, has poor transduction efficiency vivo since most tumor cells express low coxsackie receptors. Similarly remains low, possibly because chromatization adenoviral genome upon infection eukaryotic cells, an effect mediated by histone deacetylases (HDACs)....