A5066088670- TGF-β signaling in diseases
- Retinoids in leukemia and cellular processes
- Bone Metabolism and Diseases
- Developmental Biology and Gene Regulation
- Estrogen and related hormone effects
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- MicroRNA in disease regulation
- RNA modifications and cancer
- Congenital heart defects research
- Genomics and Rare Diseases
- Kruppel-like factors research
- Parathyroid Disorders and Treatments
- Cancer-related gene regulation
- Bone Tissue Engineering Materials
- Molecular Biology Techniques and Applications
- Pluripotent Stem Cells Research
- Chemistry and Stereochemistry Studies
- Cancer-related Molecular Pathways
- Muscle Physiology and Disorders
- Animal Genetics and Reproduction
- Heterotopic Ossification and Related Conditions
- Cystic Fibrosis Research Advances
- Epigenetics and DNA Methylation
- Toxoplasma gondii Research Studies
King's College London
2024
Campbell University
2023
Rutgers New Jersey Medical School
2007-2022
Rutgers, The State University of New Jersey
2009-2022
Children's Health Ireland at Crumlin
2010-2022
Octapharma (United States)
2022
Presbyterian College
2021
Cedar Crest College
2019-2020
Our Lady's Hospital
2010-2019
University College Dublin
2015-2018
We have previously isolated a cDNA clone for gene whose expression is reduced by retinoic acid (RA) treatment of F9 embryonal carcinoma cells. The nucleotide sequence indicated that this gene, Rex-1, encodes zinc-finger protein and thus may be transcriptional regulator. Rex-1 message level high in two lines embryonic stem cells (CCE D3) when D3 are induced to differentiate using four different growth conditions. As expected stem-cell-specific message, mRNA present the inner cell mass (ICM)...
ABSTRACT G-to-A hypermutation has been sporadically observed in human immunodeficiency virus type 1 (HIV-1) proviral sequences from patient peripheral blood mononuclear cells (PBMC) and cultures but not systematically evaluated. PCR primers matched to normal hypermutated were used conjunction with an agarose gel electrophoresis system incorporating AT-binding dye visualize, separate, clone, sequence the 297-bp HIV-1 protease gene amplified PBMC. Among 53 patients, including individuals...
We explore three possible pathways for the evolution of genomic imprinting. (1) Imprinting may be advantageous in itself when imprinted and unimprinted alleles a locus confer different phenotypes. If segment DNA is gametes one sex but not those other, it might lead to effects correlated with sexual dimorphism. More fundamentally, certain organisms, determination have evolved because When imprinting leads chromosome elimination or inactivation occurs some embryos others, two classes embryos,...
Bone morphogenetic proteins-2 and -4 (BMPs-2 -4) are transforming growth factor beta-related proteins that can induce bone formation in vivo. We observed the level of endogenous BMP-2 mRNA increased an average 11-fold on differentiation F9 embryonal carcinoma cells into parietal endoderm after treatment with retinoic acid (RA) cAMP, whereas message for closely related BMP-4 decreased 12-fold this treatment. Therefore, effects exogenous recombinant protein RA-induced were investigated....
The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of reduced at transcriptional level when are induced by addition retinoic acid (RA) to differentiate. We have isolated genomic DNA (Zfp-42), characterized gene's structure, mapped mouse chromosome 8. Promoter elements contributing regulation promoter been identified. A region required activity contains an octamer motif (ATTTGCAT) which a...
Bone morphogenetic protein 2 (BMP2) is an essential growth factor and morphogen, whose pattern level of expression profoundly influences development physiology. We present the novel finding that mycoplasma infection induces BMP2 RNA production in six cell lines diverse types (mesenchymal, epithelial, myeloid). Mycoplasma triggered mature secreted BEAS-2B cells (immortalized human bronchial epithelial cells), which normally do not express BMP2, further increased A549 (lung adenocarcinoma...
The coding regions of many metazoan genes are highly similar. For example, homologs to the key developmental factor bone morphogenetic protein (BMP) 2 have been cloned by sequence identity from arthropods, mollusks, cnidarians, and nematodes. Wide conservation sequences suggests that differential gene expression explains vast morphological differences between species. To test hypothesis regulatory mechanisms controlling this evolutionarily ancient critical conserved, we compared flanking...
Abstract A classic morphogen, bone morphogenetic protein 2 (BMP2) regulates the differentiation of pluripotent mesenchymal cells. High BMP2 levels promote osteogenesis or chondrogenesis and low adipogenesis. inhibits myogenesis. Thus, synthesis is tightly controlled. Several hundred nucleotides within 3′ untranslated regions genes are conserved from mammals to fishes indicating that region under stringent selective pressure. Our analyses indicate this controls by post‐transcriptional...
Bmp2, a highly conserved member of the transforming growth factor-β gene family, is crucial for normal development. Retinoic acid, combined with cAMP analogs, sharply induces Bmp2 mRNA during differentiation F9 embryonal carcinoma cells into parietal endoderm. acid (RA) also in chick limb buds. Since normalBmp2 expression may require an endogenous retinoid signal and aberrant cause some aspects RA-induced teratogenesis, we studied mechanism underlying induction Bmp2. Measurements Bmp2mRNA...
In situ hybridization to cellular RNA is used determine the localization of specific messages within complex cell populations and tissues. Tissues may either be embedded in paraffin sectioned on a microtome (see Hybridization Using Paraffin Sections Cells), or frozen cryostat Cryosections). contained specimens hybridized radiolabeled probe Synthesis (35)S-Labled Riboprobes Double-Stranded DNA Probes), which then detected using film autoradiography emulsion (Chapter 8).
We have examined the abundance and cell specificity of several mRNAs that are regulated during retinoic acid (RA)-induced differentiation F9 embryonal carcinoma cells to visceral endoderm. The experiments confirmed multistep nature this process by demonstrating expression ERA-1/Hox 1.6 message within 6 h after RA addition; messages specific for extracellular matrix proteins laminin B1 B2, collagen IV(alpha 1) between days 4 12; two endoderm markers, alpha-fetoprotein (AFP) H19, 8-15. In situ...
The bone morphogenetic protein (BMP)2 gene has been genetically linked to osteoporosis and osteoarthritis. We have shown that the 3'-untranslated regions (UTR) of BMP2 genes from mammals fishes are extraordinarily conserved. This indicates 3'-UTR is under stringent selective pressure. present evidence conserved region a strong posttranscriptional regulator expression. Polymorphisms in cis-regulatory elements proven influence susceptibility growing number diseases. A common single nucleotide...
The high genetic heterogeneity of HIV-1 in the Democratic Republic Congo (DRC) constitutes a real challenge for development vaccines to counter spread epidemic. It is important continue monitor epidemic by studying circulating strains and their impact on overall spread. As part ongoing effort study global distribution subtypes recombinant forms (CRFs), here we describe new phylogenetic clade analysis two full-length sequences (83CD003 90CD121E12) collected from individuals at 7-year interval...
Bone morphogenetic protein (BMP) orthologs from diverse species like flies and humans are functionally interchangeable play key roles in fundamental processes such as dorso-ventral axis formation metazoans. Because both transcriptional post-transcriptional mechanisms central modulating developmental levels, we have analyzed the 3′-untranslated region (3′UTR) of Bmp 2 gene. This 3′UTR is unusually long alternatively polyadenylated. Mouse, human, dog mRNAs 83–87% identical within this region....