A5074488964- Veterinary Oncology Research
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Plant Toxicity and Pharmacological Properties
- Microbial infections and disease research
- Viral Infections and Vectors
- Mycobacterium research and diagnosis
- Virology and Viral Diseases
- Drug-Induced Hepatotoxicity and Protection
- Microscopic Colitis
- Viral gastroenteritis research and epidemiology
- Infant Nutrition and Health
- Probiotics and Fermented Foods
- Animal Virus Infections Studies
- Neurological diseases and metabolism
- Animal health and immunology
- Diagnosis and treatment of tuberculosis
- Veterinary Medicine and Surgery
- Antimicrobial Peptides and Activities
- Trace Elements in Health
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Ruminant Nutrition and Digestive Physiology
- Gastrointestinal motility and disorders
- Molecular Biology Techniques and Applications
Texas A&M University
2017-2025
Mitchell Institute
2023
Universidade Federal de Minas Gerais
2021-2022
College Station Medical Center
2019
Universidade Federal de Santa Maria
2011-2017
University of Georgia
2014
Clínica Santa María
2014
Clinica Santa Maria
2014
Fecal microbiota transplantation (FMT) has been described useful as an adjunct treatment for chronic enteropathy in dogs. Different protocols can be used to prepare and store FMT preparations, however, the effect of these methods on microbial viability is unknown. We aimed (1) assess several core intestinal bacterial species by qPCR (2) Peptacetobacter (Clostridium) hiranonis through culture further characterize different preparations. Bacterial abundances were assessed feces from six...
Intestinal absorption of bile acids is mediated by the apical sodium-dependent acid transporter (ASBT). Fecal dysmetabolism has been reported in dogs with chronic inflammatory enteropathy (CIE).Characterization ASBT distribution along intestinal tract control and comparison to CIE.Twenty-four CIE 11 dogs.The mRNA protein expression were assessed using RNA situ hybridization immunohistochemistry, respectively. The concentrations fecal measured gas chromatography-mass spectrometry. microbiota...
Post-weaning enteropathies in swine caused by pathogenic E. coli, such as post-weaning diarrhea (PWD) or edema disease (ED), remain a significant problem for the industry. Reduction use of antibiotics over concerns antibiotic resistance and public health concerns, necessitate evaluation effective alternatives to prevent loss livestock and/or reductions growth performance. For this purpose, an appropriate piglet model coli enteropathy is required. In study, we attempted induce clinical signs...
Bile acids (BAs) are important signaling molecules in the gastrointestinal (GI) tract and associated with health disease humans animals. Intestinal bacteria transform BA through deconjugation, dehydroxylation, epimerization reactions, producing various isoforms, many of which have not been investigated companion animal diseases. We aimed to develop analytically validate a novel liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for quantification 30 BAs dog feces, simple...
The intestinal microbiota is believed to play a role in the pathogenesis of inflammatory bowel disease humans and chronic enteropathy (CIE) dogs. While most previous studies have described gut using sequencing methods, it fundamental assess spatial distribution bacteria for better understanding their relationship with host. colonic mucosa 22 dogs CIE 11 control was investigated fluorescence situ hybridization (FISH) universal eubacterial probe (EUB338) specific probes select bacterial...
Several drug screening campaigns identified Calpeptin as a candidate against SARS-CoV-2. Initially reported to target the viral main protease (Mpro), its moderate activity in Mpro inhibition assays hints at second target. Indeed, we show that is an extremely potent cysteine cathepsin inhibitor, finding additionally supported by X-ray crystallography. Cell infection proved Calpeptin's efficacy Treatment of SARS-CoV-2-infected Golden Syrian hamsters with sulfonated dose 1 mg/kg body weight...
Introduction Canine protein-losing enteropathy (PLE) is a syndrome characterized by gastrointestinal loss of proteins. While fecal microbiome and metabolome perturbations have been reported in dogs with chronic enteropathy, they not widely studied PLE. Therefore, the study aims were to investigate gut targeted metabolites inflammatory PLE (iPLE) evaluate whether treatment affects these changes at short-term follow-up. Methods Thirty-eight histopathological evidence inflammation 47 healthy...
Parrot bornaviruses (PaBVs) are the causative agents of proventricular dilatation disease, however key aspects its pathogenesis, such as route infection, viral spread and distribution, target cells remain unclear. Our study aimed to track lesion development at 5, 10, 20, 25, 35, 40, 60, 80, 95 114 dpi using histopathology, immunohistochemistry, RT-PCR. After intramuscular inoculation parrot bornavirus 2 (PaBV-2) in pectoral muscle cockatiels, this virus was first detected macrophages...
Abstract Background Differentiation of lymphocytic‐plasmacytic enteropathy (LPE) from small cell lymphoma (SCL) in cats can be challenging. Hypothesis/Objective Histology‐guided mass spectrometry (HGMS) is a suitable method for the differentiation LPE SCL cats. Animals Forty‐one with and 52 SCL. Methods This retrospective clinicopathologic study. Duodenal tissue samples 17 22 were subjected to HGMS, acquired data used develop linear discriminate analysis (LDA) machine learning algorithm. The...
Foram revisados casos de defeitos congênitos (DCs) diagnosticados em bovinos no Laboratório Patologia da Universidade Federal Santa Maria 1964-2010. Durante o período estudado, foram examinados materiais provenientes necropsia 7.132 e encontrados 31 bezerros (0,4%) com DCs, os quais classificados 34 tipos alocados nos sistemas orgânicos primariamente afetados. Os DCs ocorriam isoladamente (19 [61,3%]) ou afetavam múltiplos sítios anatômicos (15 [28,7%]) frequência semelhante ambos sexos....
As doenças de chinchilas foram estudadas através da avaliação laudos necropsia entre janeiro 1997 e dezembro 2011. Em 202 necropsiadas, 189 (93,5%) tiveram o diagnóstico determinado, 13 (6,5%) inconclusivo, por ausência lesões ou autólise acentuada. Dentre as computadas, 162 eram fêmeas (80%), 37 machos (18%), em quatro (2%) sexo não foi anotado. tinham um dia a 12 anos idade. agrupadas nas seguintes categorias: inflamatórias, causadas intoxicações, agentes físicos, metabólicas,...
Neurotropism is a striking characteristic of bornaviruses, including parrot bornavirus 2 (PaBV-2). Our study evaluated the distribution inflammatory foci and viral nucleoprotein (N) antigen in brain spinal cord 27 cockatiels ( Nymphicus hollandicus) following experimental infection with PaBV-2 by injection into pectoral muscle. Tissue samples were taken at 12 timepoints between 5 114 days post-inoculation (dpi). Each group had approximately 3 per usually 1 negative control. Immunolabeling...
This paper describes an outbreak of chronic Senecio spp. poisoning in grazing sheep Rio Grande do Sul, Brazil, causing the death 10 out 860 adult sheep. Eight sick ewes were euthanized and necropsied. Cattle from this farm also affected. Clinical signs included progressive weight loss, apathy photosensitization. Four seven tested had increased gamma-glutamyl transferase serum activity two them presented elevation alkaline phosphatase. At necropsy, three eight slightly irregular toughened...
Senecio spp. poisoning is the main cause of cattle mortality in central region Rio Grande do Sul. This paper reports an outbreak seneciosis with high prevalence photosensitization, where 83 out 162 cows (51.3%) presented this clinical sign. The occurred September 2013, affecting adult that were held a 205 hectare-pasture from April to October 2013 abundant brasiliensis infestation. Main signs weight loss, excessive lacrimation or mucopurulent ocular discharge, nasal serous ventral diphteric...
Takeda-G-protein-receptor-5 (TGR5) is a receptor for bile acids and its expression has been described in variety of tissues species. Characterization TGR5 distribution function investigated drug discovery the treatment metabolic diseases humans. Because dogs are one species used biomedical research share some similarities with human gastrointestinal diseases, objective this study was to characterize canine This characterizes tract, liver, gallbladder, pancreas 8 dogs. The antigen mRNA using...
Abstract Fixation is the first step towards preservation of tissues and can impact downstream histological applications. Historically, formalin has been fixative choice in both research clinical settings due to cost, accessibility, broad applicability. Here, we describe a method for collection porcine colon, compare usage Carnoy’s solution (CS) 10% neutral buffered (NBF) tissue fixation. Consecutive colon samples were collected from 24 four-wk-old piglets fixed CS 45 min or NBF h. We...
Association between ovine β-globin polymorphisms and resistance against haemonchosis was described related to the mechanism of high oxygen affinity βA ➔ βC switch during anaemia, but there are no studies regarding involved local host responses. Phenotypic parameters responses were evaluated in sheep from two haplotypes naturally infected with Haemonchus contortus. Morada Nova lambs monitored at 63, 84 105 days age for faecal egg counts packed cell volume (PCV) under natural infection H. At...
RESUMO: Intoxicações por plantas do gênero Senecio representam uma importante causa de morte em animais produção, sendo brasiliensis a espécie mais envolvida nos casos intoxicação espontânea. Nesse trabalho, são descritos os aspectos epidemiológicos, clínicos e anatomopatológicos um surto natural equinos. Dois equinos, total dezoito, foram afetados. Os ocorreram propriedade rural no município São Martinho da Serra, Rio Grande Sul, Brasil. quantidade exemplares estágio brotação foi encontrada...
ABSTRACT: This study stems from the findings during gross and histopathological exam of 3,338 cattle brains as part bovine spongiform encephalopathy (BSE) active surveillance program Brazilian Ministry Agriculture, Livestock, Supply 2001 to 2005. The work was carried out in Veterinary Pathology Laboratory Federal University Santa Maria which at time (2001-2007) national reference laboratory for diagnosis BSE other transmissible encephalopathies. Both aspects are described. Several were...
A 1.5-year-old American quarter horse gelding (case 1) and an 11-month-old filly 2) were presented for acute onset pelvic lameness lethargy. Case 1 had nasal discharge, while case 2 developed rapid muscle atrophy. Both horses elevated serum creatine kinase activity. The showed similar polyphasic histiocytic lymphoplasmacytic myositis with necrosis, mineralization, regeneration. Additionally, Streptococcus equi subsp. e qui-induced suppurative retropharyngeal lymphadenitis renal purpura...