A5066396637- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- Animal Virus Infections Studies
- Virology and Viral Diseases
- COVID-19 epidemiological studies
- Infection Control and Ventilation
- Respiratory viral infections research
- COVID-19 and healthcare impacts
- Plant Virus Research Studies
- SARS-CoV-2 detection and testing
- Rabies epidemiology and control
- Microbial infections and disease research
- Molecular Biology Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Vaccine Coverage and Hesitancy
- interferon and immune responses
- Identification and Quantification in Food
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Romani and Gypsy Studies
- COVID-19 and Mental Health
- Virus-based gene therapy research
- Immune responses and vaccinations
- Aquaculture disease management and microbiota
Hamburg Port Consulting (Germany)
2022
Northern Norway Regional Health Authority
2021
Institut für Hygiene und Umwelt
2007-2013
University of Bonn
2008-2011
Katholisches Kinderkrankenhaus Wilhelmstift
2011
Bernhard Nocht Institute for Tropical Medicine
2008
Universidade Federal da Bahia
2008
Influenza A viruses are a threat to humans due their ability cross species barriers, as illustrated by the 2009 H1N1v pandemic and sporadic H5N1 transmissions. Interspecies transmission requires adaptation of viral polymerase importin-α, cellular protein that mediates transport into nucleus where transcription replication genome takes place. In this study, we analysed replication, host specificity pathogenicity avian mammalian influenza viruses, in importin-α-silenced cells...
This article describes multiple transmissions of rabies via transplanted solid organ from a single infected donor. The empirical Milwaukee treatment regimen was used in the recipients.Symptomatic patients were treated by deep sedation (ketamine, midazolam, and phenobarbital), ribavirin, interferon, active passive vaccination. Viral loads antibodies continuously monitored.Recipients both cornea liver transplants developed no symptoms. recipient transplant had been vaccinated approximately 20...
Parechovirus epidemiology and disease association are not fully understood. Real-time reverse transcriptase PCR (RT-PCR) for all human parechoviruses (HPeV) was applied on stool samples from two groups of patients. Both contained patients with acute enteritis age groups, seen during one full year. Patients norovirus, adenovirus, enterovirus, astrovirus, or rotavirus infections were excluded. In 118 outbreak hospital settings, no HPeV detected. a prospective study group 499 nonhospitalized...
ABSTRACT A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection 15.7 copies/reaction. clinical in patients with severe acute respiratory syndrome 100 copies/sample. In 39 children suffering from 229E, NL63, OC43, or HKU1, sensitivity equal to that individual real-time RT-PCRs.
Abstract Cardioviruses cause serious disease, mainly in rodents, including diabetes, myocarditis, encephalomyelitis, and multiple sclerosis–like disseminated encephalomyelitis. Recently, a human virus isolate obtained 25 years ago, termed Saffold virus, was sequenced classified as cardiovirus. We conducted systematic molecular screening for Saffold-like viruses 844 fecal samples from patients with gastroenteritis Germany Brazil, across all age groups. Six cardioviruses were identified <6 of...
Influenza A(H1N1)v virus was first identified in April 2009. A novel real-time RT-PCR for influenza set up ad hoc and validated following industry-standard criteria. The lower limit of detection the assay 384 copies viral RNA per ml transport medium (95% confidence interval: 273-876 copies/ml). Specificity 100% as assessed on a panel reference samples including seasonal human H1N1 H3N2, highly pathogenic avian H5N1 porcine H1N1, H1N2 H3N2 samples. matrix gene recommended 2007 by World Health...
In developed countries, acute gastroenteritis (AGE) is a major source of morbidity. However, only few studies have estimated its incidence and the associated medical burden. This population-based study determined community-acquired AGE patients seeking care relative role various pathogens. Stool samples from with presenting to general practitioner (GP), pediatrician, or specialist in internal medicine for that reason were screened bacterial viral enteropathogens. A control group was...
Abstract A new strain of measles virus, D4-Hamburg, was imported from London to Hamburg in December 2008 and subsequently spread Bulgaria, where an outbreak >24,300 cases observed. We analyzed the virus demonstrate importance addressing hard-to-reach communities within World Health Organization European Region regarding access medical care vaccination campaigns. The D4-Hamburg appeared during 2009–2011 Poland, Ireland, Northern Austria, Greece, Romania, Turkey, Macedonia, Serbia,...
There is uncertainty about the viral loads of infectious individuals required to transmit COVID-19 via aerosol. In addition, there a lack both quantification influencing parameters on airborne transmission and simple-to-use models for assessing risk infection in practice, which furthermore quantify influence non-medical preventive measures. this study, dose-response model was adopted analyze 25 documented outbreaks at rates 4-100%. We show that only possible if load higher than 10
The role of schools as a source infection and driver in the coronavirus-pandemic has been controversial is still not completely clarified. To prevent harm disadvantages for children adolescents, but also adults, detailed data on school outbreaks needed, especially when talking about open employing evidence-based safety concepts. Here, we investigated first significant COVID-19 outbreak Hamburg, Germany, after re-opening 2020. Using clinical, laboratory, contact spatial measures...
This study identified the complete genomic sequence of four type 2 and 3 human Saffold-like cardioviruses (SLCVs) isolated in Germany Brazil. The secondary structures SLCV internal ribosome entry sites (IRESs) were deduced based on RNA base-pairing conservation co-variation, using an established Theiler's murine encephalomyelitis virus (TMEV) IRES structure as a reference. was highly similar to that TMEV, but motifs critical TMEV for binding polypyrimidine tract-binding protein (PTB)...
Abstract Background Enteritis is caused by a spectrum of viruses that most likely not fully characterised. When testing stool samples cell culture, virus isolates are sometimes obtained which cannot be typed current methods. In this study we used VIDISCA, identification method has yet been widely applied, on such an untyped isolate. Results We found human parechovirus (HPeV) type 1 (strain designation: BNI-788st). Because genomes contemporary HPeV1 were available, determined its complete...
Definite and rapid diagnosis of rabies is required for individual case management as well public health. For the first time, a direct comparison virus isolation with quantitative real-time reverse transcription (RT)-PCR on human samples was conducted. RT-PCR found to be more sensitive than isolation.
Aichi virus (AiV), a member of the genus Kobuvirus in family Picornaviridae, causes gastroenteritis humans. It was noted that AiV differs from other picornaviruses its unusually high C content and very degree genome-ordered RNA secondary structures. However, genetic variability mutational restrictions on full-genome scale have not been studied. In addition to available five complete genomes, we determined here another coding sequences sampled Germany, 2004. Distinctive features included low...
Abstract Currently, airborne transmission is seen as the most important path for SARS-CoV-2. In this investigation, a classic dose-response model used on one hand to find out retrospectively probable viral load of infectious source patient at time in 25 documented outbreaks. We showed that an infection due distance from person was probably only possible outbreaks examined, with attack rates 4-100%, if had been higher than 1E+08 copies/ml. This demonstrates estimated swab might overestimate...
Abstract Cardioviruses cause myocarditis and encephalomyelitis in rodents; human cardioviruses have not been ascribed to any disease. We screened 6,854 cerebrospinal fluid 10 myocardium specimens from children adults. A genotype 2 cardiovirus was detected a child who died of sudden infant death syndrome, untypeable were with meningitis.
From December 2008 to June 2009 a measles outbreak occurred in the Federal State of Hamburg, Germany. The affected 216 persons and was caused by new strain termed D4-Hamburg which led consecutive outbreaks between 2011 at least 12 European countries. Here, we describe epidemiological characteristics evaluate control measures taken Hamburg. In one seven boroughs Hamburg local Roma community comprised more than 50% notified cases.We compared stratified analysis age distribution these cases...
Previous reports of 16S rDNA sequencing members the family Halomonadaceae Franzmann et al. 1989 include two different sequences that were both attributed to type strain Halomonas salina (Valderrama 1991) Dobson and 1996 (basonym Deleya Valderrama 1991). The are sufficiently for them belong species within genus Halomonas. In order determine which corresponded salina, designated strains this obtained from ATCC DSMZ. It was possible show only one previous DSMZ 5928T 49509T.
Background: The role of schools as a source infection and driver in the coronavirus-pandemic has been controversial is still not completely clarified. To prevent harm disadvantages for children adolescents, but also adults, detailed data on school outbreaks needed, especially when talking about open employing evidence-based safety concepts. Here, we investigated first significant COVID-19 outbreak Hamburg, Germany, after re-opening 2020. Methods: Using clinical, laboratory, contact spatial...