A5037202012- Herpesvirus Infections and Treatments
- HIV Research and Treatment
- Cytomegalovirus and herpesvirus research
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Plant Virus Research Studies
- Immune Cell Function and Interaction
- Viral Infections and Vectors
- Toxin Mechanisms and Immunotoxins
- Poxvirus research and outbreaks
- Computational Drug Discovery Methods
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Viral-associated cancers and disorders
- Insect symbiosis and bacterial influences
- Microbial Metabolic Engineering and Bioproduction
- Insect Resistance and Genetics
- Microbial metabolism and enzyme function
- Metabolism and Genetic Disorders
- Transgenic Plants and Applications
- Insect and Arachnid Ecology and Behavior
- Natural Compound Pharmacology Studies
- Antimicrobial Peptides and Activities
- Virology and Viral Diseases
Bangladesh Livestock Research Institute
2024-2025
ICMR-National Institute of Virology
2023-2024
University of Edinburgh
1987-2005
We have isolated a maedi-visna-like virus from the peripheral blood mononuclear cells of British sheep displaying symptoms arthritis and pneumonia. After brief passage in fibroblasts this (designated EV1) was used to infect choroid plexus cells. cDNA clones were prepared these sequenced. Gaps between non-overlapping filled using gene amplification by polymerase chain reaction. The genome structure is similar that described for visna strain 1514, differs SA-OMVV not having W reading frame....
Metagenomic analysis of Aedes aegypti and Ae. albopictus mosquitoes from diverse geographical regions India revealed the presence several insect viruses human interest. Most abundant reads found in were Phasi Charoen-like virus (PCLV), Choristoneura fumiferana granulovirus (CfGV), Cell fusing agent (CFAV), Wenzhou sobemo-like 4 (WSLV4), whereas WSLV4 CfGV constituted highest percentage viromes. Other that low included Hubei mosquito 2 (HMV2), Porcine astrovirus (PAstV4), Wild Boar (WBAstV)....
ABSTRACT Lumpy skin disease (LSD) in cattle is managed through live-attenuated vaccines. Bangladesh LSD vaccine was developed from virus isolate LSD-29 by passaging 60 times cell culture. Here, we report the complete genome sequence of strain.
Dimethyl sulfoxide (DMSO) is frequently used at a concentration of up to 95% in the formulation antiherpetic agents because its properties as skin penetration enhancer. Here, we have analyzed effect DMSO on several parameters Herpes Simplex Virus replication. Productive infection levels HSV-1 were determined by plaque assay or reporter gene activity, and DNA replication was estimated PCR. Transcript evaluated with HSV-specific micro-arrays. blocks productive vitro different cell types 50%...
Reactive changes occurring within lymph nodes draining the subcutaneous site of acute infection with maedi-visna virus (MVV) were studied, and appearance infected cells correlated immune response. Cells detected in node by cocultivation from day 4 postinfection (p.i.), maximum numbers being seen between days 7 14, but even then rare, a frequency 23 50% tissue culture infective doses (TCID50) 10(6) cells. At later times, still detected, their fell to 1 2 TCID50 per Virus-specific CD8+...
The LPD1 gene of S. cerevisiae, which encodes lipoamide dehydrogenase (EC 1.8.1.4), has been cloned and characterized. is present as a single copy in the yeast genome transcribed to give polyadenylated mRNA species approximately 2·0 kb. synthesis subject carbon catabolite repression since both level transcript accumulation subunit polypeptide were greatly reduced wild-type cells grown on glucose compared those variety non-fermentable sources. Strains defective but transformed with high...
ABSTRACT Lumpy skin disease (LSD) is an economically devasting and vector-borne transboundary of cattle caused by lumpy virus (LSDV). Here, we report the complete genome sequence outbreak isolate LSDV from Bangladesh. Bangladesh LSD-29 was detected in nodule samples LSD-infected bovine 2021.
Microarray technology provides an opportunity to monitor multiple parameters simultaneously. High-throughput applications such as blood donation screening could greatly benefit from performing various tests on a single testing platform. Blood grouping represents one part of the complementing for blood-borne pathogens. group serology traditionally exploited agglutination detection method. In this investigation, we have adapted reactions solid-phase microarray substrate in non-agglutination...
DNA extraction is a routine step in many insect molecular studies and methods need to be evaluated for their efficiency, cost, side effects, such as degradation. Individuals of six spotted ladybird beetle, Cheilomenes sexmaculata (F.) were subjected by the CTAB method PureLink® Kit. The compared terms quantity quality, cost materials, time consumed. resulted higher yield (ng DNA) at much lower price less degradation, revealed on agarose gels. Kit was most time-efficient but costliest,...
During of COVID-19 pandemic, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has continuously evolved, resulting in the emergence several new variants concerns (VOCs) with numerous mutations. These VOCs dominate various regions due to increased transmissibility and antibody evasion, potentially reducing vaccine effectiveness. Nonetheless, it remains uncertain whether recent SARS-CoV-2 have ability circumvent T cell immunity elicited by either vaccination or natural infection....
Metagenomic analysis of Aedes aegypti and Ae. albopictus mosquitoes from diverse geographical regions India revealed presence several insect viruses human interest. Most abundant reads found in were Phasi-Charoen like virus (PCLV), Choristoneura fumiferana granulovirus (CfGV), Cell fusing agent (CFAV) Wenzhou sobemo-like 4 (WSLV4), whereas WSLV4 CfGV constituted the highest percentage viromes. Other which low included Hubei mosquito 2, Porcine astrovirus Wild Boar astrovirus. PCLV CFAV, to...
Conference Abstract| October 01 2000 Green fluorescent protein expressing recombinant virus: a useful tool for in vivo study of natural gammaherpesvirus infection S. Selvarajah; Selvarajah 1Laboratory Clinical and Molecular Virology, Summerhall, University Edinburgh, EH9 1QH Search other works by this author on: This Site PubMed Google Scholar B. M. Dutia; Dutia D. J. Roy; Roy P. Stewart; Stewart A. Nash Biochem Soc Trans (2000) 28 (5): A213. https://doi.org/10.1042/bst028a213b Views Icon...