A5009780471- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Protein Kinase Regulation and GTPase Signaling
- Nerve injury and regeneration
- Neurotransmitter Receptor Influence on Behavior
- Periodontal Regeneration and Treatments
- Cell death mechanisms and regulation
- Neurogenesis and neuroplasticity mechanisms
- Pancreatic function and diabetes
- GABA and Rice Research
- Electroconvulsive Therapy Studies
- Treatment of Major Depression
- Advanced biosensing and bioanalysis techniques
- Pharmacological Receptor Mechanisms and Effects
- Lipid metabolism and biosynthesis
- Extracellular vesicles in disease
- Fungal and yeast genetics research
- Neurogenetic and Muscular Disorders Research
- Single-cell and spatial transcriptomics
- Ubiquitin and proteasome pathways
- Receptor Mechanisms and Signaling
- Mesenchymal stem cell research
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Retinoids in leukemia and cellular processes
University of the Basque Country
2012-2024
Worcester Polytechnic Institute
2015-2024
Platelet-Rich Plasma (PRP) is enriched in molecular messengers with restorative effects on altered tissue environments. Upon activation, platelets release a plethora of growth factors and cytokines, either free form or encapsulated exosomes, which have been proven to promote repair regeneration. Translational research the potential exosomes as safe nanosystem for therapeutic cargo delivery requires standardizing exosome isolation methods along their morphological characterization. With this...
Abstract The Gα q /phospholipase C-β (PLCβ) signaling system mediates calcium responses to a variety of hormones and neurotransmitters. Recent studies suggest that PLCβ1 expression plays role in the differentiation two types cultured neuronal cells (PC12 SK-N-SH) through mechanism independent . Here, we show that, similar observed PC12 SK-N-SH cells, increases when human NT2 are induced differentiate either cytosine-β-D-arabinofuranoside or retinoic acid. Preventing this increase, abolishes...
Platelet-rich plasma (PRP) is a biologic therapy that promotes healing responses across multiple medical fields, including the central nervous system (CNS). The efficacy of this depends on several factors such as donor's health status and age. This work aims to prove effect PRP cellular models CNS, considering differences between from young elderly donors. Two different pools were prepared donors 65‒85 20‒25 years old. molecular composition both PRPs analyzed. Subsequently, response was...
The transfection of human NTera2/D1 teratocarcinoma-derived cell line (or NT2 cells) represents a promising strategy for the delivery exogenous proteins or biological agents into central nervous system (CNS). development suitable nonviral vectors with high efficiencies requires profound knowledge whole process. In this work, we elaborated and characterized in terms size zeta potential three different vectors: lipoplexes (144 nm; −29.13 mV), nioplexes (142.5 +35.4 polyplexes (294.8 +15.1 mV)....
In this report, we describe the kinetics characteristics of diacylglycerol lipase-α (DGLα) located at nuclear matrix nuclei derived from adult cortical neurons. Thus, using high-resolution fluorescence microscopy, classical biochemical subcellular fractionation, and Western blot techniques, demonstrate that DGLα enzyme is in neuronal nuclei. Furthermore, by quantifying 2-arachidonoylglycerol (2-AG) level liquid chromatography mass spectrometry when 1-stearoyl-2-arachidonoyl-sn-glycerol (SAG)...
In this report, we describe the localization of diacylglycerol lipase-α (DAGLα) in nuclei from adult cortical neurons, as assessed by double-immunofluorescence staining rat brain sections and purified intact western blot analysis subnuclear fractions. Double-labeling assays using anti-DAGLα antibody NeuN combined with Hoechst showed that only neuronal origin were DAGLα positive. At high resolution, DAGLα-signal displayed a punctate pattern nuclear subdomains poor Hoechst's chromatin lamin B1...
The human NTERA2/D1 (NT2) cells generate postmitotic neurons (NT2N cells) upon retinoic acid (RA) treatment and are functionally integrated in the host tissue following grafting into rodent brain, thus representing a promising source for neuronal replacement therapy. Yet major limitations of this model lengthy differentiation procedure its low efficiency, although recent studies suggest that process can be shortened to less than 1 week using nucleoside analogues. To explore whether...
Specific and selective anti-CB1 antibodies are among the most powerful research tools to unravel complex biological processes mediated by CB1 receptor in both physiological pathological conditions. However, low performance of remains a major source inconsistency between results from different laboratories. Using variety techniques, including some commonly accepted ones for antibody specificity testing, we identified three five commercial against regions as best choice specific end-use...
NTERA2/D1 human teratocarcinoma progenitors induced to differentiate into postmitotic neurons by either long-term treatment with retinoic acid or short-term the nucleoside analog cytosine β-D-arabinofuranoside were subjected morphometric analysis and compared. Our data provide a methodological conceptual framework for future investigations aiming at distinguishing neuronal phenotypes on basis of analysis. Data presented here are related research concurrently published in "Highly Efficient...
Abstract Background Replacement of radioligand binding assays with antibody-antigen interaction-based approaches for quantitative analysis G protein-coupled receptor (GPCR) levels requires the use purified protein standards containing antigen. GPCRs in general and cannabinoid CB 1 particular show a progressive tendency to aggregate precipitate aqueous solution outside their biological context due low solubility that hydrophobic nature imprinted by seven transmembrane domains. This renders...
<title>Abstract</title> The Gα<sub>q</sub>/phospholipase C-β (PLCβ) signaling system mediates calcium responses to variety of hormones and neurotransmitters. Recent studies suggest that PLCβ1 expression plays a role in the differentiation two types cultured neuronal cells (PC12 SKNSH) through mechanism independent Gα<sub>q</sub>. Here, we show similar increase when human NT2 are induced differentiate either AraC or retinoic acid. Preventing this abolishes differentiation. Surprisingly,...
After peripheral nerve injury (PNI), prolonged denervation of the target muscle prevents adequate reinnervation even if is repaired. The aim this work to analyze effect intramuscular Platelet-Rich Plasma (PRP) in a denervated due PNI.Materials and.
The present study describes a detailed neuroanatomical distribution map of the cannabinoid type 1 (CB ) receptor, along with biochemical characterization expression and functional coupling to their cognate G i/o proteins in medial prefrontal cortex (mPCx) obese Zucker rats. CB receptor density was higher prelimbic (PL) infralimbic (IL) subregions mPCx rats relative lean littermates which associated percentage immunopositive excitatory presynaptic terminals PL IL. Also, receptors...
Beste Medikuntza-arloetan ez bezala, Psikiatrian gaixotasun psikiatrikoei buruzko hipotesi etiologikoak ebidentzia farmakologikoetan oinarritzen dira. Horrela, 1950eko hamarkadan depresioaren kontrako farmakoak garatzen hasi ziren, behin ikusita zer-nolako eraginak zituzten tuberkulosiaren farmako batzuek. Lehenengo antidepresiboek neuro-transmisio monoaminergikoa bultzatzen zutenez, sortu zen, eta depresioari ikerkuntzak serotoninaren, noradrenalinaren dopaminaren (monoaminen) gutxiagotzea...
Alboko esklerosi amiotrofikoa (AEA) gaixotasun neurodegeneratibo heterogeneo eta sendaezina da. Goiko beheko neurona motorren endekapena bizkarmuineko alboko kordoiaren esklerosia dira zigilu histopatologiko nagusiak. Adierazpen klinikoetan aldakortasun handia dagoen arren, ahultasun orokorra, faszikulazioak espasmoak gertatzen endekapenaren muskulu-atrofiaren ondorioz, baita portaera- kognizio-asaldurak ere garuneko kalte zabala dela eta. Mutazio genetikoek gaixotasunaren bi adierazpen...
Gaur egun, istripu zerebrobaskularra (IZB) mundu osoan bigarren heriotza-kausa eta ezgaitasunen hirugarren eragilea da. IZB, garuneko zirkulazioaren bat-bateko asaldura da, non, neuronen galera baten ondorioz, aldi baterako edo betirako garunaren eskualde jakin funtzioa eraldatzen den. Hortaz, praktika klinikoan, prebentzioan lan egiteaz gain, ezinbestekoa da funtzio neurologikoen galeraren oinarrian dagoen heriotzaren kontra zuzendutako tratamendu eraginkorrak izatea. Tamalez, gaur egun...