A5017030486- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Pancreatic function and diabetes
- GABA and Rice Research
- Neurotransmitter Receptor Influence on Behavior
- Neurogenesis and neuroplasticity mechanisms
- Apelin-related biomedical research
- Neuroscience and Neural Engineering
- Lipid metabolism and biosynthesis
- Glioma Diagnosis and Treatment
- Treatment of Major Depression
- Neuroscience of respiration and sleep
- Single-cell and spatial transcriptomics
- Pharmacology and Obesity Treatment
- Pharmacological Receptor Mechanisms and Effects
- Nerve injury and regeneration
- Electroconvulsive Therapy Studies
University of the Basque Country
2013-2023
In this report, we describe the kinetics characteristics of diacylglycerol lipase-α (DGLα) located at nuclear matrix nuclei derived from adult cortical neurons. Thus, using high-resolution fluorescence microscopy, classical biochemical subcellular fractionation, and Western blot techniques, demonstrate that DGLα enzyme is in neuronal nuclei. Furthermore, by quantifying 2-arachidonoylglycerol (2-AG) level liquid chromatography mass spectrometry when 1-stearoyl-2-arachidonoyl-sn-glycerol (SAG)...
In this report, we describe the localization of diacylglycerol lipase-α (DAGLα) in nuclei from adult cortical neurons, as assessed by double-immunofluorescence staining rat brain sections and purified intact western blot analysis subnuclear fractions. Double-labeling assays using anti-DAGLα antibody NeuN combined with Hoechst showed that only neuronal origin were DAGLα positive. At high resolution, DAGLα-signal displayed a punctate pattern nuclear subdomains poor Hoechst's chromatin lamin B1...
The human NTERA2/D1 (NT2) cells generate postmitotic neurons (NT2N cells) upon retinoic acid (RA) treatment and are functionally integrated in the host tissue following grafting into rodent brain, thus representing a promising source for neuronal replacement therapy. Yet major limitations of this model lengthy differentiation procedure its low efficiency, although recent studies suggest that process can be shortened to less than 1 week using nucleoside analogues. To explore whether...
Specific and selective anti-CB1 antibodies are among the most powerful research tools to unravel complex biological processes mediated by CB1 receptor in both physiological pathological conditions. However, low performance of remains a major source inconsistency between results from different laboratories. Using variety techniques, including some commonly accepted ones for antibody specificity testing, we identified three five commercial against regions as best choice specific end-use...
NTERA2/D1 human teratocarcinoma progenitors induced to differentiate into postmitotic neurons by either long-term treatment with retinoic acid or short-term the nucleoside analog cytosine β-D-arabinofuranoside were subjected morphometric analysis and compared. Our data provide a methodological conceptual framework for future investigations aiming at distinguishing neuronal phenotypes on basis of analysis. Data presented here are related research concurrently published in "Highly Efficient...
The present study describes a detailed neuroanatomical distribution map of the cannabinoid type 1 (CB ) receptor, along with biochemical characterization expression and functional coupling to their cognate G i/o proteins in medial prefrontal cortex (mPCx) obese Zucker rats. CB receptor density was higher prelimbic (PL) infralimbic (IL) subregions mPCx rats relative lean littermates which associated percentage immunopositive excitatory presynaptic terminals PL IL. Also, receptors...
Beste Medikuntza-arloetan ez bezala, Psikiatrian gaixotasun psikiatrikoei buruzko hipotesi etiologikoak ebidentzia farmakologikoetan oinarritzen dira. Horrela, 1950eko hamarkadan depresioaren kontrako farmakoak garatzen hasi ziren, behin ikusita zer-nolako eraginak zituzten tuberkulosiaren farmako batzuek. Lehenengo antidepresiboek neuro-transmisio monoaminergikoa bultzatzen zutenez, sortu zen, eta depresioari ikerkuntzak serotoninaren, noradrenalinaren dopaminaren (monoaminen) gutxiagotzea...