A5082237171- Neuroscience and Neuropharmacology Research
- Cannabis and Cannabinoid Research
- Sleep and Wakefulness Research
- Ion channel regulation and function
- Neurotransmitter Receptor Influence on Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion Channels and Receptors
- Forensic Toxicology and Drug Analysis
- Biochemical Analysis and Sensing Techniques
- Pain Mechanisms and Treatments
- Glycogen Storage Diseases and Myoclonus
- Biochemical effects in animals
- Cardiac electrophysiology and arrhythmias
- Neuroscience of respiration and sleep
- Hearing, Cochlea, Tinnitus, Genetics
- Basque language and culture studies
- Lipid Membrane Structure and Behavior
- Olfactory and Sensory Function Studies
- Spanish Linguistics and Language Studies
- Genetic Neurodegenerative Diseases
- Neurological and metabolic disorders
- Neural dynamics and brain function
- Interpreting and Communication in Healthcare
- Memory and Neural Mechanisms
- Cancer Diagnosis and Treatment
University of the Basque Country
2013-2024
Achucarro Basque Center for Neuroscience
2015-2022
Euskadiko Parke Teknologikoa
2015-2016
Cannabinoids have deleterious effects on prefrontal cortex (PFC)-mediated functions and multiple evidences link the endogenous cannabinoid (endocannabinoid) system, cannabis use schizophrenia, a disease in which PFC are altered. Nonetheless, molecular composition physiological of endocannabinoid system unknown.Here, using electron microscopy we found that key proteins involved signaling expressed layers v/vi mouse prelimbic area PFC: presynaptic CB1 receptors (CB1R) faced postsynaptic mGluR5...
The cannabinoid type 1 (CB1) receptor is widely distributed in the brain and peripheral organs where it regulates cellular functions metabolism. In brain, CB1 mainly localized on presynaptic axon terminals but also found mitochondria (mtCB1), respiration energy production. Likewise, muscle mitochondria, very little known about it. aim of this study was to further investigate detail distribution functional role mtCB1 three different striated muscles. Immunoelectron microscopy for used...
Abstract Astroglial type‐1 cannabinoid (CB 1 ) receptors are involved in synaptic transmission, plasticity and behavior by interfering with the so‐called tripartite synapse formed pre‐ post‐synaptic neuronal elements surrounding astrocyte processes. However, little is known concerning subcellular distribution of astroglial CB receptors. In particular, brain mostly localized at cells' plasmalemma, but recent evidence indicates their functional presence mitochondrial membranes. Whether present...
The endocannabinoid system is involved in multiple physiological functions including reward. Cannabinoids potently control the activity of midbrain dopamine cells, but contribution cortical projections this phenomenon unclear. We show that bed nucleus stria terminalis (BNST) efficient relays excitation to neurons ventral tegmental area (VTA). Anatomical and vivo electrophysiological evidence demonstrate excitatory arising exclusively from infralimbic cortex converge on BNST neurons, which...
The distribution and roles of the cannabinoid CB2 receptor in CNS are still a matter debate. Recent data suggest that, addition to its presence microglial cells, may be also expressed at low levels, yet biologically relevant, other cell types such as neurons. It is accepted that expression receptors under physiological conditions significantly elevated chronic neuroinflammatory states associated with neurodegenerative diseases Alzheimer's disease. By using novel mouse model (CB2EGFP/f/f), we...
Background The bed nucleus of the stria terminalis (BNST) is involved in behaviors related to natural reward, drug addiction and stress. In spite emerging role endogenous cannabinoid (eCB) system these behaviors, little known about anatomy function this anterolateral BNST (alBNST). aim study was provide a detailed morphological characterization localization 1 (CB1) receptor necessary step toward better understanding physiological roles eCB region brain. Methodology/Principal Findings We have...
ABSTRACT Type 1 cannabinoid (CB ) receptors are widely distributed in the brain. Their physiological roles depend on their distribution pattern, which differs remarkably among cell types. Hence, subcellular compartments with little but functionally relevant CB can be overlooked, fostering an incomplete mapping. To overcome this, knockin mice cell‐type–specific rescue of have emerged as excellent tools for investigating receptors’ localization and sufficient functional role no bias. However,...
The endocannabinoid system modulates synaptic plasticity in the hippocampus, but a link between long-term and type 1 cannabinoid (CB1) receptor at medial perforant path (MPP) synapses remains elusive. Here, immuno-electron microscopy adult mice showed that ∼26% of excitatory terminals middle 1/3 dentate molecular layer (DML) contained CB1 receptors, field postsynaptic potentials evoked by MPP stimulation were inhibited activation. In addition, 10 Hz for min triggered receptor-dependent...
Several metabotropic glutamate receptor (mGluR) subtypes have been identified in the cerebellar cortex that are targeted to different compartments cells. In this study, preembedding immunocytochemical methods for electron microscopy were used investigate subcellular distribution of mGluR1b splice variant rat cortex. Dendritic spines Purkinje cells receiving parallel fiber synaptic terminals immunoreactive mGluR1b. With a immunogold method, ~25% immunolabeling was observed perisynaptically...
Neonatal hypoxia-ischemia (HI) is a risk factor for myelination disturbances, key cerebral palsy. Cannabidiol (CBD) protects neurons and glial cells after HI insult in newborn animals. We hereby aimed to study CBD's effects on long-lasting HI-induced deficits rats. Thus, P7 Wistar rats received s.c. vehicle (HV) or cannabidiol (HC) brain damage (left carotid artery electrocoagulation plus 10% O2 112 min). Controls were non-HI pups. At P37, neurobehavioral tests performed immunohistochemistry...
Studies indicate that metabotropic glutamate receptors (mGluRs) may play a role in spinal sensory transmission. We examined the cellular and subcellular distribution of mGluR subtype 4a tissue by means specific antiserum immunocytochemical techniques for light electron microscopy. A dense plexus mGluR4a-immunoreactive elements was seen dorsal horn, with an apparent accumulation lamina II. The immunostaining composed sparse immunoreactive fibres punctate elements. No perikaryal staining seen....
Background Type-1 cannabinoid receptors (CB1R) are enriched in the hypothalamus, particularly ventromedial hypothalamic nucleus (VMH) that participates homeostatic and behavioral functions including food intake. Although CB1R activation modulates excitatory inhibitory synaptic transmission brain, contribution to molecular architecture of terminals VMH is not known. Therefore, aim this study was investigate precise subcellular distribution better understand modulation exerted by...
Abstract Cannabinoid type‐1 (CB 1 ) receptors are widely distributed in the brain and play important roles astrocyte function modulation of neuronal synaptic transmission plasticity. However, it is currently unknown how CB receptor expression astrocytes affected by long‐term exposure to stressors. Here we examined ethanol (EtOH)‐exposed adolescent mice determine its effect on localization density adult brain. 4–8‐week‐old male were exposed 20 percent EtOH over a period 4 weeks, was after...
A preembedding immunocytochemical method for light microscopy was used to study the postnatal development of expression group III metabotropic glutamate receptor mGluR4a in medial nucleus trapezoid body (MNTB) rat. Immunoreactivity localized axonal endings wrapping principal globular neurons MNTB, known as calyces Held. The percentage Held immunoreactive increased progressively from day 3 (PND3), showing highest density labeled by PND9. From this age on, a gradual reduction number...
Abstract Binge drinking (BD) is a common pattern of ethanol (EtOH) consumption by adolescents. The brain effects the acute EtOH exposure are well‐studied; however, long‐lasting cognitive and neurobehavioral consequences BD during adolescence only beginning to be elucidated. Environmental enrichment (EE) has long been known for its benefits on may serve as potential supportive therapy following exposure. In this study, we hypothesized that EE have deficits associated with consumption....
The transient receptor potential vanilloid 1 (TRPV1) is a non-selective ligand-gated cation channel involved in synaptic transmission, plasticity, and brain pathology. In the hippocampal dentate gyrus, TRPV1 localizes to dendritic spines dendrites postsynaptic excitatory synapses molecular layer (ML). At these same synapses, cannabinoid CB (CB R) activated by exogenous endogenous cannabinoids presynaptic terminals. Hence, as both receptors are anandamide, co-localize, mediate long-term...