A5081520776- Immune Response and Inflammation
- Sepsis Diagnosis and Treatment
- Intensive Care Unit Cognitive Disorders
- Immune cells in cancer
- Burn Injury Management and Outcomes
- Trauma and Emergency Care Studies
- Gallbladder and Bile Duct Disorders
- Cardiac, Anesthesia and Surgical Outcomes
- Vitamin C and Antioxidants Research
- Inflammation biomarkers and pathways
- Wound Healing and Treatments
- Heart Rate Variability and Autonomic Control
- Surgical Simulation and Training
- Neonatal Respiratory Health Research
- Immune responses and vaccinations
- S100 Proteins and Annexins
- Renal function and acid-base balance
- Uterine Myomas and Treatments
- T-cell and B-cell Immunology
- Injury Epidemiology and Prevention
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Frailty in Older Adults
- Stress Responses and Cortisol
- Electrolyte and hormonal disorders
- Surgical Sutures and Adhesives
Florida College
2022-2025
University of Florida
2020-2025
Gainesville Obstetrics & Gynecology
2023
Foundation for the National Institutes of Health
2022
National Institute of General Medical Sciences
2022
Alabama College of Osteopathic Medicine
2020
University of Florida Health Science Center
2020
University of Florida Health
2020
The University of Texas Southwestern Medical Center
2018-2019
Instituto Nacional de Cancerología
2018
Sepsis is a state of systemic immune dysregulation and organ failure that frequently associated with severe brain disability. Epidemiological studies have indicated younger females better prognosis clinical outcomes relative to males, though the sex-dependent response sepsis during post-sepsis recovery remains largely uncharacterized. Using modified polymicrobial intra-abdominal murine model surgical sepsis, we characterized acute effects on peripheral inflammation, inflammation functional...
In Brief Export A review of UF SCIRC's research efforts characterizing CCI, PICS biomarkers, effect site infection, acute kidney injury, effects on older patients, dysfunctional high-density lipoproteins, sarcopenia/cachexia, emergency myelopoiesis, and dysregulated erythropoiesis.
Abstract Introduction Ketamine is commonly used as a continuous infusion to provide sedation and analgesia in critically ill patients. Data comparing sedative opioid usage ketamine non-ketamine patient cohorts limited the burn literature. We hypothesized that patients receiving infusions (CKI) also receive less opioids over course of their hospitalization. Methods This single-center retrospective review adult admitted an ABA-verified Burn center. The first cohort (n=10) spans June 2022—May...
Neonates, especially those born prematurely, are highly vulnerable to infection-induced mortality. Numerous observational and immunological studies in newborns have shown that live attenuated vaccines beneficial, non-specific effects (NSEs) against secondary infections unrelated pathogens. These beneficial been attributed trained immunity, emergency granulopoiesis plays an essential role. However, immunity has affect multiple myeloid subsets how influences the host protective response is...
Epidemiological evidence has revealed an associative link between sepsis survivorship and increased risk of dementia, particularly Alzheimer's disease (AD). Paradoxically, population studies show females are less susceptible to but more vulnerable post-sepsis dementia. Here, we examined the temporal impacts in context AD by using AD-amyloidosis model (TgCRND8) their wild-type littermates assessing outcomes at 7 days 3 months male female mice. Following 7-days recovery, microglia astrocytes...
Introduction Sepsis engenders distinct host immunologic changes that include the expansion of myeloid-derived suppressor cells (MDSCs). These play a physiologic role in tempering acute inflammatory responses but can persist patients who develop chronic critical illness. Methods Cellular Indexing Transcriptomes and Epitopes by Sequencing transcriptomic analysis are used to describe MDSC subpopulations based on differential gene expression, RNA velocities, biologic process clustering. Results...
BACKGROUND. Sepsis remains a major clinical challenge for which successful treatment requires greater precision in identifying patients at increased risk of adverse outcomes requiring different therapeutic approaches. Predicting and immunological endotyping septic has generally relied on using blood protein or mRNA biomarkers, static cell phenotyping. Here, we sought to determine whether functional immune responsiveness would yield improved precision.
Introduction Sepsis is a complex clinical syndrome characterized by heterogenous host immune response. Historically, static protein and transcriptomic metrics have been employed to describe the underlying biology. Here, we tested hypothesis that ex vivo functional TNF expression as well an immunologic endotype based on both IFNγ could be used model outcomes in sepsis patients. Methods This prospective, observational study of patient samples collected from SPIES consortium included patients...
Central tolerance checkpoints are critical for the elimination of autoreactive B cells and prevention autoimmunity. When encounter their Ag at immature cell stage, BCR cross-linking induces receptor editing, followed by apoptosis if edited remain autoreactive. Although transcription factor Foxo1 is known to promote role related Foxo3 in central poorly understood. We find that BCR-stimulated from Foxo3-deficient mice demonstrate reduced compared with wild type cells. Despite this, Foxo3-/- do...
Introduction: Tracheostomy is an emergency procedure; however, 85% of cases are due to malignant neoplasms. For different types neoplasia, till date there no algorithms in literatures for the management airway obstruction. Objective study: To Generate algorithm obstruction head and neck cancer patients. Material methods: An exhaustive bibliographical search was carried out PubMed database with MeSH terms “malignant obstruction/surgery”, “airway obstruction/etiology”, management/ standards”....
INTRODUCTION Severe trauma disrupts bone marrow function and is associated with persistent anemia altered hematopoiesis. Previously, plasma-derived exosomes isolated after have been shown to suppress in vitro function. However, the cargo contained these vesicles has not examined. We hypothesized that plasma–derived exhibit microRNA (miRNA) changes impact severe injury. METHODS Plasma was collected from a prospective cohort study of patients (n = 15; 7 males, 8 females) hip and/or femur...
Abstract Sepsis engenders distinct host immunologic changes that include the expansion of myeloid-derived suppressor cells (MDSCs). These play a physiologic role in tempering acute inflammatory responses but can persist patients who develop chronic critical illness. The origins and lineage these MDSC subpopulations were previously assumed to be discrete unidirectional; however, exhibit dynamic phenotype with considerable plasticity. Using Cellular Indexing Transcriptomes Epitopes by...
Abstract Introduction Burn patients require significant blood products during their hospitalization. Blood is a critical and limited resource. Up to one-third of are transfused intraoperatively. Prior research identified amount tissue excised, hemoglobin, INR, platelets as predictive transfusions fresh frozen plasma (FFP) red cells (RBCs). However, development model based on both non-transfused intraoperatively has not been performed. Predicting patient’s likelihood requiring transfusion,...
Postsepsis early mortality is being replaced by survivors who experience either a rapid recovery and favorable hospital discharge or the development of chronic critical illness with suboptimal outcomes. The underlying immunological response that determines these clinical trajectories remains poorly defined at transcriptomic level. As classical nonclassical monocytes are key leukocytes in both innate adaptive immune systems, we sought to delineate cell types. Using single-cell RNA sequencing...
ABSTRACT We report a management strategy for disseminated Fusarium solani fungal infection in an adult 35% total body surface area burn patient with brain abscesses and concomitant pulmonic valve endocarditis resulting the longest survival reported patient. Early his hospital course, was diagnosed wound fungemia treated prolonged course of intravenous (IV) antifungal monotherapy. Shortly thereafter, he developed focal neurologic deficits found to have on MRI. He underwent emergent craniotomy...
Despite continued improvement in post-sepsis survival, long term morbidity and mortality remain high. Chronic critical illness (CCI), defined as persistent inflammation organ injury requiring prolonged intensive care, is a harbinger of poor long-term outcomes sepsis survivors. Current dogma states that survivors are immunosuppressed, particularly CCI. Investigation this immune suppression heterogeneous populations across distinct clinical trajectories outcomes, along with limited sampling...
ABSTRACT Sepsis, a dysregulated host immune response to infection, is one of the leading causes neonatal mortality worldwide. Improved understanding perinatal system critical improve therapies both term and preterm neonates at increased risk sepsis. Our narrative outlines known unknown aspects human through tolerant in utero period rapidly changing antigen-rich after birth. We will highlight key differences innate adaptive immunity noted these developmental stages how unique phenotype early...