A5032493640- Oral microbiology and periodontitis research
- Immune Response and Inflammation
- Pancreatic and Hepatic Oncology Research
- Extracellular vesicles in disease
- Oral Health Pathology and Treatment
- Cancer, Stress, Anesthesia, and Immune Response
- Salivary Gland Disorders and Functions
- Wound Healing and Treatments
- Burn Injury Management and Outcomes
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Immune cells in cancer
- Pain Mechanisms and Treatments
- Streptococcal Infections and Treatments
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- SARS-CoV-2 and COVID-19 Research
- Thermal Regulation in Medicine
- Sepsis Diagnosis and Treatment
- Endodontics and Root Canal Treatments
- Bone and Dental Protein Studies
- Proteoglycans and glycosaminoglycans research
- 3D Printing in Biomedical Research
- Exercise and Physiological Responses
- Dental materials and restorations
University of Florida
2016-2025
University of North Carolina at Chapel Hill
2020-2024
California University of Pennsylvania
2024
Florida College
2011-2024
University of North Carolina Health Care
2022
North Carolina Central University
2022
Queen Mary University of London
2022
University of North Carolina System
2020
East Carolina University
2018-2019
University of Florida Health Science Center
2015-2017
The cell tropism of human noroviruses and the development an in vitro infection model remain elusive. Although susceptibility to individual norovirus strains correlates with individual's histo-blood group antigen (HBGA) profile, biological basis this restriction is unknown. We demonstrate that mouse infected B cells likely vivo. Human required presence HBGA-expressing enteric bacteria. Furthermore, replication was reduced vivo when intestinal microbiota depleted by means oral antibiotic...
Despite signs of infection—including taste loss, dry mouth and mucosal lesions such as ulcerations, enanthema macules—the involvement the oral cavity in coronavirus disease 2019 (COVID-19) is poorly understood. To address this, we generated analyzed two single-cell RNA sequencing datasets human minor salivary glands gingiva (9 samples, 13,824 cells), identifying 50 cell clusters. Using integrated normalization annotation, classified 34 unique subpopulations between gingiva. Severe acute...
Sepsis, the systemic inflammatory response to microbial infection, induces changes in both innate and adaptive immunity that presumably lead increased susceptibility secondary infections, multiorgan failure, death. Using a model of murine polymicrobial sepsis whose severity approximates human sepsis, we examined outcomes defined requirements for survival after Pseudomonas aeruginosa pneumonia or disseminated Listeria monocytogenes infection. We demonstrate early neutrophil numbers function...
Background: The geometry of the fixture–abutment interface (FAI) might influence risk bacterial invasion internal part implant. aim this study was to use an in vitro model assess potential for oral microorganisms into FAI microgap dental implants with different characteristics connection between fixture and abutment. Methods: Thirty were divided three groups (n = 10 per group) based on their dynamics. Groups 1 2 comprised fixtures Morse‐taper connections that connected standard abutments...
Direct implantation of viable surgical specimens provides a representative preclinical platform in pancreatic adenocarcinoma. Patient-derived xenografts consistently demonstrate retained tumor morphology and genetic stability. However, the evolution microenvironment over time remains poorly characterized these models. This work specifically addresses recruitment incorporation murine stromal elements into expanding patient-derived adenocarcinoma xenografts, establishing integration cells...
Highlights•In silico predictions of miR-2392 as a miRNA involved with SARS-CoV-2•Overexpression produces similar biological response COVID-19 infection•miR-2392 is confirmed to circulate in serum and urine patients COVID-19•Development initiated potential antiviral therapeutic against COVID-19SummaryMicroRNAs (miRNAs) are small non-coding RNAs post-transcriptional gene regulation that have major impact on many diseases provide an exciting avenue toward therapeutics. From patient...
Abstract Background Sepsis and trauma are known to disrupt gut bacterial microbiome communities, but the impacts perturbations in fungal (mycobiome) community after severe infection or injury, particularly patients experiencing chronic critical illness (CCI), remain unstudied. Methods We assess persistence of mycobiome perturbation (dysbiosis) CCI following sepsis for up two-to-three weeks intensive care unit hospitalization. Results show that dysbiotic arrays shift toward a pathobiome...
Periodontitis affects billions of people worldwide. To address relationships periodontal niche cell types and microbes in periodontitis, we generated an integrated single-cell RNA sequencing (scRNAseq) atlas human periodontium (34-sample, 105918-cell), including sulcular junctional keratinocytes (SK/JKs). SK/JKs displayed altered differentiation states were enriched for effector cytokines periodontitis. Single-cell metagenomics revealed 37 bacterial species with cell-specific tropism....
Bioadhesive materials and patches are promising alternatives to surgical sutures staples. However, many existing bioadhesives do not meet the functional requirements of current procedures interventions. Here, we present a translational patch material that exhibits instant adhesion tissues (2.5-fold stronger than Tisseel, an FDA-approved fibrin glue), ultra-stretchability (stretching >300% its original length without losing elasticity), compatibility with rapid photo-projection (<2 min...
Background: Previously, we demonstrated that the geometry of fixture–abutment interface influences risk bacterial invasion into internal part implant, although contribution loading on this was not evaluated. The aim present study is to use an in vitro dynamic‐loading model assess potential for oral microorganisms microgap dental implants with different connection characteristics. Methods: Twenty‐eight were divided two groups (n = 14 per group) based their dynamics. Group 1 comprised fixtures...
While much research has focused on local and systemic factors contributing to periodontal disease, little is known regarding mechanisms linking these factors. We have previously reported a hyper-inflammatory response bacterial endotoxin in localized aggressive periodontitis (LAP). The objectives of this study were delineate cyto/chemokines gingival crevicular fluid (GCF) evaluate levels associated with LAP. Clinical parameters, GCF, peripheral blood collected from: 34 LAP, 10 healthy...
Skeletal muscles produce and contribute to circulating levels of IL-6 during exercise. However, when core temperature is reduced, the response attenuated. Therefore, we hypothesized that hyperthermia may be an important independent stimulus for muscle IL-6. In cultured C2C12 myotubes, (42°C) increased gene expression 14-fold after 1 h 35-fold 5 37°C recovery; whereas exposure 41°C resulted in a 2.6-fold elevation at h. protein was secreted significantly elevated cell supernatant. Similar but...
The ‘hyper-responsive’ trait is an increased inflammatory response upon stimulation of innate immune receptors. Our objective was to determine if a hyper-reactive present in cohort diagnosed with aggressive periodontitis (LAgP). Peripheral blood collected from 30 LAgP, 10 healthy unrelated, and sibling participants stimulated lipopolysaccharide (LPS) E. coli P. gingivalis. Cyto/chemokine profiles were evaluated analyzed by ANOVA. Elevated levels pro-inflammatory cyto/chemokines detected...
We have reported a lipopolysaccharide (LPS)-induced hyper-inflammatory response in localized aggressive periodontitis (LAP). It is unknown whether treatment able to modulate this LPS responsiveness. Fifty-nine individuals with LAP were treated by mechanical debridement and systemic antibiotics. Clinical parameters cyto/chemokine responsiveness of whole blood stimulated Porphyromonas gingivalis or Escherichia coli monitored at baseline 3, 6, 12 months post-treatment. Overall, clinical...
Both epidemiologic and experimental findings suggest that infection with Porphyromonas gingivalis exacerbates progression of atherosclerosis. As P. exhibits significant strain variation, it is reasonable different strains possess capabilities and/or mechanisms by which they promote Using have been previously evaluated in the ApoE null atherosclerosis model, we assessed ability W83, A7436, 381, 33277 to adhere, invade, persist human coronary artery endothelial (HCAE) cells. W83 381 displayed...
The objective of this case-control study was to quantify the immune responsiveness in individuals with type 2 diabetes (T2D) as compared patients without (NT2D) diagnosed periodontitis.Peripheral blood collected from 20 moderate-to-severe chronic periodontitis (10 T2D, 10 NT2D). Blood samples were stimulated ultrapure Porphyromonas gingivalis and Escherichia coli lipopolysaccharide (LPS) for 24 hours. 14 cytokines/chemokines quantified culture supernatants using multiplex technology.T2D...
Bacterial colonization of the fixture-abutment interface (FAI) microgap may contribute to increased marginal bone loss. The contribution loading on bacterial has not been thoroughly evaluated with in vitro experiments. aim this study was evaluate effect dynamic oral microorganisms FAI dental implants internal Morse-taper connection. Forty were divided into two groups (n = 20/group) based subjection conditions. Both Group 1 and 2 comprised fixtures that connected standard abutments allowed...
Heat stroke afflicts thousands of humans each year, worldwide. The immune system responds to hyperthermia exposure resulting in heat by producing an array immunological proteins, such as interleukin-6 (IL-6). However, the physiological functions IL-6 and other cytokines are poorly understood. We hypothesized that plays a protective role conditions stroke. To test this, we gave small supplements mice prior exposing them hot environments sufficient induce Pretreatment with resulted improved...
Abstract This study investigates the role of NLRP3 inflammasome and its main effector Caspase-1 in inflammation alveolar bone resorption associated with periodontitis. Heat-killed Aggregatibacter actinomycetemcomitans (Aa) was injected 3x/week (4 weeks) into gingival tissues wild-type (WT), Nlrp3 -KO Caspase1 mice. Bone measured by µCT osteoclast number determined tartrate-resistant acid phosphatase (TRAP) staining. Inflammation assessed histologically (H/E staining immunofluorescence CD45...
ABSTRACT Despite signs of infection, the involvement oral cavity in COVID-19 is poorly understood. To address this, single-cell RNA sequencing data-sets were integrated from human minor salivary glands and gingiva to identify 11 epithelial, 7 mesenchymal, 15 immune cell clusters. Analysis SARS-CoV-2 viral entry factor expression showed enrichment epithelia including ducts acini suprabasal cells mucosae. autopsy tissues confirmed vivo infection mucosa. Saliva SARS-CoV-2-infected individuals...
The treatment of chronic inflammation with systemically administered anti-inflammatory treatments is associated moderate-to-severe side effects, and the efficacy locally drugs short-lived. Here we show that can be suppressed by a fusion protein immunosuppressive enzyme indoleamine 2,3-dioxygenase 1 (IDO) galectin-3 (Gal3). Gal3 anchors IDO to tissue, limiting diffusion IDO-Gal3 away from injection site. In rodent models endotoxin-induced inflammation, psoriasis, periodontal disease...