Sabrina Ramelli
A5057273511- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Immune Cell Function and Interaction
- Asthma and respiratory diseases
- MicroRNA in disease regulation
- COVID-19 Impact on Reproduction
- Vaccine Coverage and Hesitancy
- Circular RNAs in diseases
- Pneumonia and Respiratory Infections
- Inflammasome and immune disorders
- Antimicrobial Peptides and Activities
- Viral gastroenteritis research and epidemiology
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Dermatological and COVID-19 studies
- Immune Response and Inflammation
- SARS-CoV-2 detection and testing
- Heparin-Induced Thrombocytopenia and Thrombosis
- Immune cells in cancer
- Respiratory viral infections research
- CRISPR and Genetic Engineering
- Retinal and Optic Conditions
- Oral Health Pathology and Treatment
- Kawasaki Disease and Coronary Complications
- Hydrogen's biological and therapeutic effects
National Institutes of Health Clinical Center
2019-2024
National Institutes of Health
2019-2024
Government of the United States of America
2024
National Heart Lung and Blood Institute
2024
National Cancer Institute
2023
Center for Cancer Research
2023
National Institute of Allergy and Infectious Diseases
2023
University of South Alabama
2018-2020
Despite signs of infection—including taste loss, dry mouth and mucosal lesions such as ulcerations, enanthema macules—the involvement the oral cavity in coronavirus disease 2019 (COVID-19) is poorly understood. To address this, we generated analyzed two single-cell RNA sequencing datasets human minor salivary glands gingiva (9 samples, 13,824 cells), identifying 50 cell clusters. Using integrated normalization annotation, classified 34 unique subpopulations between gingiva. Severe acute...
Abstract COVID-19 is known to cause multi-organ dysfunction 1-3 in acute infection, with prolonged symptoms experienced by some patients, termed Post-Acute Sequelae of SARS-CoV-2 (PASC) 4-5 . However, the burden infection outside respiratory tract and time viral clearance not well characterized, particularly brain 3,6-14 We performed complete autopsies on 44 patients map quantify distribution, replication, cell-type specificity across human body, including brain, from through over seven...
Tracking evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within infected individuals will help elucidate disease 2019 (COVID-19) pathogenesis and inform use antiviral interventions. In this study, we developed an approach for sequencing region encoding SARS-CoV-2 virion surface proteins from large numbers individual virus RNA genomes per sample. We applied to WA-1 reference clinical isolate passaged in vitro upper samples 7 study participants with COVID-19. cell...
In order to assess homeostatic mechanisms in the lung after COVID-19, changes protein signature of bronchoalveolar lavage from 45 patients with mild moderate disease at three phases (acute, recovery, and convalescent) are evaluated over a year. During acute phase, inflamed uninflamed phenotypes characterized by expression tissue repair host defense response molecules. With inflammatory fibrogenic mediators decline clinical symptoms abate. However, 9 months, quantified radiographic...
A 26-year-old otherwise healthy man died of fulminant myocarditis. Nasopharyngeal specimens collected premortem tested negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Histopathological evaluation the heart showed myocardial necrosis surrounded by cytotoxic T-cells and tissue-repair macrophages. Myocardial T-cell receptor (TCR) sequencing revealed hyper-dominant clones with highly similar sequences to TCRs that are specific SARS-CoV-2 epitopes. RNA was detected in...
Background Although there have been many studies on antibody responses to SARS-CoV-2 in breast milk, very few looked at the fate of these infant, and whether they are delivered immunologically relevant sites infants. Methods Mother/infant pairs (mothers who milk fed were vaccinated before or after delivery) recruited for this cross-sectional study. Mother blood, mother infant nasal specimen, stool was tested IgA IgG antibodies against spike trimer. Results Thirty-one mother/infant recruited....
Saliva contains antimicrobial peptides considered integral components of host innate immunity, and crucial for protection against colonizing microbial species. Most notable is histatin-5 which exclusively produced in salivary glands with uniquely potent antifungal activity the opportunistic pathogen Candida albicans . Recently, SARS-CoV-2 was shown to replicate gland acinar cells eliciting local immune cell activation. In this study, we performed studies investigate implications infection on...
Abstract Tracking evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within infected individuals will help elucidate disease 2019 (COVID-19) pathogenesis and inform use antiviral interventions. In this study, we developed an approach for sequencing region encoding SARS-CoV-2 virion surface proteins from large numbers individual virus RNA genomes per sample. We applied to WA-1 reference clinical isolate passaged in vitro upper samples 7 study participants with...
Saliva contains antimicrobial peptides considered integral components of host innate immunity, and crucial for protection against colonizing microbial species. Most notable is histatin-5 which exclusively produced in salivary glands with uniquely potent antifungal activity the opportunistic pathogen
Understanding early innate immune responses to coronavirus disease 2019 (COVID-19) is crucial developing targeted therapies mitigate severity. Severe acute respiratory syndrome (SARS-CoV)-2 infection elicits interferon expression leading transcription of IFN-stimulated genes (ISGs) control viral replication and spread. SARS-CoV-2 also NF-κB signaling which regulates inflammatory cytokine contributing likely Few studies have simultaneously characterized these two components immunity COVID-19....
In order to assess the restoration of homeostasis in lung proteome after COVID-19 infection, we performed bronchoalveolar lavage on 45 patients with mild moderate disease at three phases (acute, recovery, convalescence) over a year. Changes proteins were assessed using multimodal approach. During acute phase, inflamed and uninflamed phenotypes characterized by expression tissue repair host defense response molecules. With inflammatory fibrogenic mediators declined clinical symptoms abated....
ABSTRACT Nonhuman primate models that closely emulate the disease course, pathogenesis, and supportive care provided to human patients in modern intensive unit with bacterial sepsis are urgently needed study pathogenesis assess novel therapies. We therefore developed a non-human model of septic shock includes akin unit. In this study, we characterized pathogen kinetics evaluated physiologic, immunologic, pathologic responses induced by clinically relevant Klebsiella pneumoniae across...
Bacterial and fungal co-infections are reported complications of coronavirus disease 2019 (COVID-19) in critically ill patients but may go unrecognized premortem due to diagnostic limitations. We compared the with postmortem detection pulmonary 55 fatal COVID-19 cases from March 2020 2021. The concordance versus diagnoses pathogen identification were evaluated. Premortem extracted medical charts while applying standard definitions. Postmortem co-infection was defined by compatible lung...
SARS-CoV-2 infection triggers adaptive immune responses from both T and B cells. However, most studies focus on peripheral blood, which may not fully reflect in lymphoid tissues at the site of infection. To evaluate local systemic to SARS-CoV-2, we collected tonsils, adenoids 110 children undergoing tonsillectomy/adenoidectomy during COVID-19 pandemic found 24 with evidence prior infection, including detectable neutralizing antibodies against multiple viral variants. We identified...
Platt, Andrew1; Nasir, Nadia2; Ben, Bradley3; Stein, Sydney4; Ramelli, Sabrina4; Ramos-Benitez, Marcos4; Dickey, James1; Purcell, Madeleine5; Wu, Jocelyn5; Singireddy, Shreya5; Salipante, Stephen3; Chisholm, Claire3; Grazioli, Alison6; Madathil, Ronson5; Rabin, Joseph5; Saharia, Kapil5; Kleiner, David2; Hewitt, Stephen2; Leiberman, Joshua3; Chertow, Daniel1 Author Information
Abstract Background T-cells play an essential role in recognizing and clearing viruses from infected tissues. While the peripheral blood T-cell responses among patients with coronavirus disease 2019 (COVID-19) has been well characterized recent literature, little is known about overall repertoire diversity of SARS-CoV-2-specific breadth depth across tissues severe COVID-19. To fill this knowledge gap, we evaluated immune repertoires using bulk sequencing multiple tissue samples a cohort...