Anish Kaachra

ORCID: 0000-0003-2381-5467
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About
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Research Areas
  • Plant nutrient uptake and metabolism
  • Biochemical and biochemical processes
  • Plant biochemistry and biosynthesis
  • Photosynthetic Processes and Mechanisms
  • Metal-Catalyzed Oxygenation Mechanisms
  • Cholinesterase and Neurodegenerative Diseases
  • Plant Molecular Biology Research
  • Plant Gene Expression Analysis
  • Plant Stress Responses and Tolerance
  • Insect-Plant Interactions and Control
  • Mass Spectrometry Techniques and Applications
  • Legume Nitrogen Fixing Symbiosis
  • Signaling Pathways in Disease
  • Plant Virus Research Studies
  • Bioactive Compounds and Antitumor Agents
  • Plant-Microbe Interactions and Immunity
  • Plant tissue culture and regeneration
  • Toxin Mechanisms and Immunotoxins
  • Phytochemical compounds biological activities
  • Peptidase Inhibition and Analysis
  • Plant Reproductive Biology
  • Plant Physiology and Cultivation Studies

Institute of Himalayan Bioresource Technology
2010-2024

Academy of Scientific and Innovative Research
2018-2024

Council of Scientific and Industrial Research
2010-2022

Tracer experiments using 13CO2 is a valuable tool for studying the metabolic flux of carbon during photosynthesis, providing precise insights into assimilation and distribution within plant systems. However, complexity designing setting up delivery system significant factor limiting use tracer in many research laboratories. In present work, we report simple that can be used conjunction with an Infrared gas analyser (IRGA) short duration vivo 13C labelling leaves. Briefly, released external...

10.1101/2025.03.18.643862 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2025-03-19

Abstract Background Geranyl pyrophosphate (GPP) and p -hydroxybenzoate (PHB) are the basic precursors involved in shikonins biosynthesis. GPP is derived from mevalonate (MVA) and/or 2- C -methyl-D-erythritol 4-phosphate (MEP) pathway(s), depending upon metabolite plant system under consideration. PHB, however, synthesized by only phenylpropanoid (PP) pathway. PHB central moieties to yield through synthesis of m -geranyl- (GHB). Enzyme -hydroxybenzoate- -geranyltransferase (PGT) catalyses...

10.1186/1471-2199-11-88 article EN cc-by BMC Molecular Biology 2010-11-21

We investigated the effect of heterologous expression phosphoenolpyruvate carboxylase (ZmPepcase), aspartate aminotransferase (GmAspAT), and glutamine synthetase (NtGS) on carbon (C) nitrogen (N) metabolism in Arabidopsis (Arabidopsis thaliana). These transgenes were expressed either separately or different combinations. The highest gains shoot dry weight observed transgenic lines coexpressing all three genes. Tracer experiments using NaH14CO3 suggested that coexpression ZmPepcase, GmAspAT,...

10.1104/pp.18.00379 article EN PLANT PHYSIOLOGY 2018-06-11

Abstract The estimation of relative levels amino acids is crucial for understanding various biological processes in plants, including photosynthesis, stress tolerance, and the uptake translocation nutrients. A wide range liquid chromatography (LC; HPLC/UHPLC) -based methods available measuring quantity plants. Additionally, coupling LC with mass spectrometry (MS) significantly enhanced robustness existing chromatographic used acid quantification. However, accurate annotation integration...

10.1101/2024.06.28.601148 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2024-07-02

ABSTRACT The estimation of relative levels amino acids is crucial for understanding various biological processes in plants, including photosynthesis, stress tolerance, and the uptake translocation nutrients. A wide range liquid chromatography (LC; HPLC/UHPLC)‐based methods available measuring quantity plants. Additionally, coupling LC with mass spectrometry (MS) significantly enhanced robustness existing chromatographic used acid quantification. However, accurate annotation integration peaks...

10.1002/jms.5094 article EN Journal of Mass Spectrometry 2024-09-25

Peptidyl-prolyl cis-trans isomerases (PPIases) are ubiquitous proteins which essential for isomerisation of peptide bonds preceding the proline residue. PPIases categorized into four sub-families viz ., cyclophilins, FK506-binding (FKBPs), parvulins and protein phosphatase 2A activators (PTPAs). Apart from catalysing isomerization, these have also been implicated in diverse cellular functions. Though identified several important crop plants, information on proteins, except is scanty wheat....

10.3389/fpls.2022.1053524 article EN cc-by Frontiers in Plant Science 2022-12-15

Abstract Plant scientists across the globe are interested in studying root growth architecture of plants to understand different processes determining and development plants. In majority cases, growth-related experiments carried out on petri plates filled with solid nutrient media. However, growing these often exposed conditions that not close natural conditions. Also, it is difficult pour two media same plate which quite a useful feature study effect specific treatment plant growth. present...

10.21203/rs.3.rs-3219193/v1 preprint EN cc-by Research Square (Research Square) 2023-08-01
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