Pseudomonas aeruginosa is one of the most commonly isolated bacteria from clinical specimens, with an increasing isolation frequency in nosocomial outbreaks. The hypothesis tested was whether carbapenem-resistant P. strains display altered carriage virulence factor genes, depending on type carbapenem resistance. aim study to investigate, by PCR, 10 chosen factors genes (phzM, phzS, exoT, exoY, exoU, toxA, exoS, algD, pilA and pilB) genotype distribution 107 non-duplicated isolates. involved...

Currently used methods for diagnosing ventilator-associated pneumonia (VAP) are complex, time-consuming and require invasive procedures while empirical antibacterial therapy applies broad spectrum antibiotics that may promote antimicrobial resistance. Hence, novel fast based on alternative markers needed VAP detection differentiation of causative pathogens. Pathogenic bacteria produce a range volatile organic compounds (VOCs), some which potentially serve as biomarkers microorganism...

Viral infections, or their reactivations, are one of the most important groups transplantation complications that can occur among recipients both hematopoietic cells and solid organ transplants. They commonly caused by cytomegalovirus (CMV). Currently, use whole blood plasma samples is recommended for CMV viral load monitoring. The aim study was to assess compare level DNA, depending on type clinical material—whole fraction derived from same patient. studies were carried out 156 in which...

Epstein-Barr virus (EBV) is an oncogenic classified by the World Health Organization as a class 1 carcinogen. Post-transplant lymphoproliferative disorders are believed to be strongly related EBV infection. Monitoring of DNAemia recommended assess risk reactivation latent infection and effectiveness therapy. Currently, various types clinical specimens used for this purpose. The aim study was reliable method viral load investigation depending on material used: whole blood or plasma samples....

Bacterial infection of the lower respiratory tract frequently occurs in mechanically ventilated patients and may develop into life-threatening conditions. Yet, existing diagnostic methods have moderate sensitivity specificity, which results overuse broad-spectrum antibiotics administered prophylactically. This study aims to evaluate suitability volatile bacterial metabolites for breath-based test, is used diagnosing Ventilator-Associated Pneumonia (VAP). The vitro experiments with pathogenic...

Pseudomonas aeruginosa is an opportunistic pathogen encoding several virulence factors in its genome, which well-known for ability to cause severe and life-threatening infections, particularly among cystic fibrosis patients. The organism also a major of nosocomial mainly affecting patients with immune deficiencies burn wounds, ventilator-assisted patients, affected by other malignancies. extensively reported emergence multidrug-resistant (MDR) P. strains poses additional challenges the...

Healthcare-associated infections caused by multidrug-resistant Acinetobacter baumannii strains are a serious global threat. Therefore, it is important to expand the knowledge on mechanisms of pathogenicity these particular bacteria. The aim this study was assess distribution selected virulence factor genes (bap, surA1, omp33-36, bauA, bauS, and pld) among carbapenem-non-susceptible clinical A. isolates evaluate their potential usefulness as genetic markers for rapid diagnostics infections....

Multidrug-resistant Acinetobacter baumannii, particularly those producing carbapenemases, are spread worldwide. A reliable detection of carbapenemases is essential to choose the appropriate antimicrobial therapy and, consequently, prevent dissemination carbapenem-resistant strains. The aim this study examine molecular basis carbapenem resistance mechanism and estimation conventional PCR real-time usefulness for oxacillinases when compared phenotypic detection. following methods were...

Pseudomonas aeruginosa is a Gram-negative rod and an etiological factor of opportunistic infections. The infections this etiology appear mostly among hospitalized patients are relatively hard to treat due widespread antimicrobial resistance. Many virulence factors involved in the pathogenesis P. infection, coexistence which have significant impact on course infection with particular localization. aim study was assess susceptibility profiles frequency genes encoding selected clinical strains...

SARS-CoV-2 is a new virus from the Coronaviridae family and its rapid spread now most important medical problem worldwide. Currently used tests vary in number selection of target genes. Meanwhile, choice appropriate gene may be terms reliable detection viral RNA. As some researchers questioned sensitivity monogenic VIASURE S Real Time PCR Detection Kit (CerTest Biotec, Zaragoza, Spain) mid-2020, aim study was to evaluate usefulness this kit, along with BD MAX™ System (Becton Dickinson, East...

Carbapenem-resistant

Bacterial infection of the lower respiratory tract frequently occurs in mechanically ventilated pa-tients and may develop into life-threatening conditions. Yet existing diagnostic methods have moderate sensitivity specificity, which results overuse broad-spectrum antibiotics administrated prophylactically. This study aims to evaluate suitability volatile bacterial me-tabolites for breath-based test diagnosing Ventilation-Associated Pneumonia (VAP). The vitro experiments with pathogenic...

BackgroundStreptococcus agalactiae (group B streptococcus: GBS) is a leading cause of early- and late-onset diseases in neonates. Reliable results GBS carriage investigation among pregnant women may decrease the incidence neonatal infection mortality.AimTo compare conventional culture with those US Food Drug Administration-approved nucleic acid amplification test (BD Max (Becton Dickinson)), to establish our own protocols standard polymerase chain reaction (PCR).MethodsA total 250...

Abstract Given the dynamic growth of antibiotic resistance observed over past few decades, new therapeutic methods for life-threatening infections are crucial. Effective treatment caused by multidrug-resistant microorganisms increasingly requires use last resort drugs, and antibiotics generate in less time than it takes to bring them therapy. Therefore is advisable seek solutions using currently available antibiotics. therapy with may be enabled additive synergistic interactions, resulting...