A5089482642- Advanced Chemical Sensor Technologies
- Analytical Chemistry and Chromatography
- Olfactory and Sensory Function Studies
- Metabolomics and Mass Spectrometry Studies
- Insect Pheromone Research and Control
- Mass Spectrometry Techniques and Applications
- Gas Sensing Nanomaterials and Sensors
- Essential Oils and Antimicrobial Activity
- Meat and Animal Product Quality
- Biochemical Analysis and Sensing Techniques
- Nosocomial Infections in ICU
- Probiotics and Fermented Foods
- Biosensors and Analytical Detection
- Inhalation and Respiratory Drug Delivery
- Respiratory and Cough-Related Research
- Antibiotic Resistance in Bacteria
- Protein Hydrolysis and Bioactive Peptides
- Pharmacological Effects and Toxicity Studies
- Antibiotics Pharmacokinetics and Efficacy
- Gut microbiota and health
- Phytochemicals and Antioxidant Activities
- Hormonal and reproductive studies
- Natural product bioactivities and synthesis
- Ion channel regulation and function
- Microfluidic and Capillary Electrophoresis Applications
Collegium Medicum in Bydgoszcz
2017-2025
Nicolaus Copernicus University
2007-2025
University of Bydgoszcz
2019
Universität Innsbruck
2013-2017
Innsbruck Medical University
2008-2016
Austrian Academy of Sciences
2008-2013
University of Rostock
2010
Abstract Background Lung cancer is one of the leading causes death in Europe and western world. At present, diagnosis lung very often happens late course disease since inexpensive, non-invasive sufficiently sensitive specific screening methods are not available. Even though CT diagnostic good, it must be assured that "screening benefit outweighs risk, across all individuals screened, only those with cancer". An early would improve prognosis enlarge treatment options. Analysis exhaled breath...
Abstract Background The routinely used microbiological diagnosis of ventilator associated pneumonia (VAP) is time consuming and often requires invasive methods for collection human specimens (e.g. bronchoscopy). Therefore, it utmost interest to develop a non-invasive method the early detection bacterial infection in ventilated patients, preferably allowing identification specific pathogens. present work an attempt identify pathogen-derived volatile biomarkers breath that can be non- (VAP)....
Abstract The aim of this study was to confirm the existence volatile organic compounds (VOC) specifically released or consumed by lung cancer cell line A549, which could be used in future screens as biomarkers for early detection cancer. For comparison, primary human bronchial epithelial cells (HBEpC) and fibroblasts (hFB) were included. VOCs detected headspace cultures medium controls following adsorption on solid sorbents, thermodesorption, analysis gas chromatography mass spectrometry....
Non-invasive disease monitoring on the basis of volatile breath markers is a very attractive but challenging task. Several hundreds compounds have been detected in exhaled air using modern analytical techniques (e.g. proton-transfer reaction mass spectrometry, gas chromatography-mass spectrometry) and even linked to various diseases. However, biochemical background for most samples has not elucidated; therefore, obtained results should be interpreted with care avoid false correlations. The...
The aim of this work was to confirm the existence volatile organic compounds (VOCs) specifically released or consumed by lung cancer cells.50 million cells human non-small cell (NSCLC) line CALU-1 were incubated in a sealed fermenter for 4 h over night (18 hours). Then air samples from headspace culture vessel collected and preconcentrated adsorption on solid sorbents with subsequent thermodesorption analysis means gas chromatography mass spectrometry (GC-MS). Identification altogether 60...
In this phenomenological study we focus on dynamic measurements of volatile organic compounds (VOCs) in exhaled breath under exercise conditions. An experimental setup efficiently combining breath-by-breath analyses using proton transfer reaction mass spectrometry (PTR-MS) with data reflecting the behaviour major hemodynamic and respiratory parameters is presented. Furthermore, a methodology for complementing continuous VOC profiles obtained by PTR-MS simultaneous SPME/GC-MS outlined. These...
The present study was performed to determine the variations of breath acetone concentrations with age, gender and body-mass index (BMI). Previous investigations were based on a relatively small cohort subjects (see Turner et al 2006 Physiol. Meas. 27 321-37). Since exhaled analysis is affected by considerable variation, larger studies are needed get reliable information about correlation volatiles in when compared BMI. Mixed expiratory sampled using Tedlar bags. mass-to-charge ratio (m/z)...
Breath analysis for the purpose of non-invasive diagnosis lung cancer has yielded numerous candidate compounds with still questionable clinical relevance. To arrive at suitable volatile organic our approach combined different sources: isolated tumor samples compared to healthy tissues, and exhaled breath from patients controls. Candidate were further substances previously identified in comparison transformed normal epithelial cell lines. For human studies, a sampling device was developed...
Proton-transfer-reaction mass spectrometry (PTR-MS) is a convenient technique for fast analysis of exhaled breath without prior sample preparation. Since compounds are not separated to as in gas chromatography (GC-MS), and since protonated molecules may fragment, relatively complex spectra arise, which easily interpreted quantitative way. We calibrated 21 different importance analysis, based on the respective pure standards diluted with nitrogen. These calibration measurements included...
Background : This study was performed to clarify variations in breath isoprene concentrations with age, gender, body mass index (BMI) and total serum cholesterol. Our cohort consisted of 205 adult volunteers different smoking background without health complaints. Total cholesterol blood measured 79 these volunteers. Methods Mixed expiratory exhaled sampled using Tedlar bags. Concentrations were then determined proton transfer reaction-mass spectrometry. Results Isoprene ranged from 5.8 274.9...
Existing methods for the early detection of infections in mechanically ventilated (MV) patients at intensive care units (ICUs) are unsatisfactory. Here we present an exploratory study assessing feasibility breath VOC analyses non-invasive pathogens lower respiratory tract patients. An open uncontrolled clinical pilot was performed by enrolling 28 with severe intracranial disease, being risk development or already confirmed ventilation-associated pneumonia (VAP). The recently developed...
Volatile organic compounds (VOCs) released from or taken up by Streptococcus pneumoniae and Haemophilus influenzae cultures were analysed means of GC-MS after adsorption headspace samples on multi-bed sorption tubes. Sampling was performed at different time points during cultivation bacteria to follow the dynamics VOC metabolism. VOCs identified not only spectral library match but also based retention times native standards. As many as 34 volatile metabolites S. 28 H. influenzae, comprising...
Analysis of volatile organic compounds (VOCs) provides an elegant approach for cancer screening and disease monitoring, whose use is currently limited by a lack validated cancer-derived metabolites, which may serve as biomarkers. The aim the ex
Chronic obstructive pulmonary disease (COPD) is a major cause of death worldwide. Acute exacerbations COPD (AECOPD), caused by infectious and non-infectious agents, contribute to an increase in mortality. The diagnostic procedure AECOPD mainly based on clinical features. aim this pilot study was identify whether volatile organic compounds (VOCs) breath could be used discriminate for acute exacerbated COPD. Three patient groups were included controlled study: patients (n = 14, age mean ± SD:...
Pathogenic microorganisms produce numerous metabolites, including volatile organic compounds (VOCs). Monitoring these metabolites in biological matrices (e.g., urine, blood, or breath) can reveal the presence of specific microorganisms, enabling early diagnosis infections and timely implementation targeted therapy. However, complex only contain trace levels VOCs, their constituent components hinder determination compounds. Therefore, modern analytical techniques non-invasive identification...
Breath gas samples from 27 patients with epilepsy (17 male and 10 female patients; mean age: 9.7 years, median 8.2 SD: ±4.2 years) were screened via proton transfer reaction mass spectrometry. The treated valproic acid (VPA) therapy, blood for determination of VPA concentrations surveyed. All showed significantly elevated 3-heptanone (C(7)H(14)O) in exhaled breath (mean: 14.7 ppb, median: 13.8 ppb ±5.7 ppb). In human breath, several hundred different volatile organic compounds can be...
The approach for breath-VOCs' collection and preconcentration by applying needle traps was developed optimized. alveolar air collected from only a few exhalations under visual control of expired CO2 into large gas-tight glass syringe then warmed up to 45 °C short time avoid condensation. Subsequently, specially constructed sampling device equipped with Bronkhorst® electronic flow controllers used automated adsorption. This allows time-saving expired/inspired in parallel onto three different...
Currently used methods for diagnosing ventilator-associated pneumonia (VAP) are complex, time-consuming and require invasive procedures while empirical antibacterial therapy applies broad spectrum antibiotics that may promote antimicrobial resistance. Hence, novel fast based on alternative markers needed VAP detection differentiation of causative pathogens. Pathogenic bacteria produce a range volatile organic compounds (VOCs), some which potentially serve as biomarkers microorganism...