A5041297788- Advanced Chemical Sensor Technologies
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Olfactory and Sensory Function Studies
- Insect Pheromone Research and Control
- Gas Sensing Nanomaterials and Sensors
- Biochemical Analysis and Sensing Techniques
- Mycobacterium research and diagnosis
- Meat and Animal Product Quality
- Analytical Chemistry and Sensors
- Gut microbiota and health
- Biosensors and Analytical Detection
- Respiratory Support and Mechanisms
- Mass Spectrometry Techniques and Applications
- Diet and metabolism studies
- Spectroscopy and Laser Applications
- Analytical chemistry methods development
- Air Quality Monitoring and Forecasting
- Microfluidic and Capillary Electrophoresis Applications
- Anesthesia and Sedative Agents
- Ginseng Biological Effects and Applications
- Clostridium difficile and Clostridium perfringens research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Infectious Diseases and Mycology
- Respiratory viral infections research
University of Rostock
2015-2024
ORCID
2021
University Medical Center
2019
Universitätsmedizin Rostock
2018
Austrian Academy of Sciences
2008-2011
Innsbruck Medical University
2010
Heinrich Heine University Düsseldorf
2008
Düsseldorf University Hospital
2008
University of Freiburg
2001
University Hospital Augsburg
2001
Abstract Background Lung cancer is one of the leading causes death in Europe and western world. At present, diagnosis lung very often happens late course disease since inexpensive, non-invasive sufficiently sensitive specific screening methods are not available. Even though CT diagnostic good, it must be assured that "screening benefit outweighs risk, across all individuals screened, only those with cancer". An early would improve prognosis enlarge treatment options. Analysis exhaled breath...
Abstract There is experimental evidence that volatile substances in human breath can reflect presence of neoplasma. Volatile aldehydes were determined exhaled 12 lung cancer patients, smokers and healthy volunteers. Alveolar samples collected under control expired CO 2 . Reactive transformed into stable oximes by means on‐fiber‐derivatization (SPME‐OFD). Aldehyde concentrations the ppt ppb level gas chromatography‐mass spectrometry (GC‐MS). Exhaled corrected for inspired values. C 1 – 10...
Background: Analysis of exhaled breath is a promising diagnostic method. Sampling non-invasive and can be performed as often considered desirable. There are indications that the concentration presence certain volatile compounds in lung cancer patients different from concentrations healthy volunteers. This might lead to future test for cancer.
A real-time recording setup combining exhaled breath volatile organic compound (VOC) measurements by proton transfer reaction-mass spectrometry (PTR-MS) with hemodynamic and respiratory data is presented. Continuous automatic sampling of implemented on the basis measured flow: a flow-controlled shutter mechanism guarantees that only end-tidal exhalation segments are drawn into mass spectrometer for analysis. Exhaled concentration profiles two prototypic compounds, isoprene acetone, during...
Abstract The aim of this study was to confirm the existence volatile organic compounds (VOC) specifically released or consumed by lung cancer cell line A549, which could be used in future screens as biomarkers for early detection cancer. For comparison, primary human bronchial epithelial cells (HBEpC) and fibroblasts (hFB) were included. VOCs detected headspace cultures medium controls following adsorption on solid sorbents, thermodesorption, analysis gas chromatography mass spectrometry....
The aim of this work was to confirm the existence volatile organic compounds (VOCs) specifically released or consumed by lung cancer cells.50 million cells human non-small cell (NSCLC) line CALU-1 were incubated in a sealed fermenter for 4 h over night (18 hours). Then air samples from headspace culture vessel collected and preconcentrated adsorption on solid sorbents with subsequent thermodesorption analysis means gas chromatography mass spectrometry (GC-MS). Identification altogether 60...
The present study was performed to determine the variations of breath acetone concentrations with age, gender and body-mass index (BMI). Previous investigations were based on a relatively small cohort subjects (see Turner et al 2006 Physiol. Meas. 27 321-37). Since exhaled analysis is affected by considerable variation, larger studies are needed get reliable information about correlation volatiles in when compared BMI. Mixed expiratory sampled using Tedlar bags. mass-to-charge ratio (m/z)...
The impact of different sampling techniques on the results breath analysis was to be assessed in this study. Alveolar, mixed expiratory and time-controlled samples were collected from ten volunteers eight lung cancer patients. Breath visually controlled by means capnometry. PCO2 13 VOCs determined. Mixed yielded 25% lower concentrations CO2 blood-borne VOCs. Time-controlled generated high variation results. Ratios Calv/Cmixed >1.5 for CO2, acetone isoprene, <1 isopropanol, 2-butanone...
Analysis of volatile organic compounds (VOCs) in breath holds great promise for noninvasive diagnostic applications. However, concentrations VOCs may change quickly, and actual previous uptakes exogenous substances, especially the clinical environment, represent crucial issues. We therefore adapted proton-transfer-reaction-time-of-flight-mass spectrometry real time analysis environment. For reasons medical safety, a 6 m long heated silcosteel transfer line connected to sterile mouth piece...
Background : This study was performed to clarify variations in breath isoprene concentrations with age, gender, body mass index (BMI) and total serum cholesterol. Our cohort consisted of 205 adult volunteers different smoking background without health complaints. Total cholesterol blood measured 79 these volunteers. Methods Mixed expiratory exhaled sampled using Tedlar bags. Concentrations were then determined proton transfer reaction-mass spectrometry. Results Isoprene ranged from 5.8 274.9...
This study was intended to evaluate low-volume (20 mL) multibed needle trap (NTD) sampling combined with heart-cut gas chromatography/mass spectrometry (GC/MS) and comprehensive two-dimensional chromatography/time-of-flight mass (GC × GC/TOF-MS) for trace analysis under clinical conditions. NTDs, high-throughput automatic desorption separation systems, were tested in vitro within a 11 patients undergoing cardiac surgery respect reproducibility, reliability, applicability. NTD−heart-cut GC/MS...
Background While assumed to protect against coronavirus transmission, face masks may have effects on respiratory–haemodynamic parameters. Within this pilot study, we investigated immediate and progressive of FFP2 surgical exhaled breath constituents physiological attributes in 30 adults at rest. Methods We continuously monitored profiles within mask space older (age 60–80 years) young middle-aged 20–59 over the period 15 min by high-resolution real-time mass-spectrometry. Peripheral oxygen...
Plants, animals and humans metabolically produce volatile isoprene (C5H8). Humans continuously exhale exhaled concentrations differ under various physio-metabolic pathophysiological conditions. Yet unknown metabolic origin hinders to reach clinical practice as a biomarker. Screening 2000 individuals from consecutive mass-spectrometric studies, we herein identify five healthy German adults without isoprene. Whole exome sequencing in these reveals only one shared homozygous (European...
To facilitate their use in trace gas analysis, the adsorption capacity of needle trap devices (NTDs) was increased by combining three adsorbent materials and increasing total amount. The 22 gauge needles, application internally expanding desorptive flow technique without cryofocusation a new on site alveolar sampling method for NTDs provided sensitivity parts per trillion range VOC concentrations loosing precision or linearity. LODs were 0.4 ng/L isoprene, 0.5 dimethyl sulphide, 0.9...
Most--if not all--potential diagnostic applications in breath research involve different marker concentrations rather than unique markers which only occur the diseased state. Hence, data interpretation is a crucial step analysis. To avoid artificial significance testing every effort should be made to implement method validation, cross-testing and statistical validation along this process. The most common analysis related problems can classified into three groups: confounding variables (CVs),...
Analysis of volatile organic compounds (VOCs) provides an elegant approach for cancer screening and disease monitoring, whose use is currently limited by a lack validated cancer-derived metabolites, which may serve as biomarkers. The aim the ex