The selection of the sample treatment strategy is a crucial step in metabolomics workflow. Solid phase microextraction (SPME) processing methodology with great potential for use untargeted tissue samples. However, its utilization not as widespread other standard protocols involving steps collection, metabolism quenching, homogenization, and extraction metabolites by solvents. Since SPME allows us to perform all these one action samples, addition advantages, it necessary know whether this...

Bladder cancer (BC) is a common malignancy of the urinary system and leading cause death worldwide. In this work, untargeted metabolomic profiling biological fluids presented as non-invasive tool for bladder biomarker discovery first step towards developing superior methods detection, treatment, prevention well to further our current understanding disease. study, urine samples from 24 healthy volunteers BC patients were subjected using high throughput solid-phase microextraction (SPME) in...

Currently used methods for diagnosing ventilator-associated pneumonia (VAP) are complex, time-consuming and require invasive procedures while empirical antibacterial therapy applies broad spectrum antibiotics that may promote antimicrobial resistance. Hence, novel fast based on alternative markers needed VAP detection differentiation of causative pathogens. Pathogenic bacteria produce a range volatile organic compounds (VOCs), some which potentially serve as biomarkers microorganism...

Introduction Normothermic ex vivo kidney perfusion (NEVKP) is designed to replicate physiological conditions improve graft outcomes. A comparison of the impact hypothermic and normothermic preservation techniques on quality was performed by lipidomic profiling using solid-phase microextraction (SPME) chemical biopsy as a minimally invasive sampling approach. Methods Direct conducted SPME probes coated with mixed-mode extraction phase in porcine autotransplantation model renal donor after...

Liver transplantation is the definitive treatment for end-stage liver failure, but scarcity of donor organs remains a significant challenge. Leveraging from extended criteria donors (ECD) offers potential avenue to address worldwide shortages, though these are more susceptible post-reperfusion injury. This study explores use normothermic ex vivo perfusion (NEVLP) as method organ preservation - an approach that sustains metabolism and facilitates pre-transplant assessments viability via bile...

Abstract The limiting factor in conventional quality assessments of transplanted organs, namely the invasiveness tissue sample collection, has prompted much research on field transplantology to focus development alternative evaluation methods organ quality. In present project, we undertake challenge address need for a new analytical solution graft by using novel metabolomic diagnostic protocol based low‐invasive solid‐phase microextraction. Solid‐phase microextraction probes ca. 0.2 mm...

The development of surgical techniques, immunosuppressive strategies and new organ preservation methods have meant that transplant centers to face the problem an insufficient number organs for transplantation concerning constantly growing demand. Therefore, using from expanded criteria donors developing analytical solutions find parameters or compounds would allow a more efficient assessment quality before are options meeting this challenge. This study proposed bile metabolomic analysis...

Solid organ transplantation is a life-saving intervention for patients suffering from end-stage failure. Although improvements in surgical techniques, standards of care, and immunosuppression have been observed over the last few decades, transplant centers to face problem an insufficient number organs concerning growing demand. An opportunity increase pool intended more frequent use extended criteria development analytical methods allowing better assessment quality minimize risk...

Organ shortage remains a significant challenge in transplantology, prompting efforts to maximize the use of available organs and expand donor pool, including through extended criteria donors (ECDs). However, ECD kidney recipients often face poorer outcomes, higher incidence delayed graft function (DGF), which is linked worse performance, reduced long-term survival, an increased need for interventions like dialysis. This underscores urgent strategies improve early DGF risk assessment optimize...

Kidney transplantation is a life-saving treatment for large number of people with end-stage renal dysfunction worldwide. The procedure associated an increased survival rate and greater quality patient's life when compared to conventional dialysis. Regrettably, transplantology suffers from lack reliable methods organ assessment. Standard diagnostic techniques are limited macroscopic appearance inspection or invasive tissue biopsy, which do not provide comprehensive information about the...

Kidney transplantation is a life-saving treatment for large number of people with end-stage renal dysfunction worldwide. The procedure associated an increased survival rate and greater quality patient's life when compared to conventional dialysis. Regrettably, transplantology suffers from lack reliable methods organ assessment. Standard diagnostic techniques are limited macroscopic appearance inspection or invasive tissue biopsy, which do not provide comprehensive information about the...

Abstract With the ever-increasing shortage of kidney donors, transplant centers are faced with challenge finding ways to maximize their use all available organ resources and extend donor pool, including expanded criteria donors. To address need for a new analytical solution graft quality assessments, we present novel biochemical analysis method based on solid-phase microextraction (SPME) – chemical biopsy. In this study, renal autotransplantation was performed in porcine models simulate two...

<title>Abstract</title> Organ shortage remains a significant challenge in transplantology, prompting efforts to maximize the use of available organs and expand donor pool, including through extended criteria donors (ECD). However, ECD kidney recipients often face poorer outcomes, higher incidence delayed graft function (DGF), which is linked worse performance, reduced long-term survival, increased need for interventions like dialysis. This underscores urgent strategies improve early DGF risk...

<title>Abstract</title> One of the fundamental problems current transplantation is organ shortage. To address this issue, Extended Criteria Donor grafts are utilized. However, requires better preservation strategies and reliable methods assessing graft function. The objective work was to evaluate changes in kidney metabolome intact organs subjected three methods: normothermic ex vivo perfusion (NEVKP), hypothermic machine (HMP) static cold storage (SCS). chemical biopsy used for minimally...