A5044972703- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- RNA modifications and cancer
- Mass Spectrometry Techniques and Applications
- Analytical Methods in Pharmaceuticals
- Hormonal and reproductive studies
- Cytomegalovirus and herpesvirus research
- Metabolism and Genetic Disorders
- Advanced Chemical Sensor Technologies
- Computational Drug Discovery Methods
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Cancer, Hypoxia, and Metabolism
- Nanoplatforms for cancer theranostics
- Advanced Proteomics Techniques and Applications
- Free Radicals and Antioxidants
- Insect Utilization and Effects
- Anesthesia and Sedative Agents
- Drug Transport and Resistance Mechanisms
- Analytical chemistry methods development
- Veterinary Pharmacology and Anesthesia
- Microbial Metabolic Engineering and Bioproduction
- Insect Pest Control Strategies
- Phytoestrogen effects and research
- Dental Trauma and Treatments
Gdańsk Medical University
2013-2024
University of Łódź
2022
Warsaw University of Technology
2022
Silesian University of Technology
2022
Nicolaus Copernicus University
2022
University of Warsaw
2022
Jagiellonian University
2022
Gdańsk University of Technology
2022
University of Opole
2022
University of Gdańsk
2022
Chamomile has been used as an herbal medication since ancient times and is still popular because it contains various bioactive phytochemicals that could provide therapeutic effects. In this study, a simple reliable HPLC method was developed to evaluate the quality consistency of nineteen chamomile samples through establishing chromatographic fingerprint, quantification phenolic compounds determination antioxidant activity. For fingerprint analysis, 12 peaks were selected common similarities...
The discovery of extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, has opened a new frontier in the study signal transduction understanding cell-to-cell communication. EVs play key role regulating various biological processes such as tissue regeneration, blood coagulation, immunomodulation or carcinogenesis. Therefore, they gain much attention potentially therapeutic non-invasive diagnostic targets. In this aspect, comprehensive characterization molecular...
In the present study, development and optimization of a thin film solid phase microextraction method (TF-SPME) was conducted for metabolomics profiling eight steroid compounds (androsterone, dihydrotestosterone, dihydroepiandrosterone, estradiol, hydroxyprogesterone, pregnenolone, progesterone testosterone) from urine samples. For extraction method, two sorbents (PAN-C18 PS-DVB) were used as they are known to be effective isolation low-polarity analytes. The stages sample analyte desorption...
Bladder cancer (BC) is a common malignancy of the urinary system and leading cause death worldwide. In this work, untargeted metabolomic profiling biological fluids presented as non-invasive tool for bladder biomarker discovery first step towards developing superior methods detection, treatment, prevention well to further our current understanding disease. study, urine samples from 24 healthy volunteers BC patients were subjected using high throughput solid-phase microextraction (SPME) in...
Graphene and graphene-based materials have attracted growing interest for potential applications in medicine because of their good biocompatibility, cargo capability possible surface functionalizations.In parallel, prototypic devices been developed to diagnose, imaging track tumor growth cancer patients.There is a number reports on the use graphene its functionalized derivatives design innovative drugs delivery systems, photothermal photodynamic therapy, as platform combine multiple...
Abstract In the postgenome‐sequencing era, several large projects have been running recently. Proteomics and other analysis or structural biology are most active today. Since late 1990s, metabolomics has gaining importance in systems biology, as it provides real‐world end points that complement help interpretation of genomic proteomic data. Comprehensive information about level changes numerous metabolites present analyzed samples is essential metabolomic studies. Therefore, applied...
To evaluate the effects of using ropivacaine combined with dexmedetomidine for sciatic and saphenous nerve blocks in dogs.7 healthy adult Beagles.In phase 1, dogs received each following 3 treatments random order: perineural injections 0.5% (0.4 mL/kg) mixed saline (0.9% NaCl) solution (0.04 mL/kg; DEX0PN), (1 μg/kg; DEX1PN), (2 DEX2PN). In 2, an IV injection diluted DEX1IV). For injections, dose was divided equally between 2 sites. Duration sensory blockade evaluated, plasma concentrations...
In the present study, we developed and validated a fast, simple, sensitive quantitative method for simultaneous determination of eleven nucleosides deoxynucleosides from urine samples. The analyses were performed with use liquid chromatography coupled triple quadrupole mass spectrometry. sample pretreatment procedure was limited to centrifugation, vortex mixing samples methanol/water solution (1:1, v/v), evaporation dissolution steps. analysis lasted 20 min in dynamic multiple reaction...
Aim: The purpose of this work was to develop and validate a rapid robust LC–MS/MS method for the determination dexmedetomidine (DEX) in plasma, suitable analysis large number samples. Method: Systematic approach, Design Experiments, applied optimize ESI source parameters evaluate robustness, therefore, rapid, stable cost-effective assay developed. validated according US FDA guidelines. LLOQ determined at 5 pg/ml. linear over examined concentration range (5–2500 pg/ml), Results: Experimental...
The simultaneous determination of metabolites from biological fluids may provide more accurate information about the current body condition. So far, metabolomics approach has been successfully applied to study mechanism several disorders and search for novel biomarkers. Urine plasma are widely accepted matrices evaluation pathologies, while prostate cancer (CaP) development is still unknown. For this reason, an alternative matrix, seminal fluid, was proposed expand knowledge CaP...
Resistant hypertension (RH) affects about 15-20% of treated hypertensive patients worldwide. RH increases the risk cardiovascular events such as myocardial infarction and stroke by 50%. The pathological mechanisms underlying resistance to treatment are still poorly understood.The main goal this pilot study was determine compare plasma metabolomic profiles in resistant non-resistant patients.We applied untargeted profiling samples collected from 69 subjects with 81 controlled hypertension. To...
In regard to urogenital tract cancer studies, an estimated 340,650 new cases and 58,360 deaths from genital system about 141,140 29330 urinary were projected occur in the United States 2012. The main drawbacks of currently available diagnostic tests constitute low specificity, costliness quite high invasiveness.The goal this pilot study was determine compare urine metabolic fingerprints patients healthy controls.A comparative analysis profile 30 with genitourinary (bladder (n=10), kidney...