A5016309273- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Macrophage Migration Inhibitory Factor
- Immunodeficiency and Autoimmune Disorders
- Reproductive System and Pregnancy
- Systemic Lupus Erythematosus Research
- Bacterial Genetics and Biotechnology
- Abdominal vascular conditions and treatments
- Galectins and Cancer Biology
- Bacillus and Francisella bacterial research
- Cytokine Signaling Pathways and Interactions
- IL-33, ST2, and ILC Pathways
- Digestive system and related health
- Inflammatory Bowel Disease
- interferon and immune responses
- Vibrio bacteria research studies
Harvard University
2012-2015
Beth Israel Deaconess Medical Center
2012-2015
Hadassah Medical Center
2013
Beth Israel Deaconess Hospital
2012-2013
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms
2012
Macrophage migration inhibitory factor (MIF), a proinflammatory cytokine and counterregulator of glucocorticoids, is potential therapeutic target. MIF markedly different from other cytokines because it constitutively expressed, stored in the cytoplasm, present circulation healthy subjects. Thus, concept targeting for intervention challenging need to neutralize ubiquitous protein. In this article, we report that occurs two redox-dependent conformational isoforms. We show one isoforms MIF, is,...
The capacity of pathogens to respond environmental signals, such as iron concentration, is key bacterial survival and establishment a successful infection. Bacillus cereus widely distributed bacterium with distinct pathogenic properties. Hemolysin II (HlyII) one its pore-forming cytotoxins has been shown be involved in pathogenicity number cell animal models. Unlike many other B. factors, HlyII not regulated by pleiotropic transcriptional regulator PlcR but controlled own regulator, HlyIIR....
The costimulatory receptor Slamf6 partially controls lupus-related autoimmunity in congenic Sle1b mice; for instance, the presence of protein isoform Slamf6-H1 Sle1b.Slamf6-H1 mice mitigates disease. Here, we report that young mice, but not or B6 contain a memory T-helper cell subset identified by ∗∗∗[mt]2-fold increase expression 17 genes, chief among which is Spp1, encoding cytokine osteopontin (OPN). These T follicular helper (TFH) cells, including OPN+ TFH expand concomitantly with...
Glucocorticoid-induced TNF receptor family-related protein (GITR) regulates the function of both T cells and antigen-presenting (APCs), while GITR ligand (GITR-L) is largely unknown. Here we evaluate role GITR-L, whose expression restricted to APCs, in development enterocolitis. On injecting naive CD4(+) cells, GITR-L(-/-)Rag(-/-) mice develop a markedly milder colitis than Rag(-/-) mice, which correlates with 50% reduction Ly6C(+)CD11b(+)MHCII(+) macrophages lamina propria mesenteric lymph...
Abstract SLAMF4 is a cell surface receptor, which primarily known as regulator of cytotoxicity and cytokine secretion by mouse NK cells upon engagement with its counter-structure CD48. Here, we found highly expressed on the most mucosal CD8+ T cells, whereas it only some CD44+ splenic cells. Upon transfer naïve SLAMF4− derived from Thy1.2+ B6 mice into Thy1.2− B6, donor were to express after entry mucosa. The increase in SLAMF4+ was due upregulation receptor gene rather than expansion small...
Abstract Glucocorticoid-Induced TNF Receptor family-related protein Ligand (GITR-L) is expressed on antigen presenting cells. Surprisingly, GITR-L−/−Rag−/− mice develop a markedly milder colitis than Rag−/− upon transferring CD4+CD45RBhi T cells or by administering agonistic anti-CD40 antibody. In both models as well in peritonitis model reduced number of Ly6C+CD11b+MHCII+ macrophages was found the inflamed sites GITR-L−/− mice. By contrast, non-differentiated monocytes approximately...
Abstract Patients with X-linked lymphoproliferative disease (XLP) who lack functional SLAM-associated protein (SAP) have both impaired NKT cells development and progressive agammaglobulinemia. We report that α-galactosylceramide (αGalCer) activated positively regulate antibody responses to haptenated antigens at multiple checkpoints, including germinal center formation affinity maturation. cell-dependent B cell were absent in SAP-/-.B6 mice cells. However, the small number of SAP-deficient...
Abstract A major role for the Slamf6 gene in pathogenesis of lupus-related autoimmunity has been revealed by analysis a number congenic mutant mouse strains. Importantly, upon introduction one copy BAC transgene expressing protein isoform Slamf6-H1 markedly ameliorates T cell-dependent lupus Sle1b mouse, which lacks H1. Here we report that PD-1+ subset memory CD4+ cells dramatically expands mice, but not express Slamf6-H1. Further showed this population mainly consists CXCR5+ follicular...
Abstract Signaling lymphocyte activation molecule (Slamf1) is a T cell co-stimulatory and regulator of cytokine production by macrophages dendritic cells. Because Slamf1 positively regulates microbicidal mechanisms in macrophages, we evaluate whether would affect enterocolitis. To induce colitis, mouse CD45RBhi CD4+ cells were transferred into RAG-/- or Slamf1-/- recipient mice. In second set experiments, an agonistic αCD40 antibody was administered to the same strains colitis. Whilst...