A5015617895- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Food Allergy and Anaphylaxis Research
- Immune Cell Function and Interaction
- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Mast cells and histamine
- Immune Response and Inflammation
- HER2/EGFR in Cancer Research
- Immunodeficiency and Autoimmune Disorders
- vaccines and immunoinformatics approaches
- Contact Dermatitis and Allergies
- Cell Adhesion Molecules Research
- Protein purification and stability
- Cancer Immunotherapy and Biomarkers
- Hepatitis B Virus Studies
- Urticaria and Related Conditions
- CAR-T cell therapy research
- Galectins and Cancer Biology
- Cancer Genomics and Diagnostics
- Diabetes and associated disorders
- Radiopharmaceutical Chemistry and Applications
Agency for Quality Assurance and Accreditation Austria
2018
Baxter (Austria)
2006-2015
F-star (United Kingdom)
2015
Forschungs- und Beratungsstelle Arbeitswelt
1993-2010
University of Nottingham
2006
Medical University of Vienna
2006
Novartis (Austria)
1997-2005
Ingenium (Germany)
2003-2005
University Hospital Augsburg
2004
Novartis (Switzerland)
1998-2000
Cutaneous lymphocyte antigen (CLA), defined by the HECA-452 antibody, is a cell surface glycoprotein found on subset of T cells in peripheral blood that binds specifically to E-selectin. This marker present majority at sites cutaneous inflammation and immune responses. Based upon such evidence, an association between CLA expression skin homing has been proposed. To understand better this relationship, we asked whether putative disease-related, antigen-specific expressed CLA. In study,...
Yeast surface display libraries of human IgG1 Fc regions were prepared in which loop sequences at the C-terminal tip CH3 domain randomized. A high percentage these library members bound to soluble CD64 and Protein indicating that randomization step did not grossly interfere with overall structure displayed Fc. Sorting by FACS for binders against HER2/neu yielded antigen-specific (Fcab; antigen binding) one was affinity matured, resulting Fcab clone H10-03-6 showed >10-fold improvement...
Abstract Recent studies have demonstrated that the reduction of core fucosylation on N ‐glycans human IgGs is responsible for a clearly enhanced antibody‐dependent cellular cytotoxicity (ADCC). This finding might give access to improved active therapeutic antibodies. Here, expression tumor antigen‐specific antibody IGN311 was performed in glyco‐optimized strain moss Physcomitrella patens. Removal plant specific ‐glycan structures this host achieved by targeted knockout corresponding genes...
Macrophage migration inhibitory factor (MIF), a proinflammatory cytokine and counterregulator of glucocorticoids, is potential therapeutic target. MIF markedly different from other cytokines because it constitutively expressed, stored in the cytoplasm, present circulation healthy subjects. Thus, concept targeting for intervention challenging need to neutralize ubiquitous protein. In this article, we report that occurs two redox-dependent conformational isoforms. We show one isoforms MIF, is,...
The aim of the present study was to produce glycosylation variants therapeutic Lewis Y-specific humanized IgG1 antibody IGN311 enhance cell-killing effector function. This achieved via genetic engineering machinery antibody-producing host. Antibody genes were transiently cotransfected with acetyl-glycosaminyltransferase-III into human embryonic kidney-EBV nuclear antigen cells. A control wild-type antibody, IGN311wt, expressed in same host using identical expression vectors, but without...
Abstract The majority of cancer cells derived from epithelial tissue express Lewis-Y (LeY) type difucosylated oligosaccharides on their plasma membrane. This results in the modification cell surface receptors by LeY antigen. We used epidermal growth factor (EGF) receptor family members ErbB1 and ErbB2 as model systems to investigate whether sugar moiety can be exploited block signaling human tumor (i.e., SKBR-3 A431, a breast vulval carcinoma, respectively). monoclonal anti-LeY antibody...
FS102 is a HER2-specific Fcab (Fc fragment with antigen binding), which binds HER2 high affinity and recognizes an epitope that does not overlap those of trastuzumab or pertuzumab. In tumor cells express levels HER2, caused profound internalization degradation leading to cell apoptosis. The antitumor effect in patient-derived xenografts (PDXs) correlated strongly the amplification status tumors. Superior activity over combination pertuzumab was observed vitro vivo when gene copy number equal...
We investigated the longevity of allergen-specific Th cells derived from patients suffering either allergic rhinitis or atopic dermatitis. T cell clones (TCC) specific for seasonal and perennial allergens were raised. To determine whether these TCC long-lived in vivo, PBMC polyclonal lines, collected established inside a period up to 4 years, screened interest. For this purpose, tracing protocol was which oligonucleotides TCR beta-chain hypervariable junctional region used as tools identify...
A prospective study of the immune response after hepatitis B vaccination was carried out in 32 insulin dependent diabetes mellitus (IDDM) patients and their age sex matched healthy controls. sensitive, immunoenzymatic technique used, able to detect vitro specific antibody production by mitogen stimulated individual cells. In-vivo serologic with a standard scheme (0, 1 6 months) 20 micrograms recombinant (HB) vaccine significantly impaired IDDM both respect number nonresponders (25 versus 3%,...
The blood group-related Lewis Y antigen is expressed on the majority of human cancers epithelial origin with only limited expression normal tissue. Therefore, represents an interesting candidate for antibody-based treatment strategies. Previous experiments showed that humanized Y-specific monoclonal antibody, IGN311, reduced ErbB-receptor-mediated stimulation mitogen-activated protein kinase by altering receptor recycling. Here, we tested whether binding IGN311 to growth factor receptors...