A5052310057- Gut microbiota and health
- Probiotics and Fermented Foods
- Listeria monocytogenes in Food Safety
- Clostridium difficile and Clostridium perfringens research
- Bacillus and Francisella bacterial research
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Microbial Inactivation Methods
- Microbial Metabolites in Food Biotechnology
- Identification and Quantification in Food
- Essential Oils and Antimicrobial Activity
- Food Safety and Hygiene
- Digestive system and related health
- Mass Spectrometry Techniques and Applications
- Gastrointestinal motility and disorders
- Yersinia bacterium, plague, ectoparasites research
- Bacterial Genetics and Biotechnology
- Chemical Synthesis and Analysis
- Biopolymer Synthesis and Applications
- Food Supply Chain Traceability
- Medical Research and Treatments
- Vibrio bacteria research studies
- Molecular Biology Techniques and Applications
- Salmonella and Campylobacter epidemiology
- Enterobacteriaceae and Cronobacter Research
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2010-2024
Agence Nationale des Fréquences
2007-2021
Sorbonne Université
2015
Virologie et Immunologie Moléculaires
2007-2009
Institut National de la Recherche Agronomique
2005
In humans, the intestinal microbiota plays an important role in maintenance of host health by providing energy, nutrients, and immunological protection. Applying current molecular methods is necessary to surmount limitations classical culturing techniques order obtain accurate description composition.Here we report on comparative assessment human fecal from three age-groups: infants, adults elderly. We demonstrate that undergoes maturation birth adulthood further altered with ageing. The...
The intestinal microbiota is suspected to play a role in colitis and particularly inflammatory bowel disease (IBD) pathogenesis. aim was compare the fecal composition of patients with that healthy subjects (HS).fecal samples from 22 active Crohn's (A-CD) patients, 10 CD remission (R-CD), 13 ulcerative (A-UC) 4 UC (R-UC), 8 infectious (IC) 27 HS were analyzed by quantitative real-time polymerase chain reaction (PCR) targeting 16S rRNA gene. Bacterial counts transformed logarithms (Log(10)...
Pollution of the environment by human and animal faecal pollution affects safety shellfish, drinking water recreational beaches. To pinpoint origin contaminations, it is essential to define differences between microbiota that farm animals. A strategy based on real-time quantitative PCR (qPCR) assays was therefore developed applied compare composition intestinal these two groups. Primers were designed quantify 16S rRNA gene from dominant subdominant bacterial TaqMan® probes defined for qPCR...
To date, there is significant controversy as to the survival of yogurt bacteria (namely, Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) after passage through human gastrointestinal tract. Survival both bacterial species in feces was investigated by culture on selective media. Out 39 samples recovered from 13 healthy subjects over a 12-day period fresh intake, 32 37 contained viable S. (median value 6.3 x 10(4) CFU g(-1) feces) L. 7.2 10(4)CFU feces),...
Clostridium perfringens is both an ubiquitous environmental bacterium and the fourth most common causative agent of foodborne outbreaks (FBOs) in France Europe. These are known to be caused by C. enterotoxin (CPE) encoded cpe gene. However, additional information on toxin/virulence gene content has become available last few years. Therefore, understand enteropathogenicity this bacterium, we need describe toxin virulence genes strains involved FBOs. In study, used a new real-time PCR typing...
Bacillus thuringiensis (Bt) belongs to the cereus (Bc) group, well known as an etiological agent of foodborne outbreaks (FBOs). Bt distinguishes itself from other Bc by its ability synthesize insecticidal crystals. However, search for these crystals is not routinely performed in food safety or clinical investigation, and actual involvement occurrence FBOs known. In present study, we reveal that was detected context 49 declared France between 2007 2017. 19 FBOs, only microorganism detected,...
Bacillus cereus (Bc) is a wide group of Gram-positive and spore-forming bacteria, known to be the etiological agents various human infections, primarily food poisoning. The Bc includes enteropathogenic strains able germinate in digestive tract produce enterotoxins such as Nhe, Hbl, CytK. One species group, thuringiensis (Bt), has unique feature producing insecticidal crystals during sporulation, making it an important alternative chemical pesticides protect crops from insect pest larvae....
Bacillus cereus sensu lato (Bcsl) is a group of closely related bacterial species known for their resistant spores, enabling them to persist in dormant state and thereby colonize adapt across diverse environments. Bcsl its harmful impact on human health, producing toxins that cause emetic diarrheal syndromes or provoking opportunistic infections hospitals. Importantly, the most frequent confirmed presumptive causative agent associated with foodborne outbreaks (FBOs) France. In our study, we...
The aim of this study was to evaluate the survival <i>Lactobacillus rhamnosus</i> R11 and acidophilus</i> R52 in human digestive tract their effects on microbiota homeostasis. We designed an open trial including 14 healthy volunteers. A 3-week exclusion period fermented products followed by a 12-day consumption 4 capsules daily containing 2 × 10<sup>9</sup><i>L. 1 10<sup>8</sup><i>L. R52, wash-out period. strains dominant bacterial groups...
Foodborne illnesses are syndromes including nausea, vomiting, abdominal cramps and diarrhea caused by the ingestion of food contaminated chemical substances or microorganisms and/or their toxins. Reporting poisoning has been mandatory for European Union member states since 2003. Data collected Food Safety Authority (EFSA) include number outbreaks per causative agent, human cases as well hospitalization death rates. This paper will focus on meat products vehicles FBOs. Following legal...
Enterococci are natural inhabitants of the human gastrointestinal tract and main Gram-positive facultative anaerobic cocci recovered in faeces. They also present a variety fermented dairy meat products, some rare isolates responsible for severe infections such as endocarditis meningitis. The aim study was to evaluate effect Camembert cheese consumption by healthy volunteers on faecal enterococcal population. A highly specific real-time quantitative PCR approach designed used type species Two...
Abstract Bacillus cereus is responsible for foodborne outbreaks worldwide. Among the produced toxins, cereulide induces nausea and vomiting after 30 min to 6 h following consumption of contaminated foods. Cereulide, a cyclodepsipeptide, an ionophore selective K + in solution. In electrospray, selectivity reduced as [M Li] ; Na] NH 4 ] can also be detected without adding corresponding salts. Two forms are possible alkali‐cationized ions: charge‐solvated (CS) that exclusively dissociates by...
A fast and simple method for whole-cell hybridization using fluorescently labeled rRNA-targeted peptide nucleic acid (PNA) probes was developed use in detection identification of thermophillic lactobacilli cells growing milk or present industrial starter cultures.The protocol uses a filtration technique the samples epifluorescence microscopy as system, is completed within 1.5 h.Seven oligonucleotide with different ranges specificity have been tested situ experiments against number collection...
Although relative air humidity (RH) strongly influences microbial survival, its use for fighting surface pathogens in the food industry has been inadequately considered. We asked whether RH control could destroy Listeria monocytogenes EGDe by envelope damage. The impact of dehydration phosphate-buffered saline (PBS) at 75%, 68%, 43% and 11% on bacterial was investigated using flow cytometry atomic force microscopy. Changes after rehydration protein secondary structure peptidoglycan were...
Bacillus thuringiensis (Bt), belonging to the cereus (Bc) group, is commonly used as a biopesticide worldwide due its ability produce insecticidal crystals during sporulation. The use of Bt, especially subspecies aizawai and kurstaki, control pests such Lepidoptera, generally involves spraying mixtures containing spores on crops intended for human consumption. Recent studies have suggested that consumption commercial Bt strains may be responsible foodborne outbreaks (FBOs). However, genetic...
Listeria monocytogenes (L. monocytogenes) is an ubiquitous bacterium that causes a severe foodborne illness. It established the contamination of food production facilities over long time period, are potentially one major product sources. L. persistence was observed in almost all sectors and particularly pork facilities. The characterization such therefore crucial to improve safety prevent outbreaks. These strains called persistent but this trait remains loosely defined, no genetic...