A5000056147- Enzyme Catalysis and Immobilization
- Microbial bioremediation and biosurfactants
- Amino Acid Enzymes and Metabolism
- Biochemical Acid Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Cyclopropane Reaction Mechanisms
- Biomedical Text Mining and Ontologies
- Biochemical and biochemical processes
- Enzyme Structure and Function
- Pharmacogenetics and Drug Metabolism
- Metalloenzymes and iron-sulfur proteins
- Vanadium and Halogenation Chemistry
- Surfactants and Colloidal Systems
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Research Data Management Practices
- Free Radicals and Antioxidants
- Scientific Computing and Data Management
- Enzyme Production and Characterization
- Plant biochemistry and biosynthesis
Vidyasirimedhi Institute of Science and Technology
2020-2024
Although flavin-dependent halogenases (FDHs) are attractive biocatalysts, their practical applications limited because of low catalytic efficiency. Here, we investigated the reaction mechanisms and structures tryptophan 6-halogenase (Thal) from Streptomyces albogriseolus using stopped-flow, rapid-quench flow, quantum/mechanics molecular mechanics calculations, crystallography, detection intermediate (hypohalous acid [HOX]) liberation. We found that key flavin intermediate,...
Abstract Enzymatic electrophilic halogenation is a mild tool for functionalization of diverse organic compounds. Only few groups native halogenases are capable catalyzing such reaction. In this study, we used mechanism‐guided strategy to discover the activity catalyzed by non‐native halogenases. As ability form hypohalous acid (HOX) key halogenation, flavin‐dependent monooxygenases/oxidases forming C4a‐hydroperoxyflavin (Fl C4a‐OOH ), as dehalogenase, hydroxylases, luciferase and...
Anthraquinone-fused enediynes are anticancer natural products featuring a DNA-intercalating anthraquinone moiety. Despite recent insights into anthraquinone-fused enediyne (AQE) biosynthesis, the enzymatic steps involved in biogenesis remain to be elucidated. Through combination of
Abstract Enzymatic electrophilic halogenation is a mild tool for functionalization of diverse organic compounds. Only few groups native halogenases are capable catalyzing such reaction. In this study, we used mechanism‐guided strategy to discover the activity catalyzed by non‐native halogenases. As ability form hypohalous acid (HOX) key halogenation, flavin‐dependent monooxygenases/oxidases forming C4a‐hydroperoxyflavin (Fl C4a‐OOH ), as dehalogenase, hydroxylases, luciferase and...