A5057516915- Microbial Natural Products and Biosynthesis
- Natural product bioactivities and synthesis
- Biological Activity of Diterpenoids and Biflavonoids
- Plant biochemistry and biosynthesis
- Marine Sponges and Natural Products
- Phytochemistry and Biological Activities
- Chemical synthesis and alkaloids
- Sesquiterpenes and Asteraceae Studies
- Genomics and Phylogenetic Studies
- Phytochemical compounds biological activities
- Bioactive Compounds and Antitumor Agents
- Synthetic Organic Chemistry Methods
- Gut microbiota and health
- Plant Toxicity and Pharmacological Properties
- Fungal Biology and Applications
- Natural Compounds in Disease Treatment
- Carbohydrate Chemistry and Synthesis
- Ginkgo biloba and Cashew Applications
- Plant Pathogens and Fungal Diseases
- Cholinesterase and Neurodegenerative Diseases
- Phenothiazines and Benzothiazines Synthesis and Activities
- Neurological Disease Mechanisms and Treatments
- Traditional and Medicinal Uses of Annonaceae
- Synthesis and Biological Activity
- Advanced biosensing and bioanalysis techniques
Nanyang Technological University
2019-2024
Zhejiang University
2023-2024
Fudan University
2013-2024
National Research Centre
2024
Minhang District Central Hospital
2020
Peking University
2020
Lishui Academy of Agricultural Sciences
2015
Naturally occurring hydrazones are rare despite the ubiquitous usage of synthetic in preparation organic compounds and functional materials. In this study, we discovered a family novel microbial metabolites (tasikamides) that share unique cyclic pentapeptide scaffold. Surprisingly, tasikamides A–C (1–3) contain hydrazone group (C═N─N) joins peptide scaffold to an alkyl 5-hydroxylanthranilate (AHA) moiety. We biosynthesis 1–3 requires two discrete gene clusters, with one encoding nonribosomal...
Ten new lycodine-type alkaloids, named casuarinines A–J (1–10), along with eight known analogues (11–18), were isolated from the whole plant of Lycopodiastrum casuarinoides. The structures established by spectroscopic methods and chemical transformations. Casuarinines A–D (1–4) J (10) are common lycodine alkaloids possessing four connected six-membered rings, while tricyclic E–H (5–8) piperidine ring cleavage products. In particular, casuarinine I (9) has an unprecedented five-membered...
Nine unexpected new flavonol glycoside cyclodimers in the truxinate (1-7, biginkgosides A-G, respectively) or truxillate [biginkgosides H (8) and I (9)] forms were isolated as minor components from extract of Ginkgo biloba leaves. The dimers possess an unusual cyclobutane ring formed by a [2+2]-cycloaddition between two symmetric (for compounds 1-5 7-9) nonsymmetric 6) coumaroyl glucorhamnosides. A plausible biosynthetic pathway for these based on frontier molecular orbital theory...
A preliminary phytochemical investigation on the MeOH extract of leaves and twigs endangered ornamental plant Michelia shiluensis led to isolation 16 sesquiterpenoids. The isolated compounds comprised germacrane- (1–4, 13, 14), guaiane- (5–9, 15), amorphane- (10), eudesmane-type (11, 12, 16) new structures (1–12) were elucidated by spectroscopic computational methods, their absolute configurations (except for 9) assigned single-crystal X-ray diffraction crystallographic data and/or...
We report the genome-guided discovery of sungeidines, a class microbial secondary metabolites with unique structural features. Despite evolutionary relationships dynemicin-type enediynes, sungeidines are produced by biosynthetic gene cluster (BGC) that exhibits distinct differences from known enediyne BGCs. Our studies suggest assembled two octaketide chains processed differently than those enediynes. The biosynthesis also involves activating sulfotransferase promotes dehydration reaction....
Seven [4 + 2]-type triterpene-diterpene hybrids derived from a rearranged or normal lanostane unit (dienophile) and an abietane moiety (diene), forrestiacids E-K (1-7, respectively), were further isolated characterized Pseudotsuga forrestii (a vulnerable conifer endemic to China). The intriguing molecules revealed with the guidance of LC-MS/MS-based molecular ion networking strategy combined conventional phytochemical procedures. Their chemical structures absolute configurations established...
Seven new naturally occurring barrigenol-like compounds, camellianols A–G (1–7), and 10 known triterpenoids were isolated from the twigs leaves of cultivated endangered ornamental plant Camellia crapnelliana. According to ECD octant rule for saturated cyclohexanones, absolute configurations D (4) E (5) defined. The backbones remaining isolates are assumed have same configuration as compounds 4, 5, harpullone (12). Compounds 2, 3, 9, 10, 13, 16 exhibited inhibitory effects on protein tyrosine...
Anthraquinone-fused enediynes (AQEs) are renowned for their distinctive molecular architecture, reactive enediyne warhead, and potent anticancer activity. Although the first members of AQEs, i.e., dynemicins, were discovered three decades ago, how nitrogen-containing carbon skeleton is synthesized by microbial producers remains largely a mystery. In this study, we showed that recently sungeidine pathway "degenerative" AQE contains upstream enzymes biosynthesis. Retrofitting with genes from...
Produced by a newly isolated Streptomycetes strain, meijiemycin is gigantic linear polyene-polyol that exhibits structural features not seen in other members of the family. We propose biosynthetic mechanism and demonstrate inhibits hyphal growth inducing aggregation ergosterol restructuring fungal plasma membrane.
We present the newly isolated Streptomyces sungeiensis SD3 strain as a promising microbial chassis for heterologous production of secondary metabolites. S. exhibits several advantageous traits chassis, including genetic tractability, rapid growth, susceptibility to antibiotics, and metabolic capability supporting metabolism. Genomic transcriptomic sequencing unveiled primary capabilities biosynthetic pathways SD3, previously unknown pathway responsible biosynthesis streptazone B1. The unique...
Anthraquinone-fused enediynes are anticancer natural products featuring a DNA-intercalating anthraquinone moiety. Despite recent insights into anthraquinone-fused enediyne (AQE) biosynthesis, the enzymatic steps involved in biogenesis remain to be elucidated. Through combination of
The human gastrointestinal microbiota plays a vital role in maintaining host health and preventing diseases, prompting the creation of simulators to replicate this intricate system. Simulator Human Intestinal Microbial Ecosystem (SHIME®), multicompartment dynamic simulator, has emerged as pivotal vitro model for studying interactions interferences within gut microbiota. continuous real-time monitoring hallmarks, along with programmatically flexible setup, bestow SHIME® mimicking entire...
Ten diterpenoids including three new abietanes (1-3) were isolated from the twigs and needles of Podocarpus imbricatus, an endangered conifer growing in a Cantonese garden. The structures established by means spectroscopic methods. Among isolates, 3β-hydroxy-abieta-8,11,13-trien-7-one (5), decandrin G (6), 7,15-pimaradien-18-oic acid (8) showed significant anti-neuroinflammatory activities inhibiting overproduction nitric oxide (NO) lipopolysaccharide (LPS)-stimulated murine BV-2 microglial...
Abstract Four novel naturally occurring diastereoisomers of dinor‐eudesmenes, named chloranthones A–D ( 1 – 4 , resp.), were isolated as minor components from the EtOH extract aerial parts Chloranthus elatior. The unprecedented framework was established using extensive 2D‐NMR techniques. Their absolute configurations deduced observed Cotton effects in their circular dichroism (CD) spectra. A plausible biosynthetic pathway to dinor‐eudesmenes is proposed.