A5061039656- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Cardiovascular Disease and Adiposity
- Mitochondrial Function and Pathology
- Apelin-related biomedical research
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Advanced Proteomics Techniques and Applications
- Cardiovascular Function and Risk Factors
- Lipid metabolism and biosynthesis
- Nitric Oxide and Endothelin Effects
- Pancreatic function and diabetes
- Heat shock proteins research
- Autophagy in Disease and Therapy
- Mass Spectrometry Techniques and Applications
- Cardiac, Anesthesia and Surgical Outcomes
- Hormonal Regulation and Hypertension
- Connexins and lens biology
- Cardiac Ischemia and Reperfusion
- Metabolism and Genetic Disorders
- Calpain Protease Function and Regulation
- Machine Learning in Bioinformatics
- Signaling Pathways in Disease
- Circular RNAs in diseases
- Lipid metabolism and disorders
- Heme Oxygenase-1 and Carbon Monoxide
TransCanada (Canada)
2021
Université de Montréal
2015-2017
Montreal Heart Institute
2015-2017
University of Toronto
2008-2014
Toronto Public Health
2011-2014
Heart and Stroke Foundation
2008-2013
McGill University
2001-2009
University Health Network
2009
GlaxoSmithKline (United Kingdom)
2009
Montreal General Hospital
2001-2009
α-Crystallin B (cryAB) is the most abundant small heat shock protein in cardiomyocytes (CMs) and has been shown to have potent antiapoptotic properties. Because mechanism by which cryAB prevents apoptosis not fully characterized, we examined its protective effects at cellular level silencing mouse neonatal CMs using lentivector-mediated transduction of short hairpin RNAs. Subcellular fractionation whole hearts showed that cytosolic under control conditions, after H(2)O(2) exposure, it...
In‐depth proteomic analyses offer a systematic way to investigate protein alterations in disease and, as such, can be powerful tool for the identification of novel biomarkers. Here, we analyzed data from transgenic mouse model with cardiac‐specific overexpression activated calcineurin ( C n A ), which results severe cardiac hypertrophy. We applied statistically filtering and false discovery rate correction methods identify 52 proteins that were significantly different hearts compared...
Cardiac-specific overexpression of a constitutively active form calcineurin A (CNA) leads directly to cardiac hypertrophy in the CNA mouse model. Because is prominent characteristic many cardiomyopathies, we deduced that delineating proteomic profile ventricular tissue from this model might identify novel, widely applicable therapeutic targets. Proteomic analysis was carried out by subjecting fractionated samples mice and their WT littermates gel-free liquid chromatography linked shotgun...
Abstract TLRs are essential mediators of host defense against infection via recognition unique microbial structures. Recent observations indicate that TLR4, the principal receptor for bacterial LPS, may also be activated by noninfectious stimuli including host-derived molecules and environmental oxidant stress. In mice, susceptibility to ozone-induced lung permeability has been linked wild-type allele whereas deficiency TLR4 predisposes lethal injury in hyperoxia. To precisely characterize...
Recent advances in mass spectrometry and bioinformatics have provided the means to characterize complex protein landscapes from a wide variety of organisms cell types. Development standard proteomes exhibiting all proteins involved normal physiology will facilitate delineation disease mechanisms. Here, we examine wild-type cardiac proteome using data obtained subcellular fractionation protocol combination with multidimensional identification proteomics approach. We identified 4906 which were...
Calnexin (CNX) is an endoplasmic reticulum (ER) quality control chaperone that has been implicated in ER stress. stress a prominent pathological feature of various pathologic conditions, including cardiovascular diseases. However, the role CNX and not studied heart. In present study, we aimed to characterize cardiomyocyte physiology with respect stress, apoptosis, Ca(2+) cycling. We demonstrated significantly decreased mRNA protein levels by LentiVector mediated transduction targeting...
Background Endoplasmic reticulum ( ER ) resident protein 44 p44) is a member of the disulfide isomerase family, induced during stress, and may be involved in regulating Ca 2+ homeostasis. However, role p44 cardiac development function unknown. The aim this study was to investigate mice, zebrafish, embryonic stem cell ESC )‐derived cardiomyocytes determine underlying p44. Methods Results We generated characterized −/− morphant zebrafish embryos, ‐derived cardiomyocytes. Deletion mouse caused...
An integrative bioinformatic and experimental approach to elucidate potential miRNA targets for further study validation. The incorporation of multiple data sources can help address the high false positive rate target predictions.
Diabetic hearts exhibit intracellular lipid accumulation. This suggests that the degree of fatty acid oxidation (FAO) in these is insufficient to handle elevated uptake. We previously showed palmitate impaired rate FAO primary rat neonatal cardiomyocytes. Here we were interested characterizing site impairment induced by since it may shed light on metabolic dysfunction leads accumulation diabetic hearts.We measured oxidation, acetyl-CoA and carnitine palmitoyl transferase (Cpt1b) activity....
Abstract Abstract: Introduction: Diabetes is a major contributor to cardiovascular disease. There growing body of evidence pointing towards intra-myocellular lipid accumulation as an integral etiological factor. Here we aimed determine the effect two common fatty acids on and cellular stress in primary cardiomyocytes. Methods: We evaluated biochemically (by triacylglyceride assay radiolabeled acid uptake assay) well histologically BODIPY 493/503 staining) mouse rat neonatal cardiomyocytes...
Expression of the vasoactive peptide Urotensin II (UII) is elevated in a number cardiovascular diseases.Here, we sought to determine effect UII receptor (UT) gene deletion mouse model atherosclerosis.UT knockout (KO) mice were crossed with ApoE KO generate UT/ApoE double (DKO) mice. Mice placed on high-fat Western-type diet for 12 weeks. We evaluated degree atherosclerosis and hepatic steatosis by histology. In addition, serum glucose, insulin, lipids determined. DKO exhibited significantly...
Cells contain a large yet, constant genome, which contains all the coding information necessary to sustain cellular physiology. However, proteins are end products of genes, and hence dictate phenotype cells tissues. Therefore, proteomics can provide key for elucidation physiological pathophysiological mechanisms by identifying protein profile from The relatively novel techniques used study thus have potential improve diagnostic, prognostic, as well therapeutic avenues. In this review, we...