A5060730463- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Veterinary Oncology Research
- Acute Myeloid Leukemia Research
- T-cell and B-cell Immunology
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Mycobacterium research and diagnosis
- Veterinary medicine and infectious diseases
- Diphtheria, Corynebacterium, and Tetanus
- HIV Research and Treatment
- Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Viral Infections and Vectors
- Neuroscience of respiration and sleep
- Neonatal and fetal brain pathology
- Erythrocyte Function and Pathophysiology
- Genomic variations and chromosomal abnormalities
- Animal Genetics and Reproduction
- Vector-borne infectious diseases
- Neonatal Respiratory Health Research
- Bladder and Urothelial Cancer Treatments
The University of Tokyo
2021-2025
Obihiro University of Agriculture and Veterinary Medicine
2015-2025
Veterinary Medical Center
2021
Gifu University
2019-2020
A homozygous individual for ITGB7 gene mutation, an autosomal recessive congenital disorder in Holstein cattle, was retrospectively identified by genotyping of 195 stored blood from patients less than 12 months age. Other 24 (12.3%) showed heterozygous. The a 107-day-old female calf born on March 2017, who presented with chronic diarrhea and severe hypocholesterolemia suggesting hereditary cholesterol deficiency (CD), but analysis negative CD. patient watery diarrhea, dehydration, extreme...
Bovine leukemia virus (BLV), a retrovirus that is widespread worldwide, causes enzootic bovine leukosis (EBL), B-cell leukemia/lymphoma with poor prognosis ultimately results in death. In Japan, the number of cattle infected this increasing, and it estimated more than 35% are currently infected. Since no vaccines or treatments against BLV infection available, important to establish method early diagnosis for EBL reduce economic losses caused by disposal where large expensive beef raised. We...
This study investigated the seroprevalence of bovine leukemia virus (BLV) in beef breeding cattle Ibaraki, revealing a 43.0% with bimodal distribution farms classified as either BLV-negative or very-highly infected. These results underscore need to implement appropriate strategies for infected farms. In particular, determining culling prioritization is absolutely necessary. It was revealed that effective control difficult based on types operation herd size. On other hand,...
Circulating tumor DNA (ctDNA), which carries tumor-specific mutations, is an emerging candidate biomarker for malignancies and monitoring disease status in various human tumors. Recently, BRAF V595E mutation has been reported 80% of dogs with urothelial carcinoma. This study investigates the allele concentration circulating cell-free (cfDNA) assesses clinical significance BRAF-mutated ctDNA levels canines A total 15 carcinoma were included. cfDNA was measured using a real-time polymerase...
Enzootic bovine leukosis (EBL) is one of neoplasms caused by leukemia virus (BLV). Although EBL typically observed in cattle over 3 years old, several cases onset under old have been reported Japan. The mechanism for young remains unclear. genetic variation BLV limited, the variations could affect viral properties relating to pathogenesis. purpose this study was clarify relationship between early and groups. Moreover, we also aimed characterize that cause EBL. Whole genome sequences 72 50...
We performed a clonality analysis using polymerase chain reaction (PCR) for immunoglobulin heavy (IgH) gene rearrangement, specifically with regard to its utility as method diagnose bovine B-cell lymphoma. PCR IgH rearrangement indicated monoclonal proliferation of B-cells in 24 35 cattle In contrast, lymph nodes and tumor tissues from 65 diagnosed tumors other than lymphoma non-tumors revealed polyclonal population B-cells. Sensitivity, specificity, positive predictive value, negative value...
Enzootic bovine leukosis (EBL) is typically observed in cattle older than 3 years, but some cases of onset younger years have been reported Japan. BoLA-DRB3 polymorphisms are associated with susceptibility to EBL onset. However, little known about the relationship between and young cattle. In present study, we performed genotyping 59 (25 Holstein-Friesian 34 Japanese Black) compared results those 69 (38 31 Black). The BoLA-DRB3*15:01 allele was detected at a frequency 37.3% (48.0% 29.4%...
A 21-mo-old Japanese Black beef cow had swollen mandibular and superficial cervical lymph nodes. Fine-needle aspiration cytology of the node revealed large lymphoblasts with mitoses present. The bovine leukemia virus (BLV) proviral load was relatively high, phylogenetic analysis whole BLV genome classified strain as one high viral replication activity. Genotyping leukocyte antigen genes indicated that susceptible to enzootic leukosis (EBL). bone morphogenetic protein 6 ( BMP6) gene promoter...
A 19-mo-old Holstein heifer was inactive and dyspneic. Physical examination revealed wheezing, exophthalmos, a cervical mass, lymphadenopathy. Cytology of the mass lymph nodes showed predominantly large atypical lymphocytes. Lactate dehydrogenase thymidine kinase activities were elevated. Although nested PCR for bovine leukemia virus (BLV) using blood positive, quantitative low number provirus copies. Autopsy enlargement most examined, as well white masses various sizes in muscles left...
A serological survey of Borrelia infection dogs was performed in Sapporo, Japan, where garinii detected 2011. total 314 serum samples were collected from that visited three animal hospitals Sapporo 2012 to 2014. The two-step evaluation method, involving screening ELISA followed by Western blot analysis, used detect antibodies against species. 34 positive ELISA. Among those samples, 32 for spp. blot. These findings suggest the (10.2%) generated burgdorferi sensu lato, such as B. or afzelii....
A 23-month-old Holstein-Friesian heifer presented with inactivity and diarrhea. On physical examination, no enlargement of superficial lymph nodes was observed. Hematological examination revealed lymphocytosis. The bovine leukemia virus (BLV) proviral load 2,122 copies/10 ng DNA, BLV classified as Group C based on whole genome phylogenetic analysis. Monoclonal proliferation B-cells monoclonal integration the provirus in were detected by a clonality test inverse PCR, respectively. Although...
Peripheral B-lymphocyte clonality of 274 bovine leukemia virus-infected cattle with lymphocytosis was analyzed using PCR based on sequences the variable region immunoglobulin H chain. None showed monoclonal proliferation, while 10, 31, and 233 minor-clonal, oligoclonal, polyclonal respectively. A total 163 were analyzable following year, maintained in 157, indicating persistent (PL). 157 PL minor-clonal 6 (3.8%), oligoclonal 8 (5.1%), 143 (91.1%). higher rate enzootic leukosis (EBL) onset...
Three Shiba goats aged 1 to 7 years kept in Ibaraki prefecture Japan were presented with chief complaint of lumbar paralysis or gait abnormalities. As cerebrospinal setariasis suspected all cases at the first stage, ivermectin was administered treat, but response insufficient. Necropsy revealed abscess formation on ventral side spine T5 Case 1, T5-6 2, and C7-T1 3, causing compression spinal cord three cases. In addition setariasis, vertebral should be considered as a cause paresis...
A 5-year-old Japanese Black cow presented with astasia. Bovine leukemia virus (BLV) was detected in the peripheral blood lower proviral load (PVL). No enlargement of surface lymph nodes or lymphocytosis observed. Necropsy revealed no enlarged thoracic, abdominal, pelvic cavity. Spinal epidural and peri-medullary adipose tissue increased spinal canal lumbar to sacral vertebrae, Histopathological examination tumor invasion tissue, a diagnosis B-cell lymphoma made. The PVL higher, monoclonal...
Abstract In this study, we compared the effects of different bovine leukemia virus (BLV) strains and lymphocyte antigen ( BoLA ) -DRB3 alleles in cattle with enzootic leukosis (EBL) aged either <3 years or ≥3 years. The frequency infection BLV belonging to group A B-1 EBL was significantly higher than that years, regardless which BoLA-DRB3 allele present. This suggests contributes more strongly development young presence early-EBL-onset susceptibility alleles.
A homozygous calf with CACNA1S-related muscle weakness, a new autosomal recessive congenital disorder in Holstein cattle, was identified by genotyping 195 stored blood samples from calves aged less than 12 months. The patient an 8-day-old male astasia which presented to university hospital 2019. unable maintain upright position assistance stand. Congenital abnormalities the central nervous system were suspected, but necropsy revealed no specific lesion, clear diagnosis. weakness should be...
Abstract A 2-month-old Japanese Black calf exhibited mandibular and superficial cervical lymph node swelling. Fine needle aspiration cytology of the revealed large lymphoblast-like cells with mitoses. Hematological examination remarkable lymphocytosis atypical lymphocytes. Increased activities serum total lactate dehydrogenase thymidine kinase were detected. At necropsy, generalized swelling nodes was observed. Histopathological analysis diffuse proliferation medium-sized round centroblastic...
The present study analyzed B-cell clonality and bovine leukemia virus (BLV) provirus integration sites in cattle with enzootic leukosis (EBL) having BLV proviral copy numbers less or greater than the number of nucleated cells. EBL cells showed monoclonal biclonal proliferation B-cells one site. On other hand, two sites. These results suggest that superinfection can occur cattle.
A 4-year 9-month-old Holstein-Friesian dairy cow presented with anorexia. On physical examination, swelling of superficial lymph nodes, pelvic masses, and prolonged urination posture after urinating a small amount were noted. Hematological examination revealed no lymphocytosis. The bovine leukemia virus proviral load was relatively high. At necropsy, enlarged large mass in the cavity, lesions several organs observed. Histopathological proliferation neoplastic lymphocytes, which...
A 35-month-old Japanese Black cow was presented to a local veterinarian with main complaint of premature labor. There no swelling lymph nodes on the body surface and palpable mass rectal examination. Although atypical lymphocytes were observed in peripheral blood, lymphocytosis recorded. On Day 12, developed hindlimb paresis euthanized. Necropsy revealed some nodes, white nodules uterus right atrial appendage. Histopathological examination B-cell lymphoma. In clonality analysis blood tumor...
Enzootic bovine leukosis (EBL) is typically observed in cattle over 3 years old. However, some cases of EBL onset young beef have been reported Japan. The mechanism for early unclear. In Japan, are given large amounts concentrated feed with low vitamin A. Bone morphogenetic proteins (BMPs) regulators cell proliferation, differentiation, and apoptosis, thought to represent one the key players tumor malignancy. purpose this study was evaluate differences BMP-6 methylation status between under...
A 10-day-old, Japanese Black, female calf had shown astasia since just after birth. Focal symmetrical periventricular malacic lesions of the cerebrum and suppurative arthritis left hip joint were observed in macroscopic examination. Histologically, cerebral confirmed as leukomalacia (PVL). The location histological features similar to PVL humans, caused by neonatal ischemia/hypovolemia. This is first report a calf.