A5082486424- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Microbial infections and disease research
- Cytokine Signaling Pathways and Interactions
- CAR-T cell therapy research
- Herpesvirus Infections and Treatments
- Toxoplasma gondii Research Studies
- Animal Virus Infections Studies
- Animal health and immunology
- Parasitic Infections and Diagnostics
- Virus-based gene therapy research
- Ruminant Nutrition and Digestive Physiology
- Genetic and phenotypic traits in livestock
- Mycobacterium research and diagnosis
- Selenium in Biological Systems
- Veterinary medicine and infectious diseases
- Rabbits: Nutrition, Reproduction, Health
- Congenital Anomalies and Fetal Surgery
- Immune Response and Inflammation
- Infant Nutrition and Health
- Infectious Disease Case Reports and Treatments
Hokkaido Research Organization
2016-2025
National Agricultural Research Organisation
2022
Hokkaido Agricultural Research Center
2004-2011
Animal Husbandry and Veterinary Department
1997
Abstract Background Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis, which the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on dairy industry. Recently, we developed new quantitative real-time polymerase chain reaction (PCR) method using Coordination Common Motifs (CoCoMo) primers to measure proviral load known and novel variants in BLV-infected animals. Indeed, assay was highly effective detecting from...
Bovine leukemia virus (BLV), a retrovirus that is widespread worldwide, causes enzootic bovine leukosis (EBL), B-cell leukemia/lymphoma with poor prognosis ultimately results in death. In Japan, the number of cattle infected this increasing, and it estimated more than 35% are currently infected. Since no vaccines or treatments against BLV infection available, important to establish method early diagnosis for EBL reduce economic losses caused by disposal where large expensive beef raised. We...
Bovine leukemia virus (BLV) infection is a chronic viral of cattle and endemic in many countries, including Japan. Our previous study demonstrated that PGE2, product cyclooxygenase (COX) 2, suppresses Th1 responses contributes to the progression Johne disease, bacterial cattle. However, little information available on association PGE2 with infection. Thus, we analyzed changes plasma concentration during BLV its effects proviral load, gene transcription, responses, disease progression. Both...
Bovine leukemia virus (BLV), which causes enzootic bovine leukosis and immunosuppression, is widely prevalent on Japanese dairy farms. However, in the absence of a national eradication scheme with compensation programs, it important to estimate BLV-associated economic losses raise farmers' awareness. Mastitis (includes both clinical subclinical) common disease industry most reason for culling. We hypothesized that immunosuppression due BLV predisposes subclinical mastitis. A retrospective...
Abstract Introduction Bovine mycoplasma, chiefly Mycoplasma bovis , is a pathogen that causes pneumonia, mastitis, arthritis, and otitis media in cattle. This exerts immunosuppressive effects, such as the inhibition of interferon production. However, mechanisms involved bovine mycoplasmosis have not been fully elucidated. In this study, we investigated role programmed death‐1 (PD‐1)/programmed death‐ligand 1 (PD‐L1) pathway immunosuppression mycoplasmosis. Methods initial experiments, used...
CD4(+)CD25(high)Foxp3(+) T cells suppress excess immune responses that lead to autoimmune and/or inflammatory diseases, and maintain host homeostasis. However, reportedly contribute disease progression by over suppressing in some chronic infections. In this study, kinetic functional analyses of were performed cattle with bovine leukemia virus (BLV) infections, which have reported immunosuppressive characteristics. initial experiments, production the Th1 cytokines IFN-γ TNF-α was reduced...
Programmed death-1 (PD-1), an immunoinhibitory receptor on T cells, is known to be involved in immune evasion through its binding PD-ligand 1 (PD-L1) many chronic diseases. We previously found that PD-L1 expression was upregulated cattle infected with bovine leukemia virus (BLV) and antibody blocked the PD-1/PD-L1 interaction reactivated T-cell function vitro. Therefore, this study assessed antivirus activities vivo. First, we inoculated anti-bovine rat monoclonal 4G12 into a BLV-infected...
Bovine leukemia virus (BLV) is horizontally transmitted among cattle through infected blood. This 3-year field study (2013-2016) aimed to confirm the potential of blood-sucking stable fly as a risk factor BLV transmission and determine efficacy vector control on preventing BLV. The BLV-positive conversion rate during summer was higher than that winter in model dairy farm, where many flies were observed summer. After nets fixed onto barn prevent invasion, significantly decreased compared with...
Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis and closely related to human T cell (HTLV). The cytotoxic lymphocyte (CTL) plays a key role in suppressing the progression of disease caused by BLV. B epitopes BLV have been studied, but CD8(+) CTL remain poorly understood. We used library 115 synthetic peptides covering entirety Env proteins (gp51 gp30), Gag (p15, p24, p12), Tax protein identify 11 novel (gp51N5, gp51N11, gp51N12, gp30N5, gp30N6, gp30N8, gp30N16, tax16,...
Bovine leukemia virus (BLV) is a retrovirus that infects B cells in cattle and causes bovine leukosis after long latent period. Progressive exhaustion of T cell functions considered to facilitate disease progression BLV infection. Programmed death-1 (PD-1) lymphocyte activation gene-3 (LAG-3) are immunoinhibitory receptors contribute T-cell caused by infection cattle. However, it unclear whether the cooperation PD-1 LAG-3 accelerates In this study, multi-color flow cytometric analyses PD-1-...
Bovine mycoplasmosis caused by Mycoplasma bovis results in pneumonia and mastitis cattle. We previously demonstrated that the programmed death 1 (PD-1)/PD-ligand (PD-L1) pathway is involved immune dysfunction during M. infection prostaglandin E2 (PGE2) suppressed responses upregulated PD-L1 expression Johne's disease, a bacterial In this study, we investigated role of PGE2 relationship between PD-1/PD-L1 infection. vitro stimulation with expressions presumably via Toll-like receptor 2 bovine...
Bovine leukemia virus (BLV) infection is endemic in Japanese dairy farms. To promote the participation of farmers BLV control Japan, it important to provide estimates economic losses caused by this infection. We hypothesized that decreased immune function due would increase visceral abnormalities, turn reducing carcass weight. employed mediation analysis estimate annual loss weight reduction Culled Holstein cows from 12 commercial farms Nemuro and Kushiro regions Hokkaido, were traced....
Enzootic bovine leukemia is caused by the virus (BLV).BLV transmitted vertically or horizontally through transfer of infected cells via direct contact, milk, insect bites and contaminated iatrogenic procedures.However, we lacked evidence intrauterine infection.The purpose this study was to confirm BLV infection in two pregnant dams with high viral load cesarean delivery.BLV detected cord placental blood, newborns showed 100% nucleotide identity BLV-env sequence from dams.Notably, a newborn...
The effects of a maternal vaccine against newborn calf diarrhea associated with group A bovine rotavirus (BRV), coronavirus (BCV), parvovirus and K99 Escherichia coli (E. coli) were examined on beef cow-calf herd. After vaccination, serum or colostrum antibody titers to BRV, BCV E. in the vaccinated cows significantly higher than those unvaccinated control cows. Serum calves from also for 3-4 weeks after birth. These results suggested that immunization enhanced passive immunity levels coli.
Blockade of immunoinhibitory molecules, such as programmed death-1 (PD-1)/PD-ligand 1 (PD-L1), is a promising strategy for reinvigorating exhausted T cells and preventing disease progression in variety chronic infections. Application this therapeutic to cattle requires bovinized chimeric antibody targeting molecules. In study, anti-bovine PD-1 rat-bovine monoclonal 5D2 (Boch5D2) was constructed with mammalian expression systems, its biochemical function antiviral effect were characterized...
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, most common neoplastic disease cattle. BLV closely related to human T cell virus. B epitopes are important for use antibodies as therapeutic agents, epitope-driven vaccine design, and immunological assays. A epitope has not yet been found due individual differences in susceptibility.We used a peptide microarray with 156 synthetic 15-mer peptides covering envelope glycoprotein gp51 Gag proteins p15, p24, p12 map...