A5013595391- Infectious Disease Case Reports and Treatments
- Infections and bacterial resistance
- Biliary and Gastrointestinal Fistulas
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Salmonella and Campylobacter epidemiology
- Streptococcal Infections and Treatments
- Urologic and reproductive health conditions
- Antimicrobial Resistance in Staphylococcus
- Viral Infections and Vectors
- Toxin Mechanisms and Immunotoxins
- Insect symbiosis and bacterial influences
- Enterobacteriaceae and Cronobacter Research
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Pediatric Urology and Nephrology Studies
- Urological Disorders and Treatments
- Microbial Metabolism and Applications
- Insect and Pesticide Research
- Pneumonia and Respiratory Infections
- Insect and Arachnid Ecology and Behavior
- Legionella and Acanthamoeba research
- Abdominal Trauma and Injuries
- Neonatal and Maternal Infections
Kitasato University
2015-2025
University of Michigan
2006-2008
Japan Racing Association
2005
Mitsui (Japan)
2005
University of Kentucky
2005
Hokkaido University
1996-1999
University of Minnesota
1999
Mitsubishi Group (Japan)
1995
ABSTRACT The virulence plasmids of the equine virulent strains Rhodococcus equi ATCC 33701 and 103 were sequenced, their genetic structure was analyzed. p33701 80,610 bp in length, p103 1 shorter; sequences virtually identical. contained 64 open reading frames (ORFs), 22 which homologous with genes known function 3 putative unknown other species. Putative functions assigned to five ORFs based on protein family characteristics. most striking feature presence a 27,536-bp pathogenicity island...
Campylobacter jejuni infection is a leading cause of bacterial gastroenteritis in the United States and acquired primarily through ingestion contaminated poultry products. Here, we describe C. orthologue ZnuA other gram-negative bacteria. (Cj0143c) periplasmic component putative zinc ABC transport system encoded on zinc-dependent operon with Cj0142c Cj0141c, which encode two likely components jejuni. Transcription these genes dependent. A mutant lacking Cj0143c growth deficient zinc-limiting...
Campylobacter is a normal inhabitant of the chicken gut. Pathogenic infection with this organism in humans accompanied by severe inflammation intestinal mucosal surface. The aim study was to evaluate ability Lactobacillus gasseri SBT2055 (LG2055) inhibit adhesion and invasion jejuni vitro suppress C. colonization chicks vivo. Pretreatment LG2055 significantly reduced human epithelial cell line, Intestine 407, 81–176. Methanol (MeOH)-fixed also However, proteinase K (ProK)-treated eliminated...
Rhodococcus equi —a facultative intracellular pathogen of macrophages—causes bronchopneumonia in foals and patients who are immunocompromised. Virulent strains R. possess a virulence‐associated plasmid, which encodes 15‐ to 17‐kDa surface protein called A (VapA). VapA expression is regulated by temperature pH. Two transcriptional regulators, VirR VirS, involved the regulation vapA . regulates through VirS. However, whether directly virS transcription unclear. In this study, we examined...
Campylobacter jejuni has an N-linked protein glycosylation pathway that is required for efficient cell invasion and chick gastrointestinal colonization by the microbe. In this study, we constructed insertion mutants of 22 putative glycoprotein genes examined ability each to invade human intestinal epithelial line INT-407. Among tested, one carrying in Cj1496c was defective into INT-407 cells; defect also observed in-frame deletion mutant (delta Cj1496c). The delta C. showed a reduced...
Campylobacter jejuni, one of the most common causes gastroenteritis worldwide, is transmitted to humans through poultry. We previously reported that Lactobacillus gasseri SBT2055 (LG2055) reduced C. jejuni infection in human epithelial cells vitro and inhibited pathogen colonization chickens vivo. This suggested LG2055 adhesion and/or co-aggregation phenotype mediated by cell-surface aggregation-promoting factors (APFs) may be important for competitive exclusion jejuni. Here, we show cell...
Rhodococcus equi emerged as a zoonotic pathogen of human immunodeficiency virus-infected patients over the last three decades. Two virulence plasmid types R. equi, pVAPA and pVAPB associated with equine porcine isolates, have been recognized, more recently, pVAPN, novel host-associated in was found bovine caprine isolates. We reinvestigated 39 previously reported isolates from without acquired syndrome (AIDS) by detecting vapA, vapB vapN using PCR profiling. After excluding one isolate that...
We investigated the prevalence of virulent Rhodococcus equi in clinical isolates from 69 sporadic cases (60 men, 8 women, and 1 patient unknown sex) Chiang Mai, Thailand, between 1993 2001. Fifty were human immunodeficiency virus (HIV) positive, 3 HIV negative, status was for 16. Fifty-two (75%) strains intermediate virulence that contained virulence-associated 20-kDa antigen, 17 (25%) avirulent. No with 15–17-kDa antigens identified. R. isolated HIV-positive patients' houses those their...
To develop a method for typing Streptococcus equi on the basis of DNA sequence genes that produce an M-like protein and to compare isolates among United States, Japan, other countries.S strains CF32, Hidaka/95/2, NCTC9682 as well 82 from countries obtained during 1975 2001.DNA sequences structural ( SeM SzPSe) proteins were determined 3 representative find variable region. Variability in this region was then isolates. Amino acid deduced analyzed phylogenetically by use neighbor-joining...
Rhodococcus equi is a saprophytic soil bacterium and intracellular pathogen that causes refractory suppurative pneumonia in foals has emerged as pathogenic cause of zoonotic disease. Several studies have reported human infections caused by R. harboring recently described third type virulence plasmid, the ruminant-associated pVAPN, which carries vapN determinant. Herein, we analyzed pathogenicity genomic features nine vapN-harboring isolated from patients with without HIV/AIDS. Four these...
ABSTRACT Nine cat isolates and nine dog of Rhodococcus equi from clinical material were investigated for the presence virulence-associated antigens (VapA VapB) virulence plasmids. Five one isolate VapA positive contained an 85-kb type I or 87-kb plasmid. The remaining 12 avirulent R. strains no
Rhodococcus equi is a well-recognized Gram-positive intracellular facultative bacterium that opportunistic in nature, which causes pyogranulomatous infections humans and multiple host animals. The pathogenicity of the microorganism has been attributed to presence plasmid-encoded virulence-associated proteins (Vap). To date, three host-associated virulence plasmid types R. have identified as follows: circular pVAPA pVAPB, related, respectively, equine porcine isolates, recently described...
ロドコッカス・エクイ(Rhodococcus equi)は子馬に細菌性肺炎・腸炎を引き起こす細胞内寄生性のグラム陽性球桿菌で,獣医学領域ではよく知られているが,ヒトでは1968年の初発以来これまでに300を越える症例報告がある。その多くはHIV感染,臓器移植,腫瘍などの基礎疾患を持つが,健常人における感染症例も認められる。R. equiの毒力はプラスミドにコードされた毒力関連抗原(Virulence-associated protein antigens:...
ABSTRACT The plasmid types and serotypes of 164 Rhodococcus equi strains obtained from submaxillary lymph nodes swine different piggeries in 28 villages towns located throughout the country were examined. tested by PCR for presence 15- to 17-kDa virulence-associated protein antigen (VapA) 20-kDa (VapB) genes. Plasmid DNAs isolated analyzed digestion with restriction endonucleases estimate size compare their polymorphism characteristics. None isolates contained vapA gene, 44 (26.8%) positive...
Campylobacter jejuni is a leading cause of bacterial gastroenteritis in humans and commensal bacterium the intestinal tracts animals, especially poultry. Chemotaxis an important determinant for chicken colonization C. jejuni. Adaptation has crucial role gradient-sensing mechanism that underlies chemotaxis. The genome sequence reveals presence genes encoding putative adaptation proteins, CheB CheR. In-frame deletions cheB, cheR cheBR were constructed chemosensory behaviour resultant mutants...