A5060605661- Microbial Natural Products and Biosynthesis
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Biochemical and Structural Characterization
- Chemical Synthesis and Analysis
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolic Engineering and Bioproduction
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Origins and Evolution of Life
- Marine Biology and Ecology Research
- Photosynthetic Processes and Mechanisms
- Peptidase Inhibition and Analysis
- Phytochemical compounds biological activities
- Natural product bioactivities and synthesis
- Protist diversity and phylogeny
- Bacterial Infections and Vaccines
- Marine Ecology and Invasive Species
- Marine Biology and Environmental Chemistry
- Sesquiterpenes and Asteraceae Studies
- Bacterial Genetics and Biotechnology
ETH Zurich
2024-2025
Hong Kong University of Science and Technology
2019-2022
University of Hong Kong
2019-2022
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2019-2022
We conducted global genome mining of 162,672 bacterial genomes and identified 829 cyclodipeptide (CDP) biosynthesis gene clusters (BGC) containing a cytochrome P450 gene. Heterologous expression BGC from Saccharopolyspora hirsuta DSM 44795 led to the identification two novel crownlike CDPs, cyctetryptomycin A (4) B (5), which possess unprecedented complex macrocycle show neuroprotective activity. The P450s found in catalyze sequential reactions leading cyclization diketopiperazine dimers.
Streptomyces are among the most promising genera in terms of production ability to biosynthesize a variety bioactive secondary metabolites with pharmaceutical interest. Coinciding increase genomic sequencing these bacteria, mining their genomes for biosynthetic gene clusters (BGCs) has become routine component natural product discovery. Herein, we describe isolation and characterization tendae VITAKN quorum sensing inhibitory (QSI) activity that was isolated from southern coastal part India....
Marine biofouling caused huge economic losses of maritime industries. We aim to develop high-efficient, less-toxic, and cost-effective antifoulants solve the problems biofouling. In this study, we described antifouling compounds albofungin its derivatives (albofungin A, chrestoxanthone chloroalbofungin) isolated from metabolites bacterium Streptomyces chrestomyceticus BCC 24770, construction high-yield strains for production, application albofungin-based coatings. Results showed that these...
Biofilm is made up of microbes and their extracellular matrix, making microorganisms highly tolerant, resistant, resilient to a wide range antimicrobials. treatment with conventional antimicrobial agents can accelerate the evolution spread resistance due reduced efficacy increased gene transfer differentiation within biofilms. Therefore, effective biofilm-targeting compounds are currently sought after. In present study, we identified elasnin as potent compound against methicillin-resistant...
Prenylation can impart pharmacological advantages to bioactive compounds. Global genome mining for prenylated cyclodipeptides identified a gczABC BGC from Streptomyces griseocarneus 132 containing cyclodipeptide synthase and two prenyltransferase genes. Subsequent heterologous expression allowed isolation characterization of griseocazines, which displayed potent neuroprotective activity. Further biotransformation analyses revealed that prenyltransferases GczB GczC catalyzed the...
Thioamidated ribosomally synthesized and post-translationally modified peptides (RiPPs) are recently characterized natural products with wide range of potent bioactivities, such as antibiotic, antiproliferative, cytotoxic activities. These distinguished by the presence thioamide bonds in peptide backbone catalyzed YcaO-TfuA protein pair its genes adjacent to each other. Genome mining has facilitated an silico approach identify biosynthesis gene clusters (BGCs) responsible for thioamidated...
Cytochrome P450-modified bacterial terpenoids remain in a vast chemical space to be explored. In the present study, we conducted global genome mining of 223,829 genomes and identified 2892 terpenoid biosynthetic gene clusters (BGCs) with cytochrome P450 genes. Among these, selected 562 multiple enzymes, which were further clustered as 355 cluster families by sequence similarity analysis. We then chose lev, BGC from Streptomyces levis MCCC1A01616, for heterologous expression discovered four...
Abstract Microbial communities are shaped by cell-cell interactions. Even though archaea often found in associations with other microorganisms, the mechanisms structuring these poorly understood. Here we report structure and function of haloarchaeal contractile injection systems (CISs). Using a combination functional assays time lapse imaging, show that Halogeometricum borinquense exhibits antagonism towards Haloferax volcanii inducing cell lysis inhibiting proliferation. This is...
Microbial communities are shaped by cell-cell interactions. Although archaea often found in associations with other microorganisms, the mechanisms structuring these poorly understood. Here, we report on structure and function of haloarchaeal contractile injection systems (CISs). Using a combination functional assays time-lapse imaging, show that Halogeometricum borinquense exhibits antagonism toward Haloferax volcanii inducing cell lysis inhibiting proliferation. This is contact-dependent...
Abstract Biofilm is made by microbes and often offers protection making them more tolerant, resistant, resilient to wide-range antimicrobials. Biofilm-related infections account for than 80% of human bacterial are especially prevalent in chronic tissue device-related infections. Owing the great challenge treating biofilms, novel effective antibiofilm compounds urgently need be identified. We herein identified elasnin as a potent biofilm-targeting compound against methicillin-resistant...
Streptomyces, being one of the most promising genera due to its ability synthesize a variety bioactive secondary metabolites pharmaceutical interest, here studied in relation genomic and metabolomic potential. Coinciding with increase sequenced data, mining bacterial genomes for biosynthetic gene clusters (BGCs) has become routine component natural product discovery. Herein, we describe isolation characterization Streptomyces tendae VITAKN quorum sensing inhibitory activity (QSI) that was...