A5023876704- Beetle Biology and Toxicology Studies
- Effects of Radiation Exposure
- Polyamine Metabolism and Applications
- Radiation Therapy and Dosimetry
- Amino Acid Enzymes and Metabolism
- Coleoptera Taxonomy and Distribution
- Protein Structure and Dynamics
- Heat shock proteins research
- Hedgehog Signaling Pathway Studies
- Cancer Research and Treatments
- Insect and Pesticide Research
- Infrared Thermography in Medicine
- Spaceflight effects on biology
- Enzyme Structure and Function
- Radiation Effects and Dosimetry
- Bacillus and Francisella bacterial research
- Glioma Diagnosis and Treatment
- Phytochemical compounds biological activities
- EEG and Brain-Computer Interfaces
- Bee Products Chemical Analysis
- Insect Utilization and Effects
- Polyomavirus and related diseases
- thermodynamics and calorimetric analyses
- Epigenetics and DNA Methylation
- Microbial Inactivation Methods
National Agency for New Technologies, Energy and Sustainable Economic Development
2008-2024
Roma Tre University
2008-2022
Enrico Fermi Center for Study and Research
2013-2021
Istituto Superiore di Sanità
2021
Istituto Nazionale di Fisica Nucleare, Sezione di Roma I
2015
Bio-Rad (United States)
2013
Polyamine metabolism has a critical role in cell death and proliferation representing potential target for intervention breast cancer (BC). This study investigates the expression of spermine oxidase (SMO) its prognostic significance BC. Biochemical analysis Spm analogues BENSpm CPENSpm, utilized anticancer therapy, was also carried out to test their property silico vitro on recombinant SMO enzyme.BC tissue samples were analyzed transcript level activity. Student's t applied evaluate...
The most frequent interventions in cancer therapy are currently the destruction of cells by irradiation or administration drugs both able to induce radical formation and toxic metabolites enzyme-catalyzed reactions. aim this study was determine cell viability undergoing a DNA damage threshold accomplished ROS overproduction via ectopic expression murine spermine oxidase (mSMOX) bovine serum amine (BSAO) enzymes. Low dose X-irradiation delivers challenging as evaluated proficient Chinese...
Blister beetles (Coleoptera: Meloidae) are currently subdivided into three subfamilies: Eleticinae (a basal group), Nemognathinae, and Meloinae. These all characterized by the endogenous production of defensive terpene cantharidin (CA), whereas two most derived subfamilies show a hypermetamorphic larval development. Here, we provide novel draft genome assemblies five species sampled across blister beetle (Iselma pallidipennis, Stenodera caucasica, Zonitis immaculata, Lydus trimaculatus,...
Nanoparticles are being increasingly studied to enhance radiation effects. Among them, nanodiamonds (NDs) taken into great consideration due their low toxicity, inertness, chemical stability, and the possibility of surface functionalization. The objective this study is explore influence chemical/physical properties NDs on cellular radiosensitivity combined treatments with beams different energies. DAOY, a human radioresistant medulloblastoma cell line was treated NDs—differing for...
Blister beetles owe their name to ability release cantharidin, a blistering terpene, the highest concentration of which is retained in male accessory glands. The anatomy and ultrastructure three pairs reproductive glands glandular region two vasa deferentia Meloe proscarabaeus were investigated using light, electron ion beam microscopy. All mesodermal here analysed share common structural organization with an outer muscular layer inner epithelium facing broad lumen secretory products are...
Background Tissue response to irradiation is not easily recapitulated by cell culture studies. The objective of this investigation was characterize, the transcriptional and onset regenerative processes in mouse skin irradiated with different doses fast neutrons. Methodology/Principal Findings To monitor general individual animal variation, we performed gene protein expression analysis both pooled samples. A high-throughput analysis, DNA oligonucleotide microarray done three months old...
Neuroblastoma (NB) is a heterogeneous extra‑cranial childhood type of cancer, responsible for approximately 15% all paediatric cancer‑related deaths. Although several critical genetic aberrations have been related to NB, only few established molecular markers associated with prognosis [V‑myc avian myelocytomatosis viral oncogene (MYCN) locus amplification, deletions part chromosome 1p, 11q23 and gain 17q]. Regrettably, direct evidence NB‑related tumour suppressors or oncogenes has not...
Journal Article Glioblastoma stem cells: radiobiological response to ionising radiations of different qualities Get access I. Pecchia, Pecchia * 1Department Technology and Health, Istituto Superiore di Sanità, viale Regina Elena 299, Rome 00161, Italy *Corresponding author: ilaria.pecchia@iss.it Search for other works by this author on: Oxford Academic PubMed Google Scholar V. Dini, Dini Italy2Sez. Roma1-Gruppo collegato INFN, Rome, L. Ricci-Vitiani, Ricci-Vitiani 3Department Hematology,...
Low radiation doses can affect and modulate cell responses to various stress stimuli, resulting in perturbations leading resistance or sensitivity damage. To explore possible mechanisms taking place at an environmental exposure, we set-up twin biological models, one growing a low environment (LRE) laboratory the Gran Sasso National Laboratory, reference (RRE) Italian Health Institute (Istituto Superiore di Sanità, ISS). Studies were performed on pKZ1 A11 mouse hybridoma cells, which are...
Meloidae (blister beetles) are known to synthetize cantharidin (CA), a toxic and defensive terpene mainly stored in male accessory glands (MAG) emitted outward through reflex-bleeding. Recent progresses understanding CA biosynthesis production organ(s) have been made, but the way which self-protection is achieved from hazardous accumulation release of blister beetles has experimentally neglected. To provide hints on this pending question, comparative de novo assembly transcriptomic approach...
Pelvic radiation disease (PRD), a frequent side effect in patients with abdominal/pelvic cancers treated radiotherapy, remains an unmet medical need. Currently available preclinical models have limited applications for the investigation of PRD pathogenesis and possible therapeutic strategies. In order to select most effective irradiation protocol induction mice, we evaluated efficacy three different locally fractionated X-ray exposures. Using selected (10 Gy/day × 4 days), assessed through...
Hexedra+® is a nasal spray containing hydroxypropyl methylcellulose, beta-cyclodextrin, and usnic acid. It has been developed with the aim of reducing risk transmission airborne viral infections, particular reference to influenza COVID-19. As part preclinical development product, we carried out study on thirty male Wistar rats divided into three groups treated Hexedra+, an alternative formulation double concentration acid (0.015% instead 0.0075%) or saline solution. Products were...
Protons are now increasingly used to treat pediatric medulloblastoma (MB) patients. We designed and characterized a setup deliver proton beams for in vivo radiobiology experiments at TOP-IMPLART facility, prototype of proton-therapy linear accelerator developed the ENEA Frascati Research Center, with goal assessing feasibility small animal therapy research. Mice bearing Sonic-Hedgehog (Shh)-dependent MB flank were irradiated protons test whether irradiation could be restricted specific depth...
The upper part of the frequency spectrum (millimeter waves, MMW) applied by modern communications technologies (5G and beyond), makes skin dominantly exposed tissue to electromagnetic fields. In this work, a methodology for murine dosimetry evaluation is presented, intended contribute animal studies with mice MMW radiation, in particular 27.5 GHz. A stratified model proposed variations layers' thicknesses during hair cycle are measured mice. dielectric properties due age, based on changes...
Medulloblastoma (MB) is a common primary brain cancer in children. The sonic hedgehog (SHH) pathway indispensable for the normal development of cerebellum, and MB often caused by persistent SHH activation owing to mutations components. Patched1 (
Recently we have shown experimental evidence for a fragile-to-strong dynamic crossover (FSC) phenomenon in hydration water around globular protein (lysozyme) at ambient pressure. In this letter show that when applying pressure to the protein-water system, FSC temperatures of lysozyme tracks similar Widom line emanating from existence liquid-liquid critical point 1-D confined (in MCM-41-S). The mean squared displacements (MSD) hydrogen atoms and its sudden change slopes same characteristic...